• MeSH
• acetylcholinesterasa krev   účinky léků   MeSH
• chemické bojové látky MeSH
• krysa rodu rattus MeSH
• látky ovlivňující centrální nervový systém aplikace a dávkování   farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

• MeSH
• hypertenze MeSH
• srdeční selhání MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• kardiologie
• NLK Publikační typ
• kolektivní monografie

BACKGROUND: Extreme efforts are made for the structural diversification of oximes used as AChE reactivators. Co-administration of different oximes should also be considered as a solution in therapy. Consequently, development of selective assays of oximes in biological matrices is of major importance. RESULTS: Three chromatographic separation mechanisms were evaluated: hydrophilic-interaction LC; mixed reversed-phase/cation exchange (DUET); and reversed-phase ion pairing based on per-fluorinated agents. MS was used to identify and quantify oximes. Alternative preparation of whole blood and plasma samples were used based on protein precipitation through addition of acetonitrile or ionic liquids. Quality characteristics of the proposed analytical approaches are discussed. CONCLUSION: The reversed-phase ion pairing based on per-fluorinated agents chromatographic separation mechanism and positive ESI-MS/MS detection produced the best results for the assay of bis-quaternary pyridinium oximes. LLOQ in the tenths of nanogram per milliliter range are achievable.

• MeSH
• acetylcholinesterasa chemie   MeSH
• chromatografie kapalinová * metody   MeSH
• lidé MeSH
• oximy * chemie   krev   MeSH
• pyridinové sloučeniny * chemie   krev   MeSH
• reaktivátory cholinesterázy * chemie   MeSH
• tandemová hmotnostní spektrometrie * metody   MeSH
• Check Tag
• lidé MeSH

Reactivation effects of K203 and currently available oximes (obidoxime, HI-6) in combination with atropine on acetylcholinesterase activities in the brain parts of rats poisoned with tabun were studied. The activity was determined by quantitative histochemical and biochemical methods correlating between them very well. The tabun-induced changes in acetylcholinsterase activity as well as in reactivation potency of reactivators used were different in various parts of the brain. Pontomedullar area seems to be important for observed changes following tabun intoxication and its treatment. From the oximes studied, the reactivation effect of K203 was comparable with obidoxime; HI-6 was ineffective. Combination of bio- and histochemical methods allow fine differentiation among the action of different oximes following tabun poisoning.

• MeSH
• chemické bojové látky otrava   MeSH
• cholinesterasy metabolismus   MeSH
• krysa rodu rattus MeSH
• mapování mozku MeSH
• molekulární struktura MeSH
• mozek účinky léků   enzymologie   patologie   MeSH
• neurotoxické syndromy enzymologie   patologie   prevence a kontrola   MeSH
• organofosfáty otrava   MeSH
• oximy aplikace a dávkování   chemie   farmakologie   terapeutické užití   MeSH
• potkani Wistar MeSH
• pyridinové sloučeniny aplikace a dávkování   chemie   farmakologie   terapeutické užití   MeSH
• reaktivátory cholinesterázy aplikace a dávkování   chemie   farmakologie   terapeutické užití   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH