- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence genetika MeSH
- Escherichia coli genetika MeSH
- infekce vyvolané Escherichia coli veterinární MeSH
- kloaka mikrobiologie MeSH
- kur domácí mikrobiologie MeSH
- plazmidy genetika MeSH
- proteiny z Escherichia coli genetika MeSH
- transferasy pro jiné substituované fosfátové skupiny genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- dopisy MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Paraguay MeSH
OBJECTIVES: The objective of this study was to investigate the silver gull as an indicator of environmental contamination by salmonellae and carbapenemase-producing Enterobacteriaceae (CPE) in south-east Australia. METHODS: A total of 504 cloacal samples were collected from gull chicks at three nesting colonies in New South Wales, Australia [White Bay (n = 144), Five Islands (n = 200) and Montague Island (n = 160)] and were examined for salmonellae and CPE. Isolates were tested for carbapenemase genes and susceptibility to 14 antibiotics. Clonality was determined by PFGE and MLST. Genetic context and conjugative transfer of the carbapenemase gene were determined. RESULTS: A total of 120 CPE of 10 species, mainly Escherichia coli (n = 85), carrying the gene blaIMP-4, blaIMP-38 or blaIMP-26 were obtained from 80 (40%) gulls from Five Islands. Thirty percent of birds from this colony were colonized by salmonellae. Most isolates contained the gene within a class 1 integron showing a blaIMP-4-qacG-aacA4-catB3 array. The blaIMP gene was carried by conjugative plasmids of variable sizes (80-400 kb) and diverse replicons, including HI2-N (n = 30), HI2 (11), A/C (17), A/C-Y (2), L/M (5), I1 (1) and non-typeable (6). Despite the overall high genetic variability, common clones and plasmid types were shared by different birds and bacterial isolates, respectively. CONCLUSIONS: Our data demonstrate a large-scale transmission of carbapenemase-producing bacteria into wildlife, likely as a result of the feeding habits of the birds at a local waste depot. The isolates from gulls showed significant similarities with clinical isolates from Australia, suggesting the human origin of the isolates. The sources of CPE for gulls on Five Islands should be explored and proper measures applied to stop the transmission into the environment.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální proteiny analýza genetika MeSH
- beta-laktamasy analýza genetika MeSH
- Charadriiformes mikrobiologie MeSH
- Enterobacteriaceae enzymologie izolace a purifikace MeSH
- enterobakteriální infekce epidemiologie mikrobiologie veterinární MeSH
- genotyp MeSH
- kloaka mikrobiologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- molekulární epidemiologie MeSH
- molekulární typizace MeSH
- nemoci ptáků epidemiologie mikrobiologie MeSH
- ostrovy epidemiologie MeSH
- prevalence MeSH
- ptáci MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Nový Jižní Wales epidemiologie MeSH
- ostrovy epidemiologie MeSH
Effects of vertebrate-associated microbiota on physiology and health are of significant interest in current biological research. Most previous studies have focused on host-microbiota interactions in captive-bred mammalian models. These interactions and their outcomes are still relatively understudied, however, in wild populations and non-mammalian taxa. Using deep pyrosequencing, we described the cloacal microbiome (CM) composition in free living barn swallows Hirundo rustica, a long-distance migratory passerine bird. Barn swallow CM was dominated by bacteria of the Actinobacteria, Proteobacteria and Firmicutes phyla. Bacteroidetes, which represent an important proportion of the digestive tract microbiome in many vertebrate species, was relatively rare in barn swallow CM (< 5%). CM composition did not differ between males and females. A significant correlation of CM within breeding pair members is consistent with the hypothesis that cloacal contact during within-pair copulation may promote transfer of bacterial assemblages. This effect on CM composition had a relatively low effect size, however, possibly due to the species' high level of sexual promiscuity.
