The influence of Saccharomyces cerevisiae var. vini viable cells on the Staphylococcus aureus-suffering mice was ascertained: (1) effect of S. aureus living cells on antibacterial and antitoxic antibodies, (2) effect of S. aureus on the number of neurons and macroglial cells in different areas of mouse hippocampus, (3) effect of S. cerevisiae var. vini viable cells on the above-mentioned changes of immune and nervous system. Treatment with S. cerevisiae var. vini provokes immune stimulation and change of the total number of macroglial elements in the CA1 field of hippocampus.

• MeSH
• hipokampus patologie   MeSH
• histocytochemie MeSH
• mikroglie patologie   MeSH
• modely nemocí na zvířatech MeSH
• mozek patologie   MeSH
• myši MeSH
• neurony patologie   MeSH
• probiotika aplikace a dávkování   MeSH
• protilátky bakteriální krev   MeSH
• Saccharomyces cerevisiae růst a vývoj   MeSH
• stafylokokové infekce imunologie   mikrobiologie   patologie   terapie   MeSH
• Staphylococcus aureus imunologie   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protilátky bakteriální MeSH

A simple assay by polymerase chain reaction was used for the of detection of Borrelia burgdorferi, causative agent of Lyme borreliosis (LB). It involves no DNA purification and is based on the amplification of a specific region of ospA gene of B. burgdorferi, followed by direct detection of the PCR product with SYBR Green I by agarose gel electrophoresis. The method was used to analyze samples from patients with LB diagnosis, with presumable infection with the LB spirochete, those with unclear clinical symptoms and after the course of an antibiotic treatment. Spirochetal DNA was detected by PCR even in contaminated samples in which B. burgdorferi was overgrown by fungi and other bacteria. Spirochetal DNA was detected and borrelia species was identified in cerebrospinal fluid of two patients hospitalized with the diagnosis "fever of unknown origin". Western blot and ELISA were negative in both cases. Total analysis of 94 samples from the hospital in Ceské Budejovice (South Bohemia, Czechia) showed infection with B. burgdorferi sensu stricto in 11% and B. garinii in 15% of cases. The highest prevalence was found for B. afzelii (43%). Co-infection was confirmed in 24 % of the analyzed symplex; 7% of samples that were B. burgdorferi sensu lato positive gave no results in DNA amplification with B. burgdorferi sensu stricto-, B. garinii- and B. afzelii-specific primers. The proposed reliable, rapid, unexpensive and specific technique could form the basis of laboratory tests for routine detection and identification of Lyme-disease spirochete in different samples.

• MeSH
• antigeny povrchové genetika   MeSH
• bakteriální vakcíny MeSH
• benzothiazoly MeSH
• Borrelia burgdorferi komplex klasifikace   genetika   imunologie   izolace a purifikace   MeSH
• chinoliny MeSH
• diaminy MeSH
• DNA bakterií analýza   izolace a purifikace   MeSH
• elektroforéza v agarovém gelu MeSH
• ELISA MeSH
• genotyp MeSH
• imunoglobulin G krev   MeSH
• imunoglobulin M krev   MeSH
• lidé MeSH
• lipoproteiny genetika   MeSH
• lymeská nemoc diagnóza   MeSH
• mozkomíšní mok mikrobiologie   MeSH
• organické látky metabolismus   MeSH
• polymerázová řetězová reakce metody   MeSH
• proteiny vnější bakteriální membrány genetika   MeSH
• protilátky bakteriální krev   MeSH
• senzitivita a specificita MeSH
• sérum mikrobiologie   MeSH
• techniky typizace bakterií MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny povrchové MeSH
• bakteriální vakcíny MeSH
• benzothiazoly MeSH
• chinoliny MeSH
• diaminy MeSH
• DNA bakterií MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• lipoproteiny MeSH
• organické látky MeSH
• OspA protein MeSH Prohlížeč
• proteiny vnější bakteriální membrány MeSH
• protilátky bakteriální MeSH
• SYBR Green I MeSH Prohlížeč

Borrelial glycoconjugates were localized by labeled lectins on ultrathin cryosections and on surfaces of intact negatively stained bacteria. Protein-saccharide complexes in these glycoconjugates were partially characterized by means of enzyme deglycosylation and mild alkali pretreatment of cryosections. The results of labeling were examined by transmission electron microscopy. Statistically evaluated results (relative labeling index, chi2 test) of gold labeling indicated that surfaces of Borrelia burgdorferi strain B31 and external (outer) membrane vesicles (MVs) were covered with glycoconjugates containing O-glycosidically linked N-acetyl-D-galactosamine (GalNAc) and N-glycosidically linked N-acetyl-D-glucosamine (GlcNAc). The presence of N-linked GalNAc, sialic acid, mannose and fucose on the surfaces of outer membranes and MVs was probably due to an adherence of BSK-H medium components, especially rabbit serum, to Borrelia surfaces.

• MeSH
• Borrelia burgdorferi metabolismus   patogenita   ultrastruktura   MeSH
• buněčná membrána metabolismus   ultrastruktura   MeSH
• elektronová kryomikroskopie MeSH
• glykokonjugáty chemie   metabolismus   MeSH
• králíci MeSH
• lektiny metabolismus   MeSH
• lidé MeSH
• lymeská nemoc mikrobiologie   MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• glykokonjugáty MeSH
• lektiny MeSH

The impact of Ixodes ricinus salivary gland extract (SGE) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen, B. afzelii, was determined using flow cytometry. SGE injected together with spirochetes reduced the numbers of leukocytes and gammadelta-T lymphocytes in infected epidermis at early time-points post infection. In draining lymph nodes, the anti-inflammatory effect of SGE was manifested by the decrease of total cell count compared with that in mice treated with inactivated SGE. Changes in subpopulations of immunocompetent cells apparently reflected the effect of SGE on the proliferation of spirochetes in the host. The significance of tick saliva anti-inflammatory effect for saliva activated transmission of B. afzelii is shown.

• MeSH
• Borrelia burgdorferi komplex růst a vývoj   MeSH
• klíšťata imunologie   MeSH
• kůže imunologie   patologie   MeSH
• lymeská nemoc imunologie   patologie   přenos   MeSH
• lymfatické uzliny imunologie   patologie   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• počet lymfocytů MeSH
• podskupiny lymfocytů MeSH
• slinné žlázy imunologie   MeSH
• tkáňové extrakty imunologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• tkáňové extrakty MeSH