Morel's disease is a form of abscessing lymphadenitis of sheep and goats caused by Staphylococcus aureus subspecies anaerobius. In Europe and Africa, the disease is linked to S. aureus of multilocus sequence type 1464. In an outbreak recorded in 2015 in a flock of 530 animals in the district of Nymburk, Czech Republic, Europe, the causative agent was cultured and subsequently confirmed by Maldi-TOF. Neither antibiotic therapy nor surgical interventions met any success, although the strain isolated was found to be sensitive to antibiotics used. Vaccination and revaccination with inactivated autogenous vaccine administered subcutaneously was relatively successful. Subsequent multilocus sequence typing revealed the presence of new S. aureus sequence type 3756, different from 1464 in three out of seven genes typed. The isolate thus represents a new sequence type of Staphylococcus aureus ssp. anaerobius which should be considered as a causative agent of Morel's disease.
- Klíčová slova
- Autogenous vaccine, Europe, Morel's disease, S. aureus, Therapy,
- MeSH
- antibakteriální látky terapeutické užití MeSH
- lymfadenitida farmakoterapie mikrobiologie veterinární MeSH
- mikrobiální testy citlivosti MeSH
- multilokusová sekvenční typizace MeSH
- nemoci ovcí farmakoterapie mikrobiologie MeSH
- ovce MeSH
- stafylokokové infekce farmakoterapie mikrobiologie veterinární MeSH
- stafylokokové vakcíny imunologie MeSH
- Staphylococcus aureus klasifikace imunologie izolace a purifikace MeSH
- vakcinace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- antibakteriální látky MeSH
- stafylokokové vakcíny MeSH
The interleukin 17 (IL-17) cytokine and receptor family is central to antimicrobial resistance and inflammation in the lung. Mice lacking IL-17A, IL-17F, or the IL-17RA subunit were compared with wild-type mice for susceptibility to airway inflammation in models of infection and allergy. Signaling through IL-17RA was required for efficient microbial clearance and prevention of allergy; in the absence of IL-17RA, signaling through IL-17RC on epithelial cells, predominantly by IL-17F, significantly exacerbated lower airway Aspergillus or Pseudomonas infection and allergic airway inflammation. In contrast, following infection with the upper respiratory pathogen Staphylococcus aureus, the IL-17F/IL-17RC axis mediated protection. Thus, IL-17A and IL-17F exert distinct biological effects during pulmonary infection; the IL-17F/IL-17RC signaling axis has the potential to significantly worsen pathogen-associated inflammation of the lower respiratory tract in particular, and should be investigated further as a therapeutic target for treating pathological inflammation in the lung.
- Klíčová slova
- ABPA, IL-17F/IL-17RC axis, Th17 immunity, allergy, respiratory infections,
- MeSH
- alergie genetika imunologie mikrobiologie patologie MeSH
- Aspergillus imunologie MeSH
- aspergilóza genetika imunologie mikrobiologie patologie MeSH
- epitelové buňky imunologie mikrobiologie patologie MeSH
- interleukin-17 nedostatek genetika imunologie MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- náchylnost k nemoci MeSH
- plíce imunologie mikrobiologie patologie MeSH
- protein - isoformy nedostatek genetika imunologie MeSH
- pseudomonádové infekce genetika imunologie mikrobiologie patologie MeSH
- Pseudomonas imunologie MeSH
- receptory interleukinu-17 nedostatek genetika imunologie MeSH
- regulace genové exprese MeSH
- respirační sliznice imunologie mikrobiologie patologie MeSH
- signální transdukce MeSH
- stafylokokové infekce genetika imunologie mikrobiologie patologie MeSH
- Staphylococcus aureus imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- Il17ra protein, mouse MeSH Prohlížeč
- interleukin-17 MeSH
- protein - isoformy MeSH
- receptory interleukinu-17 MeSH
The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in 13 strains. In 9 strains, selk occurred together with the sea gene. Out of 74 strains recovered from nasal carriers, 56 (75 %) were found to carry SAg genes, 38 carried egc genes, while selk was detected in 5 strains. The predominant SAg types in both investigated S. aureus populations were egc and selk/sea, but selk gene frequency was significantly higher in the CF-derived strains.
