• Publikační typ
• abstrakt z konference MeSH

• Publikační typ
• abstrakt z konference MeSH

Východiska: Prevalence karcinomů hlavy a krku v posledních dekádách stoupá, což představuje vážný sociální i medicínský problém. Retrospektivní hodnocení kvality života po léčbě brachyterapií s vysokým dávkovým příkonem (high-dose rate – HDR) u pacientů s časnými nádory dutiny ústní. Materiál a metody: Kvalita života byla hodnocena u 14 pacientů léčených HDR brachyterapií pro časné nádory dutiny ústní (jazyk, spodina dutiny ústní) léčených na klinice Onkologie a radioterapie LF UK a FN Hradec Králové. Pro hodnocení byly použity dotazníky EORTC QLQ-C30 a EORTC QLQ-H&N 35. Hodnocení bylo provedeno 12 měsíců po léčbě. Všichni pacienti podepsali před zařazením do studie informovaný souhlas. Dotazníky pacienti vyplňovali samostatně, bez asistence zdravotnického personálu. Výsledky: Celkový zdravotní stav podle EORTC QLQ-C30 byl hodnocen 70 body. Nejzávažnějšími symptomy byly únava, nespavost, ztráta chuti k jídlu, dušnost a finanční problémy. EORTC QLQ-H&N 35 identifikoval jako hlavní problémy přibírání na váze, kašel, bolest, lepkavé sliny a problémy s jídlem ve společnosti. Naopak ztráta na váze, porucha řeči, otvírání úst, polykání a senzorické obtíže hrály nevýznamnou roli. Závěr: HDR brachyterapie představuje efektivní metodu léčby časných nádorů dutiny ústní s uspokojivou kvalitou života. Bylo by prospěšné iniciovat prospektivní multicentrické multidisciplinární studie zaměřené na kvalitu života pacientů s nádory hlavy a krku, kteří jsou léčeni různými způsoby léčby, a pak provést jejich porovnání.

Background: The prevalence of head and neck tumors has continually increased over the past decades, which is a serious medical and social problem. This study retrospectively assessed the quality of life of patients with early-stage oral cavity tumors after high-dose rate (HDR) brachytherapy. Material and Methods: Quality of life was evaluated in 14 patients with early-onset oral cavity tumors who underwent HDR brachytherapy at the Department of Oncology and Radiotherapy, University Hospital Hradec Kralove. The standardized EORTC QLQ-C30 and EORTC QLQ-H&N 35 questionnaires were used. Patients completed these questionnaires at 12 months after treatment. Results: The general health status was scored 70 points using the EORTC QLQ-C30 questionnaire. Fatigue, insomnia, loss of appetite, dyspnea, and financial problems were the most severe problems reported. The main problems identified by the EORTC QLQ-H&N 35 questionnaire were weight gain, coughing, pain, sticky saliva, and difficulties eating in public. On the contrary, patients did not significantly suffer weight loss, speech impairments, difficulties in mouth opening or swallowing, or sensory problems. Conclusion: HDR brachytherapy is an effective treatment for early oral cancer that leaves patients with an acceptable quality of life. A prospective, multicentric study focused on the quality of life of patients with head and neck tumors is required.

• MeSH
• brachyterapie * metody   MeSH
• dospělí MeSH
• kvalita života * MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory úst * radioterapie   MeSH
• průzkumy a dotazníky MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH

Despite the huge research into stem cells and their regenerative properties for bone healing, there are still unanswered questions including the recipient's respond to the presence of the stem cells, the fate of stem cells inside the bone defect and the possible advantage in utilizing pre-differentiated cells. To address these problems, we used human multipotent mesenchymal stromal/stem cells (MSCs), GMP Grade, in a rat model of bone formation. In a "bioreactor concept" approach seven Wistar rats were implanted with 0.2 g of synthetic bone scaffold seeded with 2 × 106 MSCs, seven Wistar rats were implanted with 0.2 g of synthetic bone scaffold seeded with 1 × 106 predifferentiated osteoblasts and 1 × 106 pre-differentiated endothelial cells and 14 Wistar rats were implanted with 0.2 g of synthetic bone scaffold without seeded cells into an intramuscular pocket on the left side of their back. The right side of each rat was used as a control, and 0.2 g of synthetic bone scaffold was implanted into the intramuscular pocket alone. To see the early stage healing the samples were harvested 14 days after the implantation, MSCs were detected by positive DAPI and MTCO2 staining in 43% of all the samples implanted with MSCs, and no inflammation signs were present in any implanted animal. New vessels could be found in both groups implanted with MSCs, but not in the control group of animals. However, hematoxylin-eosin staining could not detect newly created bone within the implant in any of the groups. These results were in line with COLL1 staining, where we could detect positive staining only in three cases, all of which were implanted with un-differentiated MSCs. According to our findings, there were no benefits of using the pre-differentiated of MSC.

