Intestinal ischemic-reperfusion (IR) injury has detrimental effects on both local and distant organs in the body. Betanin is known for its antioxidant properties, and it is found mostly in vegetables. Therefore, the aim of the present study was to test the hypothesis that betanin administration prior intestinal IR, may be beneficial in protecting jejunal mucosa and lung parenchyma against IR damage. Male specific pathogen-free Charles River Wistar rats were used (n = 42). Betanin (50 mg/kg) was administered intraperitoneally 30 min before ischemia of the superior mesenteric artery lasting 1 h, followed by 1, 4 and 24 h of reperfusion. Immunohistochemical as well as histomorphometrical analysis indicated a protective effect of betanin pretreatment on jejunal tissue. Regarding morphometrical analysis betanin significantly (p < 0.01) augments intestinal villus height after 24 of reperfusion comparing to early stages. Betanin application reduced number of mast cells population in early reperfusion periods (p < 0.05). The protective effect of betanin on lung parenchyma, was detected in late reperfusion period (24 h) with improvement of histopathological injury index and morphometric analysis (p < 0.001 for both). The improvement of histopathological injury index (p < 0.001) and morphometric analysis (p < 0.001) during the late reperfusion period, suggests a protective effect of betanin on lung parenchyma. Moreover, suppression of the inflammatory response was mirrored by the reduction of myeloperoxidase (MPO) positive cells within lung parenchyma after 1 and 4 h of reperfusion (p < 0.001). Especially, during the first 4 h of reperfusion after betanin administration, a reduction of 74% of the polymorphonuclear neutrophils infiltration (MPO positive cell population) and of a nearly 46% of active MCs was observed. Upon morphometric examination, the lung histological architecture after 24 h of reperfusion appeared to be almost 100% better following betanin treatment, with 25% thinner interalveolar septa and 20% larger alveolar surface for respiratory gas exchange. The results suggest that betanin pretreatment protects the jejunal mucosa and the lung parenchyma, as well as reduces the inflammatory cell density after intestinal IR injury.
- MeSH
- betakyaniny aplikace a dávkování farmakologie MeSH
- jejunum účinky léků zranění patologie MeSH
- krysa rodu rattus MeSH
- parenterální výživa MeSH
- plíce účinky léků patologie MeSH
- potkani Wistar MeSH
- reperfuzní poškození komplikace MeSH
- zánět farmakoterapie etiologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- Beta vulgaris * imunologie metabolismus MeSH
- betalainy izolace a purifikace metabolismus terapeutické užití MeSH
- diabetes mellitus dietoterapie metabolismus MeSH
- dusičnany izolace a purifikace metabolismus terapeutické užití MeSH
- endoteliální buňky patologie účinky léků MeSH
- experimenty na zvířatech * MeSH
- fenoly izolace a purifikace metabolismus terapeutické užití MeSH
- hypertenze dietoterapie farmakoterapie MeSH
- kardiovaskulární nemoci dietoterapie metabolismus MeSH
- kyselina askorbová terapeutické užití MeSH
- metabolické nemoci etiologie terapie MeSH
- oxid dusnatý izolace a purifikace škodlivé účinky MeSH
- oxidační stres imunologie účinky léků MeSH
- potkani inbrední SHR MeSH
- rostlinné extrakty * imunologie metabolismus terapeutické užití MeSH
- statistika jako téma MeSH
- zánět dietoterapie farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
x
- Klíčová slova
- muscazone, stizolobic acid,
- MeSH
- Amanita * chemie patogenita MeSH
- aminokyseliny dikarboxylové MeSH
- betalainy MeSH
- biologické toxiny MeSH
- halucinace chemicky indukované MeSH
- isoxazoly MeSH
- kyselina ibotenová MeSH
- lidé MeSH
- muscimol MeSH
- muskarin MeSH
- otrava houbami patofyziologie MeSH
- oxazoly MeSH
- polysacharidy MeSH
- vanad MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
- Klíčová slova
- betanin, beeturie,
- MeSH
- Beta vulgaris MeSH
- betakyaniny metabolismus moč MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
Food adulteration remains a great concern for consumers in the developed countries; examples include pro-ducts of red and blue berries as fruit drinks, syrups or jams. Undeclared coloring can mask a lower proportion of fruits in the final product or the use of lower quality raw materials. Black carrot, chokeberry, elderberry, grapes, pomegranate and red beet belong among the fruit/vegetable species used for coloring. The intensity and hue of red or blue color in fruits and vegetables are determined by individual anthocyanins, betalains and carotenoids, characteristic for the given species. The particular profile of anthocyanins, as measured by HPLC method, is recommended to be used for the undeclared coloring detection.
- MeSH
- analýza potravin zákonodárství a právo MeSH
- anthokyaniny * analýza MeSH
- barvicí látky * analýza škodlivé účinky MeSH
- betalainy * analýza MeSH
- chromatografie na tenké vrstvě metody využití MeSH
- elektroforéza kapilární metody využití MeSH
- karotenoidy * analýza MeSH
- lidé MeSH
- potravinářská technologie zákonodárství a právo MeSH
- potravinářské přísady MeSH
- vysokoúčinná kapalinová chromatografie metody využití MeSH
- Check Tag
- lidé MeSH