- MeSH
- antigeny bakteriální analýza genetika MeSH
- bakteriální proteiny genetika MeSH
- cystická fibróza komplikace mikrobiologie MeSH
- dítě MeSH
- DNA bakterií genetika MeSH
- enterotoxiny genetika MeSH
- genotyp MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- nosní dutina mikrobiologie MeSH
- novorozenec MeSH
- pacienti MeSH
- předškolní dítě MeSH
- přenašečství mikrobiologie MeSH
- sputum mikrobiologie MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus aureus klasifikace genetika imunologie izolace a purifikace MeSH
- superantigeny analýza genetika MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny bakteriální MeSH
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- enterotoxiny MeSH
- superantigeny MeSH
The influence of Saccharomyces cerevisiae var. vini viable cells on the Staphylococcus aureus-suffering mice was ascertained: (1) effect of S. aureus living cells on antibacterial and antitoxic antibodies, (2) effect of S. aureus on the number of neurons and macroglial cells in different areas of mouse hippocampus, (3) effect of S. cerevisiae var. vini viable cells on the above-mentioned changes of immune and nervous system. Treatment with S. cerevisiae var. vini provokes immune stimulation and change of the total number of macroglial elements in the CA1 field of hippocampus.
- MeSH
- hipokampus patologie MeSH
- histocytochemie MeSH
- mikroglie patologie MeSH
- modely nemocí na zvířatech MeSH
- mozek patologie MeSH
- myši MeSH
- neurony patologie MeSH
- probiotika aplikace a dávkování MeSH
- protilátky bakteriální krev MeSH
- Saccharomyces cerevisiae růst a vývoj MeSH
- stafylokokové infekce imunologie mikrobiologie patologie terapie MeSH
- Staphylococcus aureus imunologie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- protilátky bakteriální MeSH
This study was undertaken to investigate the time course of surface expression of CD14 on neutrophils and macrophages and to determine their association with resolution of inflammatory responses during Staphylococcus aureus and Streptococcus uberis experimental mastitis. Infections of the mammary gland induce a local immune response characterized by an increase in the total counts of CD14+ neutrophils and CD14+ macrophages particularly. On the other hand, resolution is accompanied by an increase in relative counts of CD14+ neutrophils, CD14+ vacuolized macrophages and apoptotic neutrophils. Following the immune reaction of mammary gland against Gram-negative/positive bacteria is very similar. Between the apoptotic and CD14+ neutrophils a high correlation was measured during the whole experimental period (S. aureus: r=0.64; S. uberis: r=0.61; P<0.05). Using anti-CD14 monoclonal antibodies in vitro suggested the involving of the CD14 surface receptor in recognition of apoptotic neutrophils by macrophages.
- MeSH
- antigeny CD14 imunologie MeSH
- apoptóza imunologie MeSH
- fagocytóza imunologie MeSH
- makrofágy imunologie MeSH
- mastitida skotu imunologie mikrobiologie MeSH
- neutrofily imunologie MeSH
- průtoková cytometrie veterinární MeSH
- skot MeSH
- stafylokokové infekce imunologie mikrobiologie veterinární MeSH
- Staphylococcus aureus imunologie MeSH
- Streptococcus imunologie MeSH
- streptokokové infekce imunologie mikrobiologie veterinární MeSH
- transmisní elektronová mikroskopie veterinární MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD14 MeSH
UNLABELLED: Procalcitonin (PCT) is a highly selective and specific marker for early diagnosis of sepsis. There is enough information confirming that procalcitonin should be considered as an important mediator in the pathophysiology of sepsis. The aim of our work was to evaluate the effect of recombinant human procalcitonin (rhPCT) on phagocytic and candidacidal activity of polymorphonuclear leukocytes (PMNL) in blood, as well as on killing mechanisms of fresh serum and blood against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Our results show that rhPCT dose dependently decreased both phagocytic and candidacidal activity of polymorphonuclear leukocytes (p < 0.001). RhPCT inhibited also the microbicidal activity of both serum and blood on E. coli that was found by inoculation of suspension on culture plates. We found that rhPCT supported the E. coli colony count increase during the incubation in the presence of both serum and blood. The effect of rhPCT on the S. aureus colony count increase was not statistically significant. CONCLUSION: Our results indicate a suppressive effect of rhPCT on phagocytic and microbicidal activity of polymorphonuclear leucocytes.
- MeSH
- baktericidní aktivita krve účinky léků MeSH
- Candida albicans imunologie MeSH
- Escherichia coli imunologie MeSH
- fagocytóza účinky léků MeSH
- kalcitonin farmakologie MeSH
- lidé MeSH
- neutrofily účinky léků fyziologie MeSH
- peptid spojený s genem pro kalcitonin MeSH
- proteinové prekurzory farmakologie MeSH
- rekombinantní proteiny farmakologie MeSH
- Staphylococcus aureus imunologie MeSH
- techniky in vitro MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- CALCA protein, human MeSH Prohlížeč
- kalcitonin MeSH
- peptid spojený s genem pro kalcitonin MeSH
- proteinové prekurzory MeSH
- rekombinantní proteiny MeSH
The thyroid hormones may exert an important effect on neutrophil functions which play a key role in the defence against microbial pathogens. Therefore investigations of the biological functions of neutrophils in peripheral blood of 80 women with hyperthyroidism were performed. The following functions of neutrophils were examined: nitroblue tetrazolium (NBT) reduction ability, phagocytosis, adherence to fibers as well as spontaneous migration of leukocytes and activity of lysozyme in serum. The total number of leukocytes and neutrophils was also determined. The results were compared with those in the control group consisting of 40 healthy women. The data show that in patients with hyperthyroidism, the number of leukocytes and neutrophils was decreased (respectively 4.90 x 10(9) +/- 1.20, 2.70 x 10(9) +/- 0.54 in patients, and 5.40 x 10(9) +/- 1.30, 3.00 x 10(9) +/- 0.74 in the control subjects), index of spontaneous reduction NBT was increased (17.1% +/- 4.5 in patients and 11.1% +/- 4.5 in the control group), index of phagocytosing cells was decreased (63.0% +/- 16.0, 80.3% +/- 14.0 in patients and 72.2% +/- 12.1, 87.4% +/- 12.0 in the control group), an increase in adherence (71% +/- 17 in patients and 58% +/- 10 in the control subjects), and a decrease in the lysozyme activity in undiluted and diluted serum was found (185 IU/ml +/- 85,209 IU/ml +/- 78 in patients and 284 IU/ml +/- 84,352 IU/ml +/- 140 in the control group).
- MeSH
- aktivace neutrofilů MeSH
- buněčná adheze MeSH
- dospělí MeSH
- fagocytóza MeSH
- hypertyreóza imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- muramidasa krev metabolismus MeSH
- neutrofily imunologie MeSH
- počet leukocytů MeSH
- pohyb buněk MeSH
- Staphylococcus aureus imunologie MeSH
- studie případů a kontrol MeSH
- techniky in vitro MeSH
- tetrazoliová modř metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- muramidasa MeSH
- tetrazoliová modř MeSH
Human mononuclear leukocytes kill Staphylococcus aureus cells in vitro. The killing of the bacteria takes place even in the absence of antibodies. The presence of antibodies (in an autologous inactivated serum) usually enhances the antibacterial activity of mononuclear leukocytes. In some cases, however, this activity is markedly decreased by the serum, probably depending of the spectrum of antibodies contained in the serum. The antibacterial activity of mononuclear leukocytes is mostly due to monocytes because their depletion causes substantial drop or the activity disappearance. We failed to demonstrate in the case of S. aureus the antibacterial cytotoxicity of T lymphocytes described by some authors dealing with Gram-negative bacteria. Large differences in the structure of the bacterial cell wall underlie apparently the different sensitivity of G+ and G- bacteria to some protective mechanisms of the host. In the antibacterial assay against S. aureus, electron microscopy revealed a maximal activation of monocytes which phagocytized the bacteria although extracellular killing is not excluded. Electronoptical findings point also to a possible participation of NK cells in the antibacterial cytotoxicity against S. aureus.
