warm spring
Dotaz
Zobrazit nápovědu
Fungi are considered to be the most resilient and economically important microbial community that can easily survive and optimally grow under a wide range of growth conditions. Thermophilic fungi from the geothermal sources have been less pondered upon and lie unexplored. Here, a microbiome approach was conducted to understand the concealed world of the environmental mycobiota from the two hot springs of North Sikkim district located in North-east India. The solfataric muds from the hot springs were analyzed. In both the samples, on the basis of genus level classification, genus Fusarium had the highest abundance followed by Colletotrichum, Pochonia, Pyricularia, Neurospora, etc. Analyzing the predicted genes, the functional proteins of New Yume Samdung mycobiome were found to be dominated by the genera Fusarium (22%), Trichoderma (12%), and Aspergillus (11%), whereas in the case of Old Yume Samdung, it was dominated by the genera Aspergillus (11%), Saccharomyces (6%), and Fusarium (5%). Interestingly, in the studied mycobiome, environmental yeasts were also detected. From the functional metagenomics, sulfate adenylatetransferase (SAT) proteins for sulfur assimilation were found in some of the fungal reads. Toxin protein reads such as AM-toxin biosynthesis proteins, AF-toxin biosynthesis proteins, Gliotoxin biosynthesis proteins, and aflatoxin biosynthesis proteins were detected in the mycobiomes.
- MeSH
- biodiverzita * MeSH
- horké prameny * mikrobiologie MeSH
- houby * fyziologie MeSH
- metagenomika MeSH
- mykobiom * fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Sikkim MeSH
Genotypic and morphological diversity of cyanobacteria in the Rupite hot spring (Bulgaria) was investigated by means of optical microscopy, cultivation, single-cell PCR, and 16S rRNA gene amplicon sequencing. Altogether, 34 sites were investigated along the 71-39 °C temperature gradient. Analysis of samples from eight representative sites shown that Illumina, optical microscopy, and Roche 454 identified 72, 45 and 19% respective occurrences of all cumulatively present taxa. Optical microscopy failed to detect species of minor occurrence; whereas, amplicon sequencing technologies suffered from failed primer annealing and the presence of species with extensive extracellular polysaccharides production. Amplicon sequencing of the 16S rRNA gene V5-V6 region performed by Illumina identified the cyanobacteria most reliably to the generic level. Nevertheless, only the combined use of optical microscopy, cultivation and sequencing methods allowed for reliable estimate of the cyanobacterial diversity. Here, we show that Rupite hot-spring system hosts one of the richest cyanobacterial flora reported from a single site above 50 °C. Chlorogloeopsis sp. was the most abundant at the highest temperature (68 °C), followed by Leptolyngbya boryana, Thermoleptolyngbya albertanoae, Synechococcus bigranulatus, Oculatella sp., and Desertifilum sp. thriving above 60 °C, while Leptolyngbya geysericola, Geitlerinema splendidum, and Cyanobacterium aponinum were found above 50 °C.
Post-diapause, overwintered adults of the true bug Pyrrhocoris apterus L. (Heteroptera, Pyrrhocoridae) form conspicuous aggregations at warm spots in early spring. Using a combination of laboratory experiments and field observations, we assessed the influence of this behaviour on the seasonal timing of reproduction. In the laboratory, post-diapause pairs mated after an accumulation of 80° days (dd), and females started to lay eggs after an additional 80 dd (considering 10.3 °C as a lower temperature threshold for post-diapause development). In the field, however, females mated as early as late March (based on data from four seasons), which corresponded to the accumulation of <15 dd (based on summing the temperatures recorded at a meteorology station). Such a 'discrepancy' between laboratory and field data is explained by thermoregulation. Aggregated adults bask in sunny spots, thereby increasing their body temperatures to ~25 °C, which is 7-16 °C above the temperature of the surrounding ground. This thermal excess speeds up their post-diapause development and enables early mating and oviposition. As a result, behavioural thermoregulation hastens the seasonal start of reproduction in P. apterus by > 1 month.
