Pleurotus ostreatus is one of the most cultivated mushrooms in the Czech Republic. The production of 1 kg of mushrooms generates about 5 kg of spent mushroom substrate. A gentle and fast method for using this substrate is vermicomposting. Vermicomposting of spent mushroom substrate using Eisenia andrei was conducted for seven months. For control purposes, a treatment without earthworms was also prepared. The vermicomposting process used vertical continuous feeding vermicomposters. The agrochemical and biological parameters were analysed. Values of electrical conductivity were very high in both vermicomposters (higher than 2000 μS/cm). During the vermicomposting process the C/N ratio decreased. The number and biomass of earthworms decreased with the age of the layers. The values of total P, K and Mg were higher in the vermicomposter without earthworms. There were also lower microbial phospholipid fatty acids content - than in the vermicomposter with earthworms. However, the fungal phospholipid fatty acids content were two times higher in the vermicomposter without earthworms. The highest hydrolytic enzyme activity was found in lipase, acid phosphatase and β-D-glucosidase. Most hydrolytic enzymes were more active in the vermicomposter without earthworms, with the exception of arylsulphatase. Mn-peroxidase activity was higher in the vermicomposter without earthworms and laccase activity was below the detection limit.

• Klíčová slova
• Continuous feeding, Enzymatic activity, PLFA analysis, Pleurotus ostreatus, Spent mushroom substrate, Vermicomposting,
• MeSH
• Agaricales * MeSH
• agrochemikálie MeSH
• Oligochaeta * MeSH
• Pleurotus * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• agrochemikálie MeSH

The influence of environmental parameters on mycelial linear growth of Pleurotus ostreatus, P. eryngii, P. pulmonarius, Agrocybe aegerita, Lentinula edodes, Volvariella volvacea and Auricularia auricula-judae was determined in two different nutrient media in a wide range of temperature, forming the basis for the assessment of their temperature optima. V. volvacea grew faster at 35 degrees C, P. eryngii at 25 degrees C, P. ostreatus and P. pulmonarius at 30 degrees C, A. aegerita at 25 or 30 degrees C and A. auricula-judae at 20 or 25 degrees C depending on the nutrient medium used and L. edodes at 20 or 30 degrees C depending on the strain examined. The mycelium extension rates were evaluated on seven mushroom cultivation substrates: wheat straw, cotton gin-trash, peanut shells, poplar sawdust, oak sawdust, corn cobs and olive press-cake. The mycelium extension rates (linear growth and colonization rates) were determined by the 'race-tube' technique, and were found to be the highest on cotton gin-trash, peanut shells and poplar sawdust for Pleurotus spp. and A. aegerita. Wheat straw, peanut shells and particularly cotton gin-trash supported fast growth of V. volvacea, whereas wheat straw was the most suitable substrate for L. edodes and A. auricula-judae. Supplemented oak sawdust and olive press-cake were poor substrates for most species examined, while almost all strains performed adequately on corn cobs.

• MeSH
• Agaricales růst a vývoj   metabolismus   MeSH
• celulosa metabolismus   MeSH
• lignin metabolismus   MeSH
• mycelium růst a vývoj   metabolismus   MeSH
• potravinářský průmysl metody   MeSH
• teplota MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• celulosa MeSH
• lignin MeSH
• lignocellulose MeSH Prohlížeč

King oyster mushroom Pleurotus eryngii is cultivated worldwide for culinary and to improve human health. However, the potential of some Mediterranean representatives of this species is still not evaluated. This work focuses on the study of polysaccharides from fruiting bodies of two Tunisian strains, P. eryngii var. elaeoselini and P. eryngii var. ferulae, and, for comparison, one deposited P. eryngii originated from Korea. Polysaccharides were successively extracted with hot water using microwave heating and 1 mol L-1 aqueous sodium hydroxide. The crude hot water extracts were purified by treating them with proteolytic enzymes, and the alkaline extracts were purified by re-dissolving with dimethyl sulphoxide. In both cases, a decrease or removal of proteins was detected. Glucans predominated in all these products; the insoluble parts also contained chitin. The purified hot water extracts contained glycogen, β-d-glucans and mannogalactan. Branching (1 → 3)(1 → 6)-β-d-glucan was the major polysaccharide in the alkali-soluble fractions, while (1 → 3)-α-d-glucan was only a minor component. The Tunisian strains demonstrated a higher proportion of water-soluble polysaccharides, compared to the alkaline soluble ones, and more β-d-glucan in the insoluble chitin-glucan complexes. Fruiting body proteins of these strains are more available for solubilisation and enzymatic or alkaline degradation and, thus, may have higher nutritional value than those of the reference strain. As a source of proteins or polysaccharides, the Tunisian endemic P. eryngii strains of this study are promising for the domestication and cultivation of fruiting bodies for gastronomic purposes in the North African region.