- MeSH
- baktericidní aktivita krve MeSH
- buněčná cytotoxicita závislá na protilátkách MeSH
- buněčná stěna imunologie MeSH
- cytotoxicita imunologická MeSH
- dospělí MeSH
- elektronová mikroskopie MeSH
- leukocyty mononukleární imunologie ultrastruktura MeSH
- lidé MeSH
- Staphylococcus aureus imunologie patogenita MeSH
- techniky in vitro MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
To objectivize potential modulatory effects of polybacterial lysate Olimunostim on cellular immunity, the T-lymphocyte subpopulations and lymphocyte reactivity to mitogens were followed up in 10 healthy volunteers subjected to Olimunostim application and parallelly in 6 healthy individuals receiving placebo. The preparation, which was applied perorally in seven consecutive daily doses, had no effects on T-lymphocytes and their subpopulations and exerted only a limited influence on lymphocyte reactivity to mitogens. Both the moderate suppression of proliferative response to Con A and PWM, observed after the application of the three doses of Olimunostim, and the insignificantly enhanced response to PHA and Con A, revealed shortly after the last dose was given, were only of transient character and in comparison with the control group were not proved to be statistically significant. To explain the discordance of these results with the recently reported modulatory effects of Olimunostim on murine lymphocytes it is suggested, that the performed tests using the peripheral blood lymphocytes cannot mirror the processes developing in the diseased tissues. With respect to this presumption and also to the observations of enhanced immunomodulatory effect of Olimunostim on terrain altered by infection, it is proposed to investigate cellular immune parameters in specific disease sites of Olimunostim treated patients suffering from recurrent infections.
- MeSH
- adjuvancia imunologická farmakologie MeSH
- aktivace lymfocytů účinky léků MeSH
- buněčná imunita účinky léků MeSH
- dospělí MeSH
- Klebsiella pneumoniae imunologie MeSH
- lidé MeSH
- placeba MeSH
- Propionibacterium acnes imunologie MeSH
- referenční hodnoty MeSH
- Staphylococcus aureus imunologie MeSH
- T-lymfocyty účinky léků imunologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky kontrolované MeSH
- klinické zkoušky MeSH
- srovnávací studie MeSH
- Názvy látek
- adjuvancia imunologická MeSH
- placeba MeSH
To objectivize possible immunomodulatory effects of polybacterial lysate Olimunostim (P. acnes, K. pneumoniae, S. aureus), splenic lymphocyte proliferation and T-lymphocyte subsets were followed-up in Balb/c mice administered perorally the lysate or saline (controls). The enhanced spontaneous lymphocyte proliferation observed at the beginning of Olimunostim application preceded a gradual increase of lymphocyte reactivity to T-mitogens reaching the maximum five days after the administration of the last dose and returning back to the control levels ten days afterwards. This stimulation of lymphocyte proliferation was accompanied by Olimunostim induced increase of CD4+ splenic lymphocytes being most pronounced five days after the end of immunomodulation and later returning to the initial values. The last experiment revealed an enhanced response of the in vivo primed lymphocytes after the re-exposure to Olimunostim in vitro. It is concluded that mostly nonspecific activation mechanisms, plausibly also parallelly induced specific immunity, are involved in Olimunostim modulatory effects on the cellular immune response.
- MeSH
- adjuvancia imunologická farmakologie MeSH
- aktivace lymfocytů účinky léků MeSH
- Klebsiella pneumoniae imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- Propionibacterium acnes imunologie MeSH
- slezina imunologie MeSH
- Staphylococcus aureus imunologie MeSH
- T-lymfocyty - podskupiny účinky léků imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adjuvancia imunologická MeSH
