Q112343012
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A liquid marble is a liquid droplet encapsulated in a hydrophobic powder that adheres to the liquid surface. Liquid marbles preparation is very simple – a small amount of liquid is carefully dripped on the layer of hydrophobic powder consisting of nano- or micro particles, which spread spontaneously at the interface liquid/air. This process results in a liquid marble that has some of the properties of a liquid droplet and, at the same time, behaves as a soft solid. Liquid marbles present an alternative to superhydrophobic surfaces because these particles prevent the liquid to wet and contaminate the carrier surface, be it solid or liquid. The present work focuses on the description of basic properties of liquid marbles; also, an overview is given of possible applications of liquid marbles, e.g. for the transport of small volumes of liquids or powders in microfluidics, for the detection of gases or water contamination or as (bio)microreactors.
The ability to simulate the 3D structure of a human body is essential to increase the efficiency of drug development. In vivo conditions are significantly different in comparison to in vitro conditions. A standardly used cell monolayer on tissue culture plastic (2D cell culture) is not sufficient to simulate the transfer phenomena occurring in living organisms, therefore, cell growth in a 3D space is desired. Drug absorption, distribution, metabolism, excretion and toxicity could be tested on 3D cell aggregates called spheroids, decrease the use of animal models and accelerate the drug development. In this work, the formation of spheroids from HT-29 human colorectal adenocarcinoma cells was successfully achieved by means of the so-called liquid marbles, which are liquid droplets encapsulated by a hydrophobic powder. During the cultivation in the medium inside the liquid marbles, cells spontaneously formed spherical agglomerates (spheroids) without the need of any supporting scaffold. The study focused on the influence of different parameters—namely liquid marble volume, seeding cell density and time of cultivation—on the final yield and quality of spheroids. This work has shown that using liquid marbles as microbioreactors is a suitable method for the cultivation of HT-29 cells in the form of spheroids.
- Klíčová slova
- liquid marble,
- MeSH
- bioreaktory MeSH
- buněčné sféroidy MeSH
- buňky HT-29 MeSH
- lidé MeSH
- techniky 3D buněčné kultury * metody MeSH
- uhličitan vápenatý MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Recent advances in the fabrication, characterization and application of micro- and nano-particles that possess a non-uniform internal structure are reviewed. The particle structures include core-shell particles, particles with multiple cores or a multi-layered structure, porous particles with both regular and random pore structure, as well as complex composite particles possessing several of the above features. Particles based on "hard" inorganic materials such as silica, "soft" organic materials such as polymers and their composites are considered. The fabrication approaches include bottom-up self-assembly techniques, templating methods, microfluidics, and various emulsion-based routes to structured micro- and nano-particle formation, combined with both physical (e.g. Pickering emulsions) and chemical (e.g. polymerization, precipitation) processes for the material deposition. The applications of the structured particles for the encapsulation and controlled delivery of active substances are then reviewed with emphasis on those systems where the complex particle structure can provide specific benefits such as in-situ formation of the active substance or precise control over the release profile. Likely future research directions and prospects are discussed.
- MeSH
- léčivé přípravky aplikace a dávkování chemie MeSH
- lékové transportní systémy * MeSH
- lidé MeSH
- mikrosféry MeSH
- nanočástice MeSH
- oxid křemičitý chemie MeSH
- polymery chemie MeSH
- poréznost MeSH
- příprava léků metody MeSH
- racionální návrh léčiv MeSH
- velikost částic MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