- MeSH
- bakteriální RNA MeSH
- biodiverzita MeSH
- chov MeSH
- kloaka mikrobiologie MeSH
- metagenom MeSH
- migrace zvířat * MeSH
- mikrobiota * MeSH
- ptáci mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We aimed at Escherichia coli and Enterobacter cloacae isolates resistant to cephalosporins and fluoroquinolones and Salmonella isolates in wild birds in Arctic Svalbard, Norway. Cloacal swabs of little auks (Alle alle, n=215) and samples of faeces of glaucous gulls (Larus hyperboreus, n=15) were examined. Inducible production of AmpC enzyme was detected in E. cloacae KW218 isolate. Sequence analysis of the 1146 bp PCR product of the ampC gene from this isolate revealed 99% sequence homology with the blaACT-14 and blaACT-5 AmpC beta-lactamase genes. Four, respectively six of the identified single nucleotide polymorphisms generated amino acid substitutions in the amino acid chain. As the ampC sequence polymorphism in the investigated E. cloacae strain was identified as unique, we revealed a novel variant of the ampC beta-lactamase gene blaACT-23.
- MeSH
- bakteriální léková rezistence genetika MeSH
- bakteriální proteiny genetika metabolismus MeSH
- beta-laktamasy genetika metabolismus MeSH
- Charadriiformes mikrobiologie MeSH
- DNA primery genetika MeSH
- Enterobacter cloacae enzymologie genetika MeSH
- Escherichia coli genetika MeSH
- jednonukleotidový polymorfismus genetika MeSH
- kloaka mikrobiologie MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce veterinární MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA veterinární MeSH
- substituce aminokyselin genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Norsko MeSH
- Svalbard MeSH
OBJECTIVES: Faecal Escherichia coli strains were isolated from great cormorants (Phalacrocorax carbo) and mallards (Anas platyrhynchos), which are commonly occurring waterbirds in Europe, and studied for resistance to cephalosporins and fluoroquinolones. METHODS: Cloacal swabs or faeces from great cormorants and mallards in Central Europe were cultivated to isolate Escherichia coli strains with extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance (PMQR) genes. RESULTS: Ten ESBL-producing E. coli with the bla(CTX-M-15) or bla(CTX-M-27) gene were isolated from eight great cormorants (1.6%, n = 499). The bla(CTX-M) genes were harboured by plasmids of F and I1 incompatibility groups. CTX-M-27-producing isolates were identified as the epidemiologically important B2-O25b-ST131 clone. No ESBL-producing E. coli was isolated from 305 mallards. Eight E. coli isolates with PMQR genes [six aac(6')-Ib-cr and two qnrS1] were detected in six great cormorants (1.2%). Seventeen strains with qnrS1 were detected in 17 mallards (6%). The PMQR genes were located on plasmids of incompatibility groups F, N or X2. ESBL and PMQR genes were found on conjugative plasmids, enabling the horizontal spread of resistance. CONCLUSIONS: Both great cormorants and mallards can spread epidemiologically important antimicrobial-resistant E. coli isolates to water bodies throughout Europe.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence MeSH
- beta-laktamasy sekrece MeSH
- cefalosporiny farmakologie MeSH
- chinolony farmakologie MeSH
- Escherichia coli enzymologie genetika izolace a purifikace MeSH
- feces mikrobiologie MeSH
- kloaka mikrobiologie MeSH
- plazmidy analýza MeSH
- ptáci mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa MeSH
Individual cloacal swabs of mallards (Anas platyrhynchos) and of herring gulls (Larus argentatus), as well as samples of waterbird feces obtained in 2008 and 2009, were cultivated for Escherichia coli. Isolates of E. coli were tested for susceptibilities to 12 antimicrobial agents by the disk diffusion method. Moreover, the samples were subcultivated on MacConkey agar (MCA) containing cefotaxime (2 mg liter(-1)) to detect E. coli with extended-spectrum beta-lactamase (ESBL) and subsequently on MCA supplemented with ciprofloxacin (0.05 mg liter(-1)) and MCA with nalidixic acid (20 mg liter(-1)) to isolate fluoroquinolone-resistant E. coli. PCR was used to detect specific antibiotic resistance genes. We found 9 E. coli isolates producing ESBL with bla genes: bla(CTX-M-1) (6 isolates), bla(CTX-M-9) plus bla(TEM-1b) (1 isolate), bla(CTX-M-15) plus bla(OXA-1) (1 isolate), and bla(SHV-12) (1 isolate). In the isolate with bla(CTX-M-15), the gene aac(6)-Ib-cr was also detected. The bla genes were harbored by transferable plasmids of the IncN and IncI1 groups. Nine quinolone-resistant E. coli isolates with qnrS genes were found and characterized. The gene qnrS was associated with a Tn3-like transposon on the IncX1 plasmid together with bla(TEM-1) in two isolates. The gene qnrS was also harbored by conjugative plasmids of the IncN and IncX2 groups. Even if populations of wild birds are not directly influenced by antibiotic practice, we have demonstrated that antibiotic-resistant E. coli strains, including strains with various ESBL and qnrS genes, are found in the feces of wild birds on the coast of the Baltic Sea in Poland.
