Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.

• MeSH
• Bacterial Proteins analysis   MeSH
• Bacteriological Techniques methods   MeSH
• DNA, Bacterial chemistry   genetics   MeSH
• Electrophoresis, Polyacrylamide Gel MeSH
• Goats MeSH
• Limosilactobacillus reuteri classification   genetics   isolation & purification   physiology   MeSH
• DNA, Ribosomal Spacer chemistry   genetics   MeSH
• Sheep MeSH
• Polymerase Chain Reaction MeSH
• Reproducibility of Results MeSH
• Cluster Analysis MeSH
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
• Gastric Mucosa microbiology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Comparative Study MeSH
• Names of Substances
• Bacterial Proteins MeSH
• DNA, Bacterial MeSH
• DNA, Ribosomal Spacer MeSH

One-hundred sixty Escherichia coli isolates obtained from piglets with diarrhea from different parts of Slovakia were examined for the presence of genes coding for F4, F5, F6 and F41 fimbrial adhesins, and hemolytic activity. According to polymerase chain reaction tests 74 (46%) E. coli isolates were positive for primers that detected genes coding for fimbrial adhesins. Of these 74 isolates, 64 were positive for genes encoding for F4+, four for F5+, five for F6+, and one for both F41+ and F5+ adhesins.

• MeSH
• Adhesins, Escherichia coli genetics   MeSH
• Antigens, Bacterial genetics   MeSH
• Adhesins, Bacterial genetics   MeSH
• Bacterial Toxins biosynthesis   MeSH
• DNA, Bacterial analysis   genetics   MeSH
• Escherichia coli genetics   isolation & purification   MeSH
• Genotype MeSH
• Hemolysin Proteins biosynthesis   MeSH
• Escherichia coli Infections microbiology   veterinary   MeSH
• Swine Diseases microbiology   MeSH
• Polymerase Chain Reaction MeSH
• Swine microbiology   MeSH
• Escherichia coli Proteins genetics   MeSH
• Diarrhea microbiology   veterinary   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia MeSH
• Names of Substances
• Adhesins, Escherichia coli MeSH
• Antigens, Bacterial MeSH
• Adhesins, Bacterial MeSH
• Bacterial Toxins MeSH
• DNA, Bacterial MeSH
• F41 antigen, E coli MeSH Browser
• Hemolysin Proteins MeSH
• Escherichia coli Proteins MeSH

E. faecalis (67%) and E. faecium (13.7%) were most frequently isolated among enterococci that contaminate cooled and frozen processed meat, follow-up heat-treated meat products and unheated fermented dry salami. Most isolates of both species were resistant to cephalothin (95 and 83 %) and clindamycin (77 and 67%, respectively). Furthermore, E. faecalis and E. faecium isolates were resistant to erythromycin (44 and 72%), tetracycline (34.5 and 17.4%), and streptomycin (13.3 and 4.3%, respectively). Only a few of the isolates were resistant to ampicillin, ampicillin-sulbactam, chloramphenicol, and vancomycin while all isolates were susceptible to gentamicin, penicillin, and teicoplanin. During the production of heat-treated meat products, numbers of resistant isolates increased in spite of the decreasing enterococcal contamination of the samples. An opposite situation was found in the production of fermented dry salami.

• MeSH
• Drug Resistance, Bacterial MeSH
• Enterococcus faecalis drug effects   MeSH
• Enterococcus faecium drug effects   MeSH
• Meat Products microbiology   MeSH
• Meat microbiology   MeSH
• Cattle MeSH
• Animals MeSH
• Check Tag
• Cattle MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

The resistance to antibiotics and the distribution of virulence factors in enterococci isolated from traditional Slovak sheep cheese bryndza was compared with strains from human infections. The occurrence of 4 enterococcal species was observed in 117 bryndza-cheese isolates. The majority of strains were identified as E. faecium (76 %) and E. faecalis (23 %). Several strains of E. durans and 1 strain of E. hirae were also present. More than 90 % of strains isolated from 109 clinical enterococci were E. faecalis, the rest belonged to E. faecium. The resistance to 6 antimicrobial substances (ampicillin, ciprofloxacin, higher concentration of gentamicin, nitrofurantoin, tetracycline and vancomycin) was tested in clinical and food enterococci. A higher level of resistance was found in clinical than in food strains and E. faecium had a higher resistance than E. faecalis; no resistance to vancomycin was detected. The occurrence of 3 virulence-associated genes, cylA (coding for hemolysin), gelE (coding for gelatinase) and esp (coding for surface protein) was monitored. Differences were found in the distribution of cylA gene between clinical and bryndza-cheese E. faecalis strains; in contrast to clinical strains (45 %), cylA gene was detected in 22 % of food isolates. The distribution of 2 other virulence factors, gelE and esp, was not significantly different in the two groups of E. faecalis strains. cylA and gelE genes were not detected in E. faecium but more than 70 % of clinical E. faecium were positive for esp, even thought none of the 79 E. faecium cheese isolates contained this gene.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Drug Resistance, Bacterial * MeSH
• Bacterial Proteins genetics   metabolism   MeSH
• Enterococcus faecalis drug effects   pathogenicity   MeSH
• Enterococcus faecium drug effects   pathogenicity   MeSH
• Enterococcus classification   drug effects   isolation & purification   pathogenicity   MeSH
• Virulence Factors genetics   metabolism   MeSH
• Gram-Positive Bacterial Infections microbiology   MeSH
• Humans MeSH
• Microbial Sensitivity Tests MeSH
• Sheep MeSH
• Cheese microbiology   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Bacterial Proteins MeSH
• Virulence Factors MeSH