- MeSH
- chování zvířat * MeSH
- Heteroptera fyziologie MeSH
- roční období MeSH
- rozmnožování fyziologie MeSH
- teplota MeSH
- termoregulace * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Long-term phenological data have been crucial at documenting the effects of climate change in organisms. However, in most animal taxa, time series length seldom exceeds 35 years. Therefore, we have limited evidence on animal responses to climate prior to the recent warm period. To fill in this gap, we present time series of mean first arrival dates to Central Europe for 13 bird species spanning 183 years (1828-2010). We found a uniform trend of arrival dates advancing in the most recent decades (since the late 1970s). Interestingly, birds were arriving earlier during the cooler early part of the nineteenth century than in the recent warm period. Temperature sensitivity was slightly stronger in the warmest 30-year period (-1.70 ± SD 0.47 day °C-1) than in the coldest period (-1.42 ± SD 0.89 day °C-1); however, the difference was not statistically significant. In the most recent decades, the temperature sensitivity of both short- and long-distance migrants significantly increased. Our results demonstrate how centennial time series can provide a much more comprehensive perspective on avian responses to climate change.
- MeSH
- migrace zvířat * MeSH
- ptáci fyziologie MeSH
- roční období MeSH
- teplota MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Late-spring frosts (LSFs) affect the performance of plants and animals across the world's temperate and boreal zones, but despite their ecological and economic impact on agriculture and forestry, the geographic distribution and evolutionary impact of these frost events are poorly understood. Here, we analyze LSFs between 1959 and 2017 and the resistance strategies of Northern Hemisphere woody species to infer trees' adaptations for minimizing frost damage to their leaves and to forecast forest vulnerability under the ongoing changes in frost frequencies. Trait values on leaf-out and leaf-freezing resistance come from up to 1,500 temperate and boreal woody species cultivated in common gardens. We find that areas in which LSFs are common, such as eastern North America, harbor tree species with cautious (late-leafing) leaf-out strategies. Areas in which LSFs used to be unlikely, such as broad-leaved forests and shrublands in Europe and Asia, instead harbor opportunistic tree species (quickly reacting to warming air temperatures). LSFs in the latter regions are currently increasing, and given species' innate resistance strategies, we estimate that ∼35% of the European and ∼26% of the Asian temperate forest area, but only ∼10% of the North American, will experience increasing late-frost damage in the future. Our findings reveal region-specific changes in the spring-frost risk that can inform decision-making in land management, forestry, agriculture, and insurance policy.
- MeSH
- časoprostorová analýza MeSH
- fenotyp MeSH
- klimatické změny * MeSH
- lesy MeSH
- listy rostlin růst a vývoj MeSH
- nízká teplota * MeSH
- roční období * MeSH
- stromy růst a vývoj MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Asie MeSH
- Evropa MeSH
- Severní Amerika MeSH
Microbial mats in hot springs form a dynamic ecosystem and support the growth of diverse communities with broad-ranging metabolic capacity. In this study, we used 16S rRNA gene amplicon sequencing to analyse microbial communities in mat samples from two hot springs in Al Aridhah, Saudi Arabia. Putative metabolic pathways of the microbial communities were identified using phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt). Filamentous anoxygenic phototrophic bacteria associated with phylum Chloroflexi were abundant (> 50 %) in both hot springs at 48 °C. Chloroflexi were mainly represented by taxa Chloroflexus followed by Roseiflexus. Cyanobacteria of genus Arthrospira constituted 3.4 % of microbial mats. Heterotrophic microorganisms were mainly represented by Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Archaea were detected at a lower relative abundance (< 1 %). Metabolic pathways associated with membrane transport, carbon fixation, methane metabolism, amino acid biosynthesis, and degradation of aromatic compounds were commonly found in microbial mats of both hot springs. In addition, pathways for production of secondary metabolites and antimicrobial compounds were predicted to be present in microbial mats. In conclusion, microbial communities in the hot springs of Al Aridhah were composed of diverse bacteria, with taxa of Chloroflexus being dominant.
- MeSH
- Bacteria klasifikace MeSH
- biodiverzita * MeSH
- fylogeneze * MeSH
- horké prameny mikrobiologie MeSH
- metabolické sítě a dráhy MeSH
- RNA ribozomální 16S genetika MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Saudská Arábie MeSH