• Klíčová slova
• Extraction, King oyster mushroom, Polysaccharides, Structure,
• MeSH
• druhová specificita MeSH
• fungální polysacharidy chemie   MeSH
• Pleurotus * chemie   MeSH
• plodnice hub * chemie   MeSH
• polysacharidy chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fungální polysacharidy MeSH
• polysacharidy MeSH

Cadmium (Cd) and its mobility and toxicity in the food chain receives considerable public and scientific attention. Levels in soil have been increasing, partly caused by mineral fertiliser use. In many areas in the EU, intake of Cd is not far from the maximum tolerable level. Now, urban and more circular approaches to food production are being promoted, in which waste materials substitute growing media and fertilizers. Can this make the situation worse, or is it, on the contrary, possible to extract Cd from the cycle using practical microbial methods? Cd concentration, availability and accumulation in materials and crops in a closed greenhouse system was investigated first. Secondly, we tested if fungal remediation was able to remove a significant amount of Cd. A sequential extraction analysis showed how anaerobic digestion and composting changed chemical Cd availability. Cd levels in the system were comparable to agricultural soils. The vegetable crops did not accumulate Cd. However, the culture of the edible mushroom Agaricus subrufescens in the greenhouse had a strong effect on Cd mobility: It extracted 80% of the Cd from the growing medium into the mushrooms, mostly at the start of fruiting. These early Cd enriched mushrooms can be treated as special waste, while the later harvest complied with EU Cd limits for foodstuffs and can potentially be consumed. Thus, growing this mushroom species in an integrated waste-to-crop system can be a cost-efficient way to remediate Cd from the food chain.

• Klíčová slova
• Agaricus subrufescens, Bioconcentration, Bioremediation, Digestate, Food waste, Mushroom,
• MeSH
• Agaricus * MeSH
• kadmium MeSH
• průmyslová hnojiva MeSH
• půda MeSH
• zemědělství MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kadmium MeSH
• průmyslová hnojiva MeSH
• půda MeSH

Oyster mushrooms are an interesting source of biologically active glucans and other polysaccharides. This work is devoted to the isolation and structural characterization of polysaccharides from basidiocarps of the cultivated oyster mushroom, Pleurotus ostreatus. Five polysaccharidic fractions were obtained by subsequent extraction with cold water, hot water and two subsequent extractions with 1 m sodium hydroxide. Branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan predominated in cold- and hot-water-soluble fractions, respectively. Alternatively, these polysaccharides were obtained by only hot water extraction and subsequent two-stage chromatographic separation. The alkali-soluble parts originating from the first alkali extraction were then fractionated by dissolution in dimethyl sulfoxide (DMSO). The polysaccharide insoluble in DMSO was identified as linear (1→3)-α-d-glucan, while branched (1→3)(1→6)-β-d-glucans were found to be soluble in DMSO. The second alkaline extract contained the mentioned branched β-d-glucan together with some proteins. Finally, the alkali insoluble part was a cell wall complex of chitin and β-d-glucans.

• Klíčová slova
• basidiocarps, fractionation, glucans, mannogalactan, oyster mushrooms, polysaccharides,
• MeSH
• chemická frakcionace MeSH
• chromatografie MeSH
• fungální polysacharidy chemie   izolace a purifikace   MeSH
• fytonutrienty chemie   izolace a purifikace   MeSH
• glukany chemie   MeSH
• molekulární struktura MeSH
• monosacharidy chemie   MeSH
• Pleurotus chemie   MeSH
• plodnice hub chemie   MeSH
• spektrální analýza MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fungální polysacharidy MeSH
• fytonutrienty MeSH
• glukany MeSH
• monosacharidy MeSH

Psilocybe cubensis, a widely recognized psychoactive mushroom species, has played a significant role in both historical and modern therapeutic practices. This review explores the complex interplay between genetic diversity, strain variability and environmental factors that shape the biosynthesis of key psychoactive compounds, including psilocybin and psilocin. With many strains exhibiting substantial variability in their phenotypic characteristics and biochemical content, understanding and documenting this diversity is crucial for optimizing therapeutic applications. The review also highlights advances in cultivation techniques, such as submerged fermentation of the mycelium, and innovative analytical methodologies that have improved the precision of compound quantification and extraction. Although there is limited scientific information on P. cubensis due to nearly four decades of regulatory restrictions on psychedelic research, recent developments in genetic and biochemical studies are beginning to provide valuable insights into its therapeutic potential. Furthermore, this review emphasizes key knowledge gaps and offers insights into future research directions to advance the cultivation, scientific documentation of strain diversity, regulatory considerations and therapeutic use of P. cubensis.

• Klíčová slova
• Psilocybe, fungi, genetics, mushroom, mycelium, psilocin, psilocybin, psychoactive,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

World mushroom production in 2018 was approximately 8.99 million tonnes. The most commonly cultivated species in the Czech Republic are Agaricus spp., which are sold fresh or canned. In 2017, 2018 mushroom production in the Czech Republic was approximately 540 tonnes. Vermicomposting is an easy and less ecologically harmful way to process the spent mushroom substrate. Earthworms, which are referred to as the engine of the process of vermicomposting, and microorganisms, