A nonpathogenic bacterium of external environment possessing remarkable immunomodulatory activity, Bacillus firmus (BF) inactivated with formaldehyde, was given intragastrically to two genetically different mouse strains BALB/c (H-2d) and B10.BR/SnPh (B10.BR, H-2k) reared in conventional (CV) and B10.BR strain also in germ-free (GF) conditions. Repeated intragastric administration of BF (500 micrograms every other day over two weeks, starting at the age of 3 months) significantly enhanced intestinal IgA levels in CV BALB/c mice but did not affect intestinal IgA in CV B10.BR mice. In GF B10.BR mice, IgG levels in sera and intestinal washings increased after BF administration compared to CV B10.BR mice. In CV BALB/c mice, specific activity of enterocyte brush-border enzymes (lactase, gamma-glutamyltransferase, alkaline phosphatase) decreased after BF treatment; sucrase (sucrose alpha-glucosidase) activity was not affected. On the other hand, in B10.BR mice, specific activity of gamma-glutamyltransferase and dipeptidyl peptidase IV were higher after administration of BF in both CV and GF groups relative to untreated controls. The activities of lactase and glucoamylase (glucan 1,4-alpha-glucosidase) were significantly stimulated only in the group of GF B10.BR mice treated with formolized BF. The stimulation of immunoglobulin production after BF treatment was accompanied by changes in the levels of enterocyte brush-border enzymes; this responsiveness to BF treatment was genetically regulated.

• MeSH
• alkalická fosfatasa metabolismus   MeSH
• Bacillus imunologie   MeSH
• beta-galaktosidasa metabolismus   MeSH
• dipeptidylpeptidasa 4 metabolismus   MeSH
• enterocyty enzymologie   mikrobiologie   MeSH
• genetická predispozice k nemoci MeSH
• glukoamylasa metabolismus   MeSH
• gnotobiologické modely MeSH
• imunoglobulin A biosyntéza   krev   MeSH
• imunohistochemie MeSH
• laktasa MeSH
• mikroklky enzymologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• sacharasa metabolismus   MeSH
• střeva enzymologie   imunologie   mikrobiologie   MeSH
• střevní sliznice enzymologie   imunologie   mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alkalická fosfatasa MeSH
• beta-galaktosidasa MeSH
• dipeptidylpeptidasa 4 MeSH
• glukoamylasa MeSH
• imunoglobulin A MeSH
• laktasa MeSH
• sacharasa MeSH

The effect of controlled antigenic stimulation in immunologically virgin organisms, i.e. pig fetuses treated with NDCM (Nocardia delipidated cell mitogen) and germ-free (GF) piglets associated with a non-pathogenic E. coli 086, on peripheral blood lymphocyte subsets defined by the expression of CD5 and CD8 was studied by double color flow cytometry. Stimulation of both fetuses and GF piglets increased the frequency of CD8low+ lymphocytes. A prominent subset of CD5-CD8low+NK cells was present in GF and E. coli associated piglets and their frequency was slightly higher in E. coli associated animals. The most pronounced difference between stimulated and non-stimulated animals was in a relative proportion of an ill-defined lymphocyte subset with an unusual CD5low+CD8low+ expression. Both NDCM injection into fetal blood circulation and association of GF piglets with E. coli resulted in a marked increase of frequency of CD5low+CD8low+ lymphocytes in peripheral blood.

• MeSH
• adjuvancia imunologická * MeSH
• aktivace lymfocytů * MeSH
• antigeny CD5 analýza   MeSH
• antigeny CD8 analýza   MeSH
• Escherichia coli imunologie   MeSH
• gnotobiologické modely MeSH
• imunizace * MeSH
• imunofenotypizace MeSH
• plod MeSH
• podskupiny lymfocytů imunologie   MeSH
• prasata MeSH
• průtoková cytometrie MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adjuvancia imunologická * MeSH
• antigeny CD5 MeSH
• antigeny CD8 MeSH

The germ-free pig model is shown to be useful for studying salmonellosis. The immune status of germ-free and infected gnotobiotic piglets is described. The regulatory role of cytokine is discussed and compared with our experimental findings.

• MeSH
• B-lymfocyty mikrobiologie   MeSH
• imunoglobulin A krev   MeSH
• imunoglobulin G krev   MeSH
• imunoglobulin M krev   MeSH
• interferon gama krev   MeSH
• modely nemocí na zvířatech * MeSH
• organismy bez specifických patogenů MeSH
• prasata * MeSH
• protilátky bakteriální krev   MeSH
• Salmonella typhimurium imunologie   MeSH
• salmonelová infekce u zvířat imunologie   mikrobiologie   MeSH
• TNF-alfa metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• imunoglobulin A MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• interferon gama MeSH
• protilátky bakteriální MeSH
• TNF-alfa MeSH