• MeSH
• fyziologická neovaskularizace MeSH
• mezenchymální kmenové buňky fyziologie   MeSH
• osteoblasty fyziologie   MeSH
• osteogeneze fyziologie   MeSH
• potkani Wistar MeSH
• regenerace kostí fyziologie   MeSH
• transplantace mezenchymálních kmenových buněk metody   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

INTRODUCTION: Cellulitis remains a very serious disease even today. Mortality, which varied between 10-40%, has been reduced owing to the standard securing of airway patency and use of an appropriate surgical treatment approach. MATERIALS AND METHODS: A total of 195 patients were hospitalised for cellulitis at the University Hospital in Hradec Králové during 2007-2011. The following parameters were evaluated: age, gender, dependence of incidence of the disease on the season of the year, frequency of attacks of the particular areas and their clinical characteristics, aetiology of the inflammation, types of patient complaints, prevalence of current systemic diseases, results of microbiological and selected laboratory analyses, socio-economic status of the patients, and duration of patient stay at the hospital. Statistical analysis was performed by using Pearson's correlation coefficient, the statistical significance level was p < 0.05. RESULTS: The mean age of the patients was 39.8 years. The group of 195 patients included 108 (55%) males and 87 (45%) females. The mean time between the first symptoms of the disease and admission to the Department was 5 days. From among the 195 patients, 116 (59.5%) were working persons, 79 (40.5%) were non-working (children, students, unemployed persons, women on maternity leave, retired people). The odontogenic origin of the disease was verified in 173 (88.7%) patients. In total, 65 (33.3%) patients had no coinciding complicating systemic disease, 22 (11.3%) patients had diabetes mellitus. The most frequent symptom of cellulitis was painful swelling, found in 194 (99.5%) patients, followed by jaw contracture, found in 153 (78.5%) patients. CONCLUSION: The results are largely very similar to those of previous studies performed in other countries, except that we found no correlation between the prevalence of cellulitis and the socio-economic status, nor have we confirmed Klebsiella pneumoniae sp. as the cause of cellulitis in patients with diabetes mellitus.

• MeSH
• celulitis diagnóza   epidemiologie   mikrobiologie   MeSH
• dítě MeSH
• dospělí MeSH
• krk * MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• nemoci zubů komplikace   mikrobiologie   MeSH
• předškolní dítě MeSH
• retrospektivní studie MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• socioekonomické faktory MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

The aim of this study is to evaluate the biologic importance and prognostic significance of selected clinicopathological parameters in patients with oral (OSCC) and oropharyngeal (OPSCC) squamous cell carcinoma, with emphasis on smoking, protein p16(INK4a) (p16) expression, and human papillomavirus (HPV) status.The study sample consisted of 48 patients with OSCC and 44 patients with OPSCC. Half of the patients were nonsmokers and the other half were gender-, age- and tumor localization-matched smokers. p16 expression was detected in 17/48 (35 %) OSCCs and in 36/44 (82 %) OPSCCs and HPV DNA was present in 7/48 (15 %) OSCCs and in 35/44 (80 %) OPSCCs. The sensitivity and specificity of p16 expression for HPV DNA presence were 0.74 and 0.88, respectively. The OPSCCs were more frequently basaloid (p < 0.001) while the OSCCs were more frequently conventional (p < 0.000001). The OSCCs were more likely to recur locally and to be the cause of death (p = 0.009 in both parameters).The HPV-positive tumors were more frequently localized in oropharynx, were basaloid SCCs and were p16- and HPV-positive (p < 0.000001 in all 4 parameters). The HPV-negative tumors were more frequently localized in oral cavity (p < 0.000001), more frequently asociated with local, regional and locoregional recurence (p = 0.011, p = 0.019 and p = 0.030, respectively) and with tumor-related death (p = 0.003). There was no significant difference with regard to smoking history (p > 0.05). The survival of patients with HPV-positive tumors was significantly longer (median 112 months; 95% CI 54 - 112 months) than that of patients with HPV-negative tumors (median 17 months; 95% CI 12 - 39 months) (p < 0.001). The HPV status of OSCC/OPSCC is an important biological and prognostic parameter and should be examined in all cases, using PCR or immunohistochemical detection of surrogate marker p16. Smoking itself does not seem to be an important prognostic factor.

• MeSH
• DNA virů MeSH
• dospělí MeSH
• imunoenzymatické techniky MeSH
• infekce papilomavirem metabolismus   mortalita   virologie   MeSH
• inhibitor p16 cyklin-dependentní kinasy metabolismus   MeSH
• kouření škodlivé účinky   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lokální recidiva nádoru metabolismus   mortalita   virologie   MeSH
• míra přežití MeSH
• nádory orofaryngu metabolismus   mortalita   virologie   MeSH
• nádory úst metabolismus   mortalita   virologie   MeSH
• Papillomaviridae genetika   izolace a purifikace   MeSH
• polymerázová řetězová reakce MeSH
• prognóza MeSH
• rizikové faktory MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• spinocelulární karcinom metabolismus   mortalita   virologie   MeSH
• staging nádorů MeSH
• studie případů a kontrol MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

Kongenitální epulida z granulárních buněk je vzácným nezhoubným nádorem novorozeneckého věku vycházejícím z tkání dutiny ústní. Při určité velikosti útvaru je diagnostika této afekce možná již prenatálně pomocí moderních zobrazovacích metod. Je-li tumor menší, je jeho přítomnost zjištěna až při porodu či krátce po něm. Terapie spočívá v chirurgickém odstranění afekce, doba vhodná k jejímu odstranění závisí na velikosti a lokalizaci epulidy. Afekce není sdružena s žádnými vrozenými vývojovými vadami, po odstranění nerecidivuje a nezanechává trvalé následky.