- MeSH
- Anseriformes mikrobiologie MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny MeSH
- bakteriální léková rezistence MeSH
- beta-laktamasy biosyntéza MeSH
- Charadriiformes mikrobiologie MeSH
- Escherichia coli účinky léků enzymologie genetika izolace a purifikace MeSH
- feces mikrobiologie MeSH
- kloaka mikrobiologie MeSH
- kultivační média chemie MeSH
- mikrobiální testy citlivosti MeSH
- plazmidy analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- oceány a moře MeSH
- Polsko MeSH
The susceptibility toward antibiotics was determined by disc and MIC methods in Lactobacillus and, for comparison, in Escherichia coli strains isolated from cloacal swabs of broiler chickens derived from various farms in Slovakia. The occurrence of acquired tetracycline resistance in E. coli and lactobacilli isolated from the same sample was similar. The presence of tet(M), tet(S), tet(L) and ermB genes was demonstrated in lactobacilli while the tet(M) gene was not detected in E. coli.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny MeSH
- bakteriální léková rezistence MeSH
- Escherichia coli izolace a purifikace účinky léků MeSH
- financování organizované MeSH
- kloaka mikrobiologie MeSH
- kur domácí MeSH
- Lactobacillus izolace a purifikace účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- polymerázová řetězová reakce MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Slovenská republika MeSH
In the period between September 2002 and May 2004, a total of 6023 rectal swabs from humans in the Czech Republic were evaluated and 821 Enterococcus spp. strains were isolated. Nine strains (1.1 %) were identified as vancomycin-resistant enterococci (VRE). Two strains were VanA Enterococcus faecium, one strain was VanB Enterococcus faecalis and six strains were VanC Enterococcus casseliflavus. In total, 527 Enterococcus spp. strains were isolated from poultry breeds of which 11 (2.1 %) were VRE. Most (54.5 %) were identified as VanA E. faecium. Cluster analysis of SmaI-generated macrorestriction patterns showed high variability in both human and animal VRE strains and no relatedness between strains from the two sources.
- MeSH
- bakteriální proteiny genetika MeSH
- DNA bakterií genetika MeSH
- DNA fingerprinting MeSH
- drůbež mikrobiologie MeSH
- Enterococcus genetika izolace a purifikace klasifikace účinky léků MeSH
- financování vládou MeSH
- genetická variace MeSH
- grampozitivní bakteriální infekce epidemiologie mikrobiologie MeSH
- kloaka mikrobiologie MeSH
- lidé MeSH
- ligasy tvořící vazby C-O genetika MeSH
- peptidsynthasy genetika MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- přenašečství epidemiologie MeSH
- rektum mikrobiologie MeSH
- restrikční endonukleasy typu II MeSH
- rezistence na vankomycin genetika MeSH
- shluková analýza MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Geografické názvy
- Česká republika MeSH