Ganguly, S* Dotaz Zobrazit nápovědu
- MeSH
- acetylglukosaminidasa metabolismus MeSH
- alkalická fosfatasa metabolismus MeSH
- glukuronidasa metabolismus MeSH
- lepra enzymologie krev patofyziologie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
Biochemica Society Symposia ; nu. 35
479 s. : il.
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biochemie
- MeSH
- hepatitida B - antigeny krev MeSH
- infekční komplikace v těhotenství imunologie MeSH
- regulační T-lymfocyty krev MeSH
- těhotenství MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- srovnávací studie MeSH
Anti-trichomonad IgA antibodies were estimated by enzyme-linked immunosorbent assay (ELISA) in serum and vaginal secretions of 25 symptomatic and 25 asymptomatic Trichomonas vaginalis positive patients before and after treatment and in 25 age-matched controls. Significantly higher levels of antitrichomonad IgA antibodies were found in T. vaginalis positive patients when compared to control subjects, especially in vaginal secretions. In addition, a significant decrease in these antibodies was observed after treatment, which was more pronounced in vaginal secretions. It seems that anti-trichomonad IgA antibodies in serum and more so in vaginal secretions are directly related to and specific to the presence of T. vaginalis in the urogenital tract.
- MeSH
- dospělí MeSH
- ELISA MeSH
- imunoglobulin A analýza MeSH
- lidé MeSH
- protilátky protozoální analýza MeSH
- trichomonádová vaginitida farmakoterapie imunologie MeSH
- Trichomonas vaginalis imunologie MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The role of a 120-kb plasmid in relation to virulence and drug resistance factor in Shigella dysenteriae was studied. For characterization of plasmids, the mating system is a useful and efficient means of transferring both large and small plasmids to a new host. The conjugative transfer of a 120-kb (pCAT120) ampicillin-resistant plasmid of S. dysenteriae to E. coli K-12 was not successful. Introduction of an E. coli fertility factor plasmid F, did not help to mobilize the plasmid. Low transfer frequencies of antibiotic markers to E. coli were achieved by treatment of the donor S. dysenteriae with N-methyl-N'-nitro-N-nitrosoguanidine. The transconjugants showed resistance to ampicillin, chloramphenicol, tetracycline and cadmium. A transconjugant carrying the 120-kb plasmid of S. dysenteriae produced keratoconjunctivitis in guinea pigs. Repeated subculture of Clmr transconjugant (pCAT120) on tryptic soya agar plates became Clms and showed four distinct DNA bands ranging from 3 to 10 kb in size on agarose gel electrophoresis. Utilization of organic acids, metal resistance (Cd), dye-binding properties (Crb+, Ebr+) and drug resistance (Amp, Tet) were identified on 10, 7, 4 and 3-kb plasmid DNA fragment of pCAT120 respectively. Crb+ 4-kb DNA fragment of pCAT120 was isolated, purified and transferred to an avirulent E. coli K12 by transformation. However, transformant (pET4) showed poor growth on solid media and its growth in liquid culture was only possible after supplementation of the unknown low-molar-mass thermolabile factor(s) secreted by the recipient strain.(ABSTRACT TRUNCATED AT 250 WORDS)
