Q74093280
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Adsorpcí biopolymerů na povrch titanu a titanem dopovaných uhlovodíkových povlaků se zvyšuje optická drsnost, tloušťka a index lomu povrchové vrstvy. Fibrinogen se lépe adsorbuje na povrchu titanu, který je upraven leštěním a leptáním, nežli na povrchu pouze leštěném. Nejlépe se adsorbuje na povrch uhlovodíkových povlaků dopovaným titanem, které byly deponované reaktivním magnetronovým naprašováním v optimálním poměru Ti0,38 - C0,62 a Ti0,09 - C0,91. Takto upravené povrchy dentálních implantátů by měly urychlit jejich oseointegraci a vhojení. Na povrch titanu se lépe adsorbují jednořetězové pyrimidinové oligodeoxynukleotidy (TTC)12, nežli purinové (AAG)12. Dvojšroubovicový duplex (TTC)12. (AAG)12 neovlivňuje optické vlastnosti povrchu titanu a patrně se na povrch neadsorbuje.
Adsorption of biopolymers at the titanium and titanium carbide surface increases the optical roughness as well as the thickness and refractive index of the surface layer. Fibrinogen is better adsorbed at the titanium surface which is treated by polishing and etching than at the surface treated only by polishing. The best adsorption of fibrinogen was observed at the titanium carbide surface prepared by plasma-enhanced chemical vapour deposition, the optimal ratio was Ti0,38 - C0,62 a Ti0,09 - C0,91. The surface of dental implants treated by this way should speed up their osseointegration and healing. The single stranded pyrimidine oligodeoxynucleotides (TTC)12 are better adsorbed at the titanium surface than the purine oligodeoxynucleotides (AAG)12. The double-helical duplex (TTC)12. (AAG)12 has no effect on the optical properties of the titanium surface and probably is not adsorbed there.
- MeSH
- adsorpce MeSH
- biokompatibilní materiály normy MeSH
- biosenzitivní techniky využití MeSH
- fibrinogen MeSH
- financování organizované MeSH
- mikroskopie atomárních sil využití MeSH
- oligonukleotidy MeSH
- osteoblasty ultrastruktura MeSH
- osteointegrace fyziologie MeSH
- techniky in vitro MeSH
- titan terapeutické užití MeSH
- zubní implantáty MeSH
Stripping voltammetric determination of purine bases in the presence of copper ions at mercury, amalgam, or carbon-based electrodes has recently been utilized in analysis of DNA or synthetic oligodeoxynucleotides (ODNs). Here we report on copper-enhanced label-free anodic stripping detection of guanine and adenine bases in acid-hydrolyzed DNA at anodically oxidized boron-doped diamond electrode (AO-BDDE). The AO-BDDE was successfully applied in a three-electrode microcell in which an approximately 50 microL drop of the analyte solution can be efficiently stirred during the accumulation step by streaming of an inert gas. Accelerated mass transport due to the solution motion in the presence of copper resulted in enhancement of the guanine oxidation signal by about 2 orders of magnitude (compared to accumulation of the analyte from still solution not containing copper), allowing an easy detection of approximately 25 fmol of the ODNs. The proposed technique is shown to be suitable for a determination of purine (particularly guanine) content in DNA samples. Applications of the technique in magnetic bead-based DNA assays (such as hybridization with DNA sequences exhibiting asymmetrical distribution of purine/pyrimidine nucleotides between the complementary strands or monitoring of amplification of specific DNA fragments in a duplex polymerase chain reaction) are demonstrated.
- MeSH
- bor chemie MeSH
- diamant chemie MeSH
- DNA-dependentní DNA-polymerasy metabolismus MeSH
- DNA MeSH
- elektrochemie MeSH
- elektrody MeSH
- financování organizované MeSH
- hydrolýza MeSH
- kationty chemie MeSH
- kyseliny chemie MeSH
- měď chemie MeSH
- oligonukleotidy chemie MeSH
- oxidace-redukce MeSH
- puriny chemie MeSH
- sekvence nukleotidů MeSH
