Nejvíce citovaný článek - PubMed ID 12630313
PCR-based methods for identification of Enterococcus species
Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.
- MeSH
- bakteriální proteiny analýza MeSH
- bakteriologické techniky metody MeSH
- DNA bakterií chemie genetika MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- kozy MeSH
- Limosilactobacillus reuteri klasifikace genetika izolace a purifikace fyziologie MeSH
- mezerníky ribozomální DNA chemie genetika MeSH
- ovce MeSH
- polymerázová řetězová reakce MeSH
- reprodukovatelnost výsledků MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- žaludeční sliznice mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- srovnávací studie MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- mezerníky ribozomální DNA MeSH
One-hundred sixty Escherichia coli isolates obtained from piglets with diarrhea from different parts of Slovakia were examined for the presence of genes coding for F4, F5, F6 and F41 fimbrial adhesins, and hemolytic activity. According to polymerase chain reaction tests 74 (46%) E. coli isolates were positive for primers that detected genes coding for fimbrial adhesins. Of these 74 isolates, 64 were positive for genes encoding for F4+, four for F5+, five for F6+, and one for both F41+ and F5+ adhesins.
- MeSH
- adheziny Escherichia coli genetika MeSH
- antigeny bakteriální genetika MeSH
- bakteriální adheziny genetika MeSH
- bakteriální toxiny biosyntéza MeSH
- DNA bakterií analýza genetika MeSH
- Escherichia coli genetika izolace a purifikace MeSH
- genotyp MeSH
- hemolyziny biosyntéza MeSH
- infekce vyvolané Escherichia coli mikrobiologie veterinární MeSH
- nemoci prasat mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- prasata mikrobiologie MeSH
- proteiny z Escherichia coli genetika MeSH
- průjem mikrobiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika MeSH
- Názvy látek
- adheziny Escherichia coli MeSH
- antigeny bakteriální MeSH
- bakteriální adheziny MeSH
- bakteriální toxiny MeSH
- DNA bakterií MeSH
- F41 antigen, E coli MeSH Prohlížeč
- hemolyziny MeSH
- proteiny z Escherichia coli MeSH
E. faecalis (67%) and E. faecium (13.7%) were most frequently isolated among enterococci that contaminate cooled and frozen processed meat, follow-up heat-treated meat products and unheated fermented dry salami. Most isolates of both species were resistant to cephalothin (95 and 83 %) and clindamycin (77 and 67%, respectively). Furthermore, E. faecalis and E. faecium isolates were resistant to erythromycin (44 and 72%), tetracycline (34.5 and 17.4%), and streptomycin (13.3 and 4.3%, respectively). Only a few of the isolates were resistant to ampicillin, ampicillin-sulbactam, chloramphenicol, and vancomycin while all isolates were susceptible to gentamicin, penicillin, and teicoplanin. During the production of heat-treated meat products, numbers of resistant isolates increased in spite of the decreasing enterococcal contamination of the samples. An opposite situation was found in the production of fermented dry salami.
The resistance to antibiotics and the distribution of virulence factors in enterococci isolated from traditional Slovak sheep cheese bryndza was compared with strains from human infections. The occurrence of 4 enterococcal species was observed in 117 bryndza-cheese isolates. The majority of strains were identified as E. faecium (76 %) and E. faecalis (23 %). Several strains of E. durans and 1 strain of E. hirae were also present. More than 90 % of strains isolated from 109 clinical enterococci were E. faecalis, the rest belonged to E. faecium. The resistance to 6 antimicrobial substances (ampicillin, ciprofloxacin, higher concentration of gentamicin, nitrofurantoin, tetracycline and vancomycin) was tested in clinical and food enterococci. A higher level of resistance was found in clinical than in food strains and E. faecium had a higher resistance than E. faecalis; no resistance to vancomycin was detected. The occurrence of 3 virulence-associated genes, cylA (coding for hemolysin), gelE (coding for gelatinase) and esp (coding for surface protein) was monitored. Differences were found in the distribution of cylA gene between clinical and bryndza-cheese E. faecalis strains; in contrast to clinical strains (45 %), cylA gene was detected in 22 % of food isolates. The distribution of 2 other virulence factors, gelE and esp, was not significantly different in the two groups of E. faecalis strains. cylA and gelE genes were not detected in E. faecium but more than 70 % of clinical E. faecium were positive for esp, even thought none of the 79 E. faecium cheese isolates contained this gene.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence * MeSH
- bakteriální proteiny genetika metabolismus MeSH
- Enterococcus faecalis účinky léků patogenita MeSH
- Enterococcus faecium účinky léků patogenita MeSH
- Enterococcus klasifikace účinky léků izolace a purifikace patogenita MeSH
- faktory virulence genetika metabolismus MeSH
- grampozitivní bakteriální infekce mikrobiologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- ovce MeSH
- sýr mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriální proteiny MeSH
- faktory virulence MeSH
