Nejvíce citovaný článek - PubMed ID 20179964
Heterotrophic carbon gain by the root hemiparasites, Rhinanthus minor and Euphrasia rostkoviana (Orobanchaceae)
Root-hemiparasitic plants of the genus Rhinanthus acquire resources through a water-wasting physiological strategy based on high transpiration rate mediated by the accumulation of osmotically active compounds and constantly open stomata. Interestingly, they were also documented to withstand moderate water stress which agrees with their common occurrence in rather dry habitats. Here, we focused on the water-stress physiology of Rhinanthus alectorolophus by examining gas exchange, water relations, stomatal density, and biomass production and its stable isotope composition in adult plants grown on wheat under contrasting (optimal and drought-inducing) water treatments. We also tested the effect of water stress on the survival of Rhinanthus seedlings, which were watered either once (after wheat sowing), twice (after wheat sowing and the hemiparasite planting) or continuously (twice and every sixth day after that). Water shortage significantly reduced seedling survival as well as the biomass production and gas exchange of adult hemiparasites. In spite of that drought-stressed and even wilted plants from both treatments still considerably photosynthesized and transpired. Strikingly, low-irrigated plants exhibited significantly elevated photosynthetic rate compared with high-irrigated plants of the same water status. This might relate to biochemical adjustments of these plants enhancing the resource uptake from the host. Moreover, low-irrigated plants did not acclimatize to water stress by lowering their osmotic potential, perhaps due to the capability to tolerate drought without such an adjustment, as their osmotic potential at full turgor was already low. Contrary to results of previous studies, hemiparasites seem to close their stomata in response to severe drought stress and this happens probably passively after turgor is lost in guard cells. The physiological traits of hemiparasites, namely the low osmotic potential associated with their parasitic lifestyle and the ability to withstand drought and recover from the wilting likely enable them to grow in dry habitats. However, the absence of osmotic adjustment of adults and sensitivity of seedlings to severe drought stress demonstrated here may result in a substantial decline of the hemiparasitic species with ongoing climate change.
- MeSH
- biomasa MeSH
- dehydratace MeSH
- fotosyntéza MeSH
- fyziologický stres fyziologie MeSH
- kořeny rostlin MeSH
- listy rostlin anatomie a histologie fyziologie MeSH
- Orobanchaceae anatomie a histologie fyziologie MeSH
- průduchy rostlin anatomie a histologie fyziologie MeSH
- semenáček anatomie a histologie fyziologie MeSH
- transpirace rostlin MeSH
- voda * metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- voda * MeSH
BACKGROUND AND AIMS: Root hemiparasites from the rhinanthoid clade of Orobanchaceae possess metabolically active glandular trichomes that have been suggested to function as hydathode trichomes actively secreting water, a process that may facilitate resource acquisition from the host plant's root xylem. However, no direct evidence relating the trichomes to water secretion exists, and carbon budgets associated with this energy-demanding process have not been determined. METHODS: Macro- and microscopic observations of the leaves of hemiparasitic Rhinanthus alectorolophus were conducted and night-time gas exchange was measured. Correlations were examined among the intensity of guttation, respiration and transpiration, and analysis of these correlations allowed the carbon budget of the trichome activity to be quantified. We examined the intensity of guttation, respiration and transpiration, correlations among which indicate active water secretion. KEY RESULTS: Guttation was observed on the leaves of 50 % of the young, non-flowering plants that were examined, and microscopic observations revealed water secretion from the glandular trichomes present on the abaxial leaf side. Night-time rates of respiration and transpiration and the presence of guttation drops were positively correlated, which is a clear indicator of hydathode trichome activity. Subsequent physiological measurements on older, flowering plants indicated neither intense guttation nor the presence of correlations, which suggests that the peak activity of hydathodes is in the juvenile stage. CONCLUSIONS: This study provides the first unequivocal evidence for the physiological role of the hydathode trichomes in active water secretion in the rhinanthoid Orobanchaceae. Depending on the concentration of organic elements calculated to be in the host xylem sap, the direct effect of water secretion on carbon balance ranges from close to neutral to positive. However, it is likely to be positive in the xylem-only feeding holoparasites of the genus Lathraea, which is closely related to Rhinanthus. Thus, water secretion by the hydathodes might be viewed as a physiological pre-adaptation in the evolution of holoparasitism in the rhinanthoid lineage of Orobanchaceae.
- Klíčová slova
- Ecophysiology, Lathraea, Rhinanthus alectorolophus, Triticum aestivum, holoparasite, hydathode trichome, orobanche, parasitic plant, respiration, rhinanthoid Orobanchaceae, root hemiparasite, transpiration, water regime, water secretion, xylem,
- MeSH
- biologická evoluce * MeSH
- buněčné dýchání MeSH
- interakce hostitele a parazita fyziologie MeSH
- kořeny rostlin parazitologie MeSH
- lineární modely MeSH
- listy rostlin metabolismus MeSH
- Orobanchaceae fyziologie MeSH
- plyny metabolismus MeSH
- transpirace rostlin MeSH
- trichomy fyziologie MeSH
- voda metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- plyny MeSH
- voda MeSH
In their recent study, Borowicz and Armstrong (Oecologia 169:783-792, 2012) investigated effects of nutrient availability and competition for light on a perennial root hemiparasite Pedicularis canadensis. Their study showed a reduction of community productivity as a result of hemiparasite infection independently of a clear positive effect of increased nutrients. In contrast, there was a minimal effect of increased competition for light on growth of the parasite. Here, we summarize the available data on the influence of nutrient availability (closely related to productivity) on temperate grassland root hemiparasites thus expanding the discussion presented by Borowicz and Armstrong (Oecologia 169:783-792, 2012). Most studies show that root hemiparasites are highly sensitive to elevated competition for light in productive environments, which is manifested as an increase in mortality coupled to a decrease in population density. Such responses reflect increased mortality of hemiparasite seedlings that are physiologically inefficient in terms of photosynthesis and nutrient acquisition owing to a limited root network and consequently, are highly sensitive to competition for light. However, the susceptibility of hemiparasites to competition for light tends to decrease for individuals that survive the critical seedling stage. Moreover, survivors benefit from elevated nutrient availability, resulting in increased growth and fecundity. Elevated productivity can thus have opposing effects on the survival and growth of hemiparasites depending on life stage. We conclude that the findings by Borowicz and Armstrong (Oecologia 169:783-792, 2012) are not in conflict with this general view that root hemiparasite population ecology is strongly influenced by competition for light in highly productive environments.
Hemiparasitic plants display a unique strategy of resource acquisition combining parasitism of other species and own photosynthetic activity. Despite the active photoassimilation and green habit, they acquire substantial amount of carbon from their hosts. The organic carbon transfer has a crucial influence on the nature of the interaction between hemiparasites and their hosts which can oscillate between parasitism and competition for light. In this minireview, we summarize methodical approaches and results of various studies dealing with carbon budget of hemiparasites and the ecological implications of carbon heterotrophy in hemiparasites.
