Q95374704
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- MeSH
- insekticidy aplikace a dávkování farmakologie MeSH
- kontrola škůdců metody MeSH
- nosatcovití * patogenita účinky léků MeSH
- ochrana úrody metody MeSH
- oleje prchavé * aplikace a dávkování farmakologie MeSH
- pšenice MeSH
- rozmarýn MeSH
- statistika jako téma MeSH
- Thymus (rostlina) MeSH
- výzkumné techniky MeSH
- výzkumný projekt MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- deratizace MeSH
- dýchací soustava * MeSH
- hlodavci * fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Klíčová slova
- pisivky, kontejnery,
- MeSH
- dezinsekce * metody MeSH
- doprava přístrojové vybavení MeSH
- dusík MeSH
- hmyz MeSH
- kontrola škůdců MeSH
- Klíčová slova
- rušník obilní,
- MeSH
- dezinsekce * metody MeSH
- dusík MeSH
- hmyz MeSH
- kontrola škůdců MeSH
- skladování potravin MeSH
- výzkum MeSH
Anticoagulants are the most frequently used rodenticides at the global scale. Because of their persistency, bioaccumulation and potential for secondary intoxication, they have faced increasing legislative regulations. Recently, the European Union Regulation (EU) 2016/1179 resulted in the production and application of rodenticides with nearly half dose (<30 ppm) of anticoagulants. However, published data on the biological efficacy of rodenticides with decreased doses are scarce in the EU. Therefore, this work compared the efficacy of the original high-dose (50 ppm) and new low-dose (25 ppm) brodifacoum-based baits in the offspring of wild-caught house mice (Mus musculus L.). In the no-choice laboratory feeding tests, 100% animals died in all treated groups and 0% died in the control groups. The achieved time to death did not differ between the original and low-dose baits across both types of feeding trials/regimes. The low-dose baits (25 ppm) were consequently tested under field conditions in two populations showing 95.7% and 99.8% efficacy. The obtained results highlighted the good efficacy of the new baits based on low-dose brodifacoum in non-resistant mouse populations. However, further validation is required regarding the remaining anticoagulant compounds and resistant rodent populations.
- MeSH
- 4-hydroxykumariny chemie toxicita MeSH
- antikoagulancia chemie toxicita MeSH
- Evropská unie MeSH
- krmivo pro zvířata MeSH
- mortalita MeSH
- myši MeSH
- rodenticidy chemie toxicita MeSH
- výpočet dávky léku MeSH
- zákonodárství lékové MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- deratizace * metody MeSH
- kontrola škůdců metody MeSH
- rodenticidy aplikace a dávkování MeSH
- Publikační typ
- práce podpořená grantem MeSH
Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damage stored products and cause serious economic loss. However, because of the body size (~1 mm) of eggs, nymphs, and adults, morphological identification of most stored-product psocids is difficult and hampers effective identification. In this study, 10 economically important stored-product Liposcelis spp. psocids (Liposcelis brunnea, L. entomophila, L. decolor, L. pearmani, L. rufa, L.mendax, L. bostrychophila, L. corrodens, L. paeta, and L. tricolor) were collected from 25 geographic locations in 3 countries (China, Czech Republic, and the United States). Ten species-specific probes for identifying these 10 psocid species were designed based on ITS2 sequences. The microarray method and reaction system were optimized. Specificity of each of the ten probes was tested, and all probes were found suitable for use in identification of the respective10 Liposcelis spp. psocids at 66 °C. This method was also used to identify an unknown psocid species collected in Taian, China. This work has contributed to the development of a molecular identification method for stored-product psocids, and can provide technical support not only to facilitate identification of intercepted samples in relation to plant quarantine, but also for use in insect pest monitoring.
- MeSH
- DNA sondy chemie metabolismus MeSH
- druhová specificita MeSH
- hmyz genetika MeSH
- ribozomální DNA chemie metabolismus MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH