Strains identified in ovine cheese and bryndza by matrix-assisted laser desorption/ionization time-of-flight analysis belonged to ten species of non-enterococcal lactic acid bacteria and included Lactobacillus casei/Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus helveticus, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus brevis, Lactococcus lactis, Pediococcus pentosaceus and Pediococcus acidilactici. The susceptibility toward antibiotics was determined in lactobacilli, lactococci and pediococci and also in Escherichia coli for comparison. Analysis of L. fermentum and pediococci revealed the presence of non-wild-type epidemiological cut-offs in streptomycin, clindamycin or gentamicin. E. coli were resistant to ampicillin, tetracycline, enrofloxacin and florfenicol. No extended spectrum β-lactamases were detected.

• MeSH
• antibakteriální látky farmakologie   MeSH
• Bacteria účinky léků   genetika   izolace a purifikace   MeSH
• mikrobiální testy citlivosti MeSH
• ovce MeSH
• sýr mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Fungi are commonly involved in dairy product spoilage and the use of bioprotective cultures can be a complementary approach to reduce food waste and economic losses. In this study, the antifungal activity of 89 Lactobacillus and 23 Pediococcus spp. isolates against three spoilage species, e.g., Yarrowia lipolytica, Rhodotorula mucilaginosa and Penicillium brevicompactum, was first evaluated in milk agar. None of the tested pediococci showed antifungal activity while 3, 23 and 43 lactobacilli isolates showed strong antifungal activity or total inhibition against Y. lipolytica, R. mucilaginosa and P. brevicompactum, respectively. Then, the three most promising strains, Lactobacillus paracasei SYR90, Lactobacillus plantarum OVI9 and Lactobacillus rhamnosus BIOIII28 at initial concentrations of 105and 107CFU/ml were tested as bioprotective cultures against the same fungal targets in a yogurt model during a 5-week storage period at 10 °C. While limited effects were observed at 105CFU/ml inoculum level, L. paracasei SYR90 and L. rhamnosus BIOIII28 at 107CFU/ml respectively retarded the growth of R. mucilaginosa and P. brevicompactum as compared to a control without selected cultures. In contrast, growth of Y. lipolytica was only slightly affected. In conclusion, these selected strains may be good candidates for bioprotection of fermented dairy products.

• MeSH
• antifungální látky farmakologie   MeSH
• Lactobacillus fyziologie   MeSH
• mléčné výrobky mikrobiologie   MeSH
• Pediococcus fyziologie   MeSH
• Penicillium růst a vývoj   MeSH
• potravinářská mikrobiologie MeSH
• probiotika MeSH
• Rhodotorula růst a vývoj   MeSH
• Yarrowia růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH

A rifampicin-resistant variant of two strains of Lactobacillus plantarum, one strain of Pediococcus acidilactici, and one strain of Enterococcus faecium were used for the experimental production of lucerne silage. Laboratory silage without inoculants served as a control. Counts of total anaerobes, total lactic acid bacteria (LAB), lactobacilli, pediococci, and enterococci were determined on days 14, 21, 30, 49, and 60 of lucerne fermentation. LAB dominated in silage microflora, reaching a percentage between 59 and 95 % of total anaerobes. Lactobacilli were found as a predominant group of LAB during the whole study. Lactobacilli reached numbers 8.74 log CFU/g in treated silage and 8.89 log CFU/g in the control at the first observation. Their counts decreased to 4.23 and 4.92 log CFU/g in treated silage and the control, respectively, on day 63 of fermentation. Similar decreases were observed in all bacterial groups. The treated silage samples possessed lower pH (4.2 vs. 4.5 in control samples) and contained more lactic acid compared to control silage. The identity of re-isolated rifampicin-resistant bacteria with those inoculated to the lucerne was evaluated by fingerprinting techniques. The fingerprint profiles of re-isolated bacteria corresponded to the profiles of strains used for the treatment. It could be concluded that supplemented LAB dominated in laboratory silage and overgrew naturally occurring LAB.

• MeSH
• fermentace MeSH
• kyselina mléčná metabolismus   MeSH
• Lactobacillaceae růst a vývoj   izolace a purifikace   metabolismus   MeSH
• Medicago sativa metabolismus   mikrobiologie   MeSH
• mikrobiální viabilita MeSH
• siláž mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Konspekt
• Mikrobiologie
• NLK Obory
• mikrobiologie, lékařská mikrobiologie
• NLK Publikační typ
• učebnice vysokých škol

• MeSH
• bezpečnost potravin MeSH
• hygiena MeSH
• kontaminace potravin MeSH
• laboratoře MeSH
• mikrobiologické techniky MeSH
• potravinářská mikrobiologie MeSH
• průmysl zpracování potravin MeSH

• Konspekt
• Učební osnovy. Vyučovací předměty. Učebnice
• Mikrobiologie
• NLK Obory
• hygiena
• zemědělství a potravinářství
• biochemie
• mikrobiologie, lékařská mikrobiologie
• NLK Publikační typ
• učebnice vysokých škol
• praktická cvičení

Vysokoškolská učebnice, která se zaměřuje na různé druhy mikroorganismů a jejich ekologii a patogenitu.

• MeSH
• ekologie MeSH
• infekční nemoci MeSH
• mikrobiologické jevy MeSH
• mikrobiologie životního prostředí MeSH
• molekulární biologie MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Mikrobiologie
• Učební osnovy. Vyučovací předměty. Učebnice
• NLK Obory
• mikrobiologie, lékařská mikrobiologie
• NLK Publikační typ
• učebnice vysokých škol