Congenital epulis coming of granular cells is rare non-malignant tumor of neonatal age proceeded from oral cavity tissues. In certain size of the tumor diagnosis is possible prenatally using the recent visualizing methods. In case of small size of the lesion tumor is diagnosed perinatally or postnatally. The treatment of choice is surgical intervention depends on the size a localization of the epulis. The lesion is not associated with other congenital developmental defect. There is no evidence of reccurence and subsequent complications after the surgical removal.

• Klíčová slova
• kongenitální epulida, granulární buňky, prenatální diagnostika.,
• MeSH
• císařský řez MeSH
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• matky MeSH
• nádory dásní * diagnóza   etiologie   chirurgie   terapie   MeSH
• novorozenec růst a vývoj   MeSH
• péče o matku MeSH
• prenatální diagnóza MeSH
• vrozené vady epidemiologie   MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• novorozenec růst a vývoj   MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

• Publikační typ
• abstrakt z konference MeSH

The aim of the study was to investigate the role of high-risk human papillomavirus (HR-HPV) infection in the etiopathogenesis of oral (OSCC) and oropharyngeal (OPSCC) squamous cell carcinoma in non-smoking and non-drinking patients (NSNDP). Twenty-four OSCCs and 22 OPSCCs were analyzed by immunohistochemistry for p16(INK4a) protein (p16) expression and by chromogene in situ hybridization (CISH) and polymerase chain reaction (PCR) for HR-HPV DNA presence. The series included 23 males and 23 females aged 35-93 years. p16 expression was seen in 7 out of 24 (29%) OSCCs and in 22 out of 22 (100%) OPSCCs. Using CISH, HR-HPV DNA was observed in 6 out of 24 (25%) OSCCs and in 21 out of 22 (95%) OPSCCs. HPV DNA was found in 3 out of 24 (13%) OSCCs and in 18 out of 22 (82%) OPSCCs using PCR. HPV 16 and 33 were detected in 16 and in two cases, respectively. Compared with OSCCs, OPSCCs more frequently showed basaloid morphology (p < 0.0001), lymph node involvement (p = 0.0063), diffuse p16 expression (p < 0.0001), HR-HPV DNA presence using both CISH and PCR (p < 0.0001; p < 0.0001), and better outcome. The sensitivity and specificity of p16 expression for HR-HPV DNA presence detected by CISH were 0.89 and 0.95, respectively, and 0.95 and 0.85 for PCR detected HPV DNA. The sensitivity and specificity of CISH for PCR detected presence of HPV DNA were 1.00 and 0.73, respectively. Our study is the first larger study analyzing OSCC and OPSCC in NSNDP. Our results indicate that unlike OSCC, a vast majority of OPSCCs may be associated with HR-HPV infection.

• MeSH
• DNA virů analýza   MeSH
• dospělí MeSH
• hybridizace in situ MeSH
• imunohistochemie MeSH
• infekce papilomavirem komplikace   MeSH
• inhibitor p16 cyklin-dependentní kinasy analýza   biosyntéza   MeSH
• kouření MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory orofaryngu etiologie   patologie   virologie   MeSH
• nádory úst etiologie   patologie   virologie   MeSH
• pití alkoholu MeSH
• polymerázová řetězová reakce MeSH
• rizikové faktory MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• senzitivita a specificita MeSH
• spinocelulární karcinom etiologie   patologie   virologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Low-grade cribriform cystadenocarcinoma of salivary glands is a recently described rare tumor with favorable prognosis. This study reports the case of 50-year-old woman with swelling lasting for 9 months in the right parotideomasseteric area. Grossly, the tumor was well circumscribed and dominated by cystic space. Microscopically, the neoplasm consisted of well-demarcated islets, some of them cystically dilated. The architecture of islets varied from solid to cribriform and micropapillary without comedo-type necroses. The tumor cells featured no significant cytologic atypia. Immunohistochemically, luminal cells showed expression of cytokeratins (CK), CK7, CK18, and S100 protein. In addition, immunostains for CK5/6, CK14, p63 protein, and smooth muscle actin displayed a continuous rim of myoepithelial cells around all tumor nests. In contrast, detection of CK20, hormonal receptors (androgen, estrogen, and progesterone), epidermal growth factor receptor and Her-2/neu oncoprotein was negative. The patient is free of disease for 2 years. The relationship between low-grade cribriform cystadenocarcinoma and salivary duct carcinoma is discussed.

• MeSH
• cystadenokarcinom metabolismus   patologie   chirurgie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádorové biomarkery metabolismus   MeSH
• nádory příušní žlázy metabolismus   patologie   chirurgie   MeSH
• nádory slinných žláz patologie   MeSH
• přežití bez známek nemoci MeSH
• prognóza MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH