This prospective study sought to evaluate the healing quality of implanted ultraporous β-tricalcium phosphate sown with expanded autologous mesenchymal stromal cells (MSCs) into femoral defects during revision hip arthroplasty. A total of 37 osseous defects in 37 patients were treated and evaluated concerning bone regeneration. Nineteen subjects received β-tricalcium phosphate graft material serving as a carrier of expanded autologous MSCs (the trial group A), nine subjects received β-tricalcium phosphate graft material only (the study group B) and nine subjects received cancellous allografts only (the control group C). Clinical and radiographic evaluations were scheduled at 6 weeks, 3, 6, and 12 months post-operatively, and performed at the most recent visit as well. All observed complications were recorded during follow-up to assess the use of an ultraporous β-tricalcium phosphate synthetic graft material combined with expanded MSCs in bone defect repair. The resulting data from participants with accomplished follow-up were processed and statistically evaluated with a Freeman-Halton modification of the Fischer's exact test, a P < 0.05 value was considered to be significant. Whereas no significant difference was observed between the trial group A with β-tricalcium phosphate synthetic graft material serving as a carrier of expanded autologous MSCs and control group C with cancellous impaction allografting in terms of the bone defect healing, significant differences were documented between the study group B with β-tricalcium phosphate graft material only and control group C. Regarding adverse effects, six serious events were recorded during the clinical trial with no causal relationship to the cell product. β-tricalcium phosphate synthetic graft material serving as a carrier of expanded autologous MSCs appears safe and promotes the healing of bone defects in a jeopardized and/or impaired microenvironment. This clinical trial was registered at the EU Clinical Trials Register before patient recruitment (Registration number: EudraCT number 2012-005599-33; Date of registration: 2013-02-04).
- MeSH
- dospělí MeSH
- femur cytologie zranění fyziologie chirurgie MeSH
- fosforečnany vápenaté chemie terapeutické užití MeSH
- kostní náhrady chemie terapeutické užití MeSH
- lidé středního věku MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie MeSH
- regenerace kostí * MeSH
- senioři MeSH
- tkáňové podpůrné struktury chemie MeSH
- transplantace mezenchymálních kmenových buněk metody MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky, fáze II MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
The goal of our study is to design BaTiO3 ferroelectric layers that will cover metal implants and provide improved osseointegration. We synthesized ferroelectric BaTiO3 layers on Pt/fused silica substrates, and we studied their physical and bio-properties. BaTiO3 and Pt layers were prepared using KrF excimer laser ablation at substrate temperature Ts in the range from 200°C to 750°C in vacuum or under oxygen pressure of 10 Pa, 15 Pa, and 20 Pa. The BaTiO3/Pt and Pt layers adhered well to the substrates. BaTiO3 films of crystallite size 60-140 nm were fabricated. Ferroelectric loops were measured and ferroelectricity was also confirmed using Raman scattering measurements. Results of atomic force microscopy topology and the X-ray diffraction structure of the BaTiO3/Pt/fused silica multilayers are presented. The adhesion, viability, growth, and osteogenic differentiation of human osteoblast-like Saos-2 cells were also studied. On days 1, 3, and 7 after seeding, the lowest cell numbers were found on non-ferroelectric BaTiO3, while the values on ferroelectric BaTiO3, on non-annealed and annealed Pt interlayers, and on the control tissue culture polystyrene dishes and microscopic glass slides were similar, and were usually significantly higher than on non-ferroelectric BaTiO3. A similar trend was observed for the intensity of the fluorescence of alkaline phosphatase, a medium-term marker of osteogenic differentiation, and of osteocalcin, a late marker of osteogenic differentiation. At the same time, the cell viability, tested on day 1 after seeding, was very high on all tested samples, reaching 93-99%. Ferroelectric BaTiO3 films deposited on metallic bone implants through a Pt interlayer can therefore markedly improve the osseointegration of these implants in comparison with non-ferroelectric BaTiO3 films.
- MeSH
- buněčné linie MeSH
- elektřina MeSH
- kostní náhrady chemie MeSH
- lasery MeSH
- lidé MeSH
- nanočástice chemie ultrastruktura MeSH
- osteoblasty cytologie MeSH
- osteogeneze MeSH
- oxid křemičitý chemie MeSH
- platina chemie MeSH
- protézy a implantáty MeSH
- sloučeniny barya chemie MeSH
- titan chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Klíčová slova
- easy-graft,
- MeSH
- augmentace alveolárního výběžku * metody MeSH
- biokompatibilní potahované materiály * MeSH
- fosforečnany vápenaté MeSH
- injekce MeSH
- kostní náhrady * farmakologie chemie terapeutické užití MeSH
- lidé MeSH
- mandibula MeSH
- maxila MeSH
- polyethylenglykoly MeSH
- resorpce alveolární kosti chirurgie MeSH
- stomatochirurgické výkony MeSH
- Check Tag
- lidé MeSH
The aim was to construct a composite structure for bone tissue substitute on the basis of a degradable composite of an organic nanofiber carrier and an inorganic matrix in 3D, and to achieve subsequent colonisation by differentiated human mesenchymal stem cells (hMSC) towards osteocytes. We developed an active bone tissue substitute using nanofiber technology for a polycaprolactone (PCL) scaffold with the addition of hydroxyapatite and the colonisation of both components with hMSC with the ability of differentiation towards osteocytes. The constructed composition included the components necessary for bone healing (inorganic and cellular) and it also forms a spatially-oriented 3D structure. We used polycaprolactone Mw 70,000 with electrostatic spinning for the formation of nanofibers using a modified NanospiderTM method. For the inorganic component we used orthophosphate-calcium silicate with a crystal size of 1-2 mm which the nanofiber membrane was coated with. Both components were connected together with a tissue adhesive based of fibrin glue. Cultivated hMSC cells at a concentration of 1.2 × 104/cm2 were multiplied in vitro and then cultivated in the expansion medium. HMSC overgrew both the PCL membrane and the Si-CaP crystals. After colonisation with cultivated cells, this composite 3D structure can serve as a three-dimensional bone tissue replacement.
- Klíčová slova
- polykaprolakton,
- MeSH
- hydroxyapatity MeSH
- kostní náhrady * chemie klasifikace MeSH
- lidé MeSH
- mezenchymální kmenové buňky MeSH
- nanovlákna MeSH
- techniky in vitro MeSH
- vstřebatelné implantáty MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
BACKGROUND: Nanofibrous scaffolds loaded with bioactive nanoparticles are promising materials for bone tissue engineering. METHODS: In this study, composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA) and diamond nanoparticles were fabricated by an electrospinning technique. PLGA was dissolved in a mixture of methylene chloride and dimethyl formamide (2:3) at a concentration of 2.3 wt%, and nanodiamond (ND) powder was added at a concentration of 0.7 wt% (about 23 wt% in dry PLGA). RESULTS: In the composite scaffolds, the ND particles were either arranged like beads in the central part of the fibers or formed clusters protruding from the fibers. In the PLGA-ND membranes, the fibers were thicker (diameter 270 ± 9 nm) than in pure PLGA meshes (diameter 218 ± 4 nm), but the areas of pores among these fibers were smaller than in pure PLGA samples (0.46 ± 0.02 μm(2) versus 1.28 ± 0.09 μm(2) in pure PLGA samples). The PLGA-ND membranes showed higher mechanical resistance, as demonstrated by rupture tests of load and deflection of rupture probe at failure. Both types of membranes enabled the attachment, spreading, and subsequent proliferation of human osteoblast-like MG-63 cells to a similar extent, although these values were usually lower than on polystyrene dishes. Nevertheless, the cells on both types of membranes were polygonal or spindle-like in shape, and were distributed homogeneously on the samples. From days 1-7 after seeding, their number rose continuously, and at the end of the experiment, these cells were able to create a confluent layer. At the same time, the cell viability, evaluated by a LIVE/DEAD viability/cytotoxicity kit, ranged from 92% to 97% on both types of membranes. In addition, on PLGA-ND membranes, the cells formed well developed talin-containing focal adhesion plaques. As estimated by the determination of tumor necrosis factor-alpha levels in the culture medium and concentration of intercellular adhesion molecule-1, MG-63 cells, and RAW 264.7 macrophages on these membranes did not show considerable inflammatory activity. CONCLUSION: This study shows that nanofibrous PLGA membranes loaded with diamond nanoparticles have interesting potential for use in bone tissue engineering.
- MeSH
- buněčná adheze MeSH
- buněčné linie MeSH
- diamant chemie MeSH
- kostní náhrady chemie MeSH
- kyselina mléčná chemie MeSH
- kyselina polyglykolová chemie MeSH
- lidé MeSH
- mikrofilamenta metabolismus MeSH
- mikroskopie elektronová rastrovací MeSH
- myši MeSH
- nanočástice chemie ultrastruktura MeSH
- nanomedicína MeSH
- nanovlákna chemie ultrastruktura MeSH
- osteoblasty cytologie imunologie fyziologie MeSH
- proliferace buněk MeSH
- testování materiálů MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury chemie MeSH
- transmisní elektronová mikroskopie MeSH
- viabilita buněk MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cílem kasuistického sdělení je demonstrovat a biomechanicky vysvětlit remodelaci biomateriálu (hydroxiapatit s křemíkovými ionty, Si-CAP), který byl použit k náhradě kostních defektů vnitřní a zevní kortikalis diafýzy femuru u dívky s achondroplazií při jednodobé prolongaci o 24 mm. Zkrat levého femuru vznikl při prolongaci jedno rovinovým zevním fixátorem Wagner střední v důsledku iatrogenní poruchy remodelace kostního regenerátu. Na obrázcích je dokumentován fenotyp a proporcionalita probandky, výsledek více etapové prolongační terapie a vyrovnání délky dolních končetin (DK) jednodobou prolongací levého femuru. Na základě biomechanických zákonitostí byla vysvětlena remodelace v mediální a laterální kortikalis femuru ovlivněná stíněním tuhé laterální dlahy za 10 měsíců po jednodobé prolongaci. Retrospektivně byla zhodnocena z RTG snímků ve dvou projekcích geometrie svalku (tzv. callus diameter ratio – CDR) při a po prodlužování obou femurů zevním jedno rovinovým fixátorem Wagner střední a upozorněno na závažnou poruchu remodelace svalku femuru po přerušení (osteotomii) distrakčního svalku z důvodu jeho předčasného srůstu.
The aim of our work is to demonstrate and biomechnically explain remodelling of a biomaterial (a novel synthetic, porous, silicate-substituted calcium phosphate – Si-CAP / Actifuse/) which was used for reparation of cortical femoral defects of diaphysis during one stage 24 mm lengthening at a girl with achondroplasia. The shortening of the left femur was caused by a complication of unilateral lengthening (Wagner device, medium- -length) and consequent lesion of bone remodelling. On figures, there are depicted a phenotype and proportionality of a female proband, result of lengthening treatment and equalization of leg length by one stage femoral lengthening. On basis of biomechanical relations, remodelling of medial and lateral femoral corticalis (10 months after one stage lengthening) under a rigid lateral plate shielding was explained The geometry of bone reclaim (so-called callus diameter ratio – CDR) during and after lengthening of both femurs by unilateral Wagner device (medium-length) was retrospectivelly evaluated. The authors alerted to severe failure of callus remodelling after osteotomy of bone reclaim due to its premature unite.
- Klíčová slova
- callus diameter ratio – CDR, prodlužování dolních končetin, náhradní kostní štěpy, hydroxiapatit s křemíkovými ionty, laterální dlaha a remodelace femuru, soustava „kost – dlaha“, soustava „kost – dlaha“,
- MeSH
- achondroplazie chirurgie terapie MeSH
- diafýzy anatomie a histologie patofyziologie MeSH
- dítě MeSH
- femur chirurgie MeSH
- hydroxyapatit terapeutické užití MeSH
- kostní náhrady chemie terapeutické užití MeSH
- lidé MeSH
- prodloužení kosti metody MeSH
- remodelace kosti MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
This review briefly outlines the history and possibilities of bone reconstruction using various types of artificial materials, which allow interaction with cells only on the surface of the implant or enable ingrowth of cells inside the material. Information is also provided on the most important properties of bone cells taking part in bone tissue development, and on diseases and regeneration. The most common cell types used for testing cell-material interaction in vitro are listed, and the most commonly used approaches to this testing are also mentioned. A considerable part of this review is dedicated to the physical and chemical properties of the material surface, which are decisive for the cell-material interaction, and also to modifications to the surface of the material aimed at integrating it better with the surrounding bone tissue. Special attention is paid to the effects of nanoscale and microscale surface roughness on cell behaviour, to material surface patterning, which allows regionally-selective adhesion and growth of cells, and also to the surface chemistry. In addition, coating the materials with bioactive layers is examined, particularly those created by deposition of fullerenes, hybrid metal-fullerene composites, carbon nanotubes, nanocrystalline diamond films, diamond-like carbon, and nanocomposite hydrocarbon plasma polymer films enriched with metals.
- MeSH
- buněčná adheze MeSH
- buněčná diferenciace MeSH
- kostní náhrady chemie MeSH
- lidé MeSH
- nanokompozity MeSH
- osteoblasty cytologie fyziologie MeSH
- povrchové vlastnosti MeSH
- proliferace buněk MeSH
- protézy a implantáty MeSH
- testování materiálů MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Composite materials based on a polyamide fabric (aramid) and a polydymethylsiloxane (PDMS) matrix were designed for application in bone surgery. In order to increase the bioactivity, 2, 5, 10, 15, 20, and 25 vol.% of nano/micro hydroxyapatite (HA) and tricalcium phosphate (TCP) were added. We studied the effect of the additives on the biocompatibility of the composite. It appears that nano additives have a more favorable effect on mechanical properties than microparticles. 15 vol.% of nano hydroxyapatite additive is an optimum amount for final application of the composites as substitutes for bone tissue: in this case both the mechanical properties and the biological properties are optimized without distinct changes in the inner structure of the composite.
- MeSH
- apatity MeSH
- dimethylpolysiloxany MeSH
- fosforečnany vápenaté MeSH
- hydroxyapatit MeSH
- kosti a kostní tkáň chirurgie MeSH
- kostní náhrady chemie normy MeSH
- králíci MeSH
- krevní plazma MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- mikrotechnologie MeSH
- nanostruktury MeSH
- nylony MeSH
- osteointegrace fyziologie MeSH
- polymery MeSH
- povrchové vlastnosti MeSH
- pružnost MeSH
- software MeSH
- testování materiálů metody MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This article reviews the development of artificial bone substitutes from their older single-phase forms to novel multi-phase composites, mimicking the composition and architecture of natural bone tissue. The new generation of bone implants should be bioactive, i.e. they should induce the desired cellular responses, leading to integration of the material into the natural tissue and stimulating self-healing processes. Therefore, the first part of the review explains the common principles of the cellmaterial interaction and summarizes the strategies how to improve the biocompatibility and bioactivity of the materials by modifying the physico-chemical properties of the material surface, such as surface chemistry, wettability, electrical charge, rigidity, microroughness and especially nanoroughness. The latter has been shown to stimulate preferentially the growth of osteoblasts in comparison with other competitive cell types, such as fibroblasts, which could prevent fibrous tissue formation upon implantation. The second more specialized part of the review deals with materials suitable for bone contact and substitution, particularly novel polymer-based composites reinforced with fibres or inorganic particles and containing bioactive components, such as crystals of hydroxyapatite or other calcium phosphates, synthetic ligands for cell adhesion receptors or growth factors. Moreover, if they are degradable, they can be gradually replaced with a regenerating tissue.
- MeSH
- biokompatibilní materiály MeSH
- financování organizované MeSH
- kostní náhrady chemie terapeutické užití MeSH
- lidé MeSH
- osteoblasty fyziologie MeSH
- osteogeneze MeSH
- osteointegrace MeSH
- povrchové vlastnosti MeSH
- protézy - design MeSH
- tkáňové inženýrství MeSH
- transplantace kostí přístrojové vybavení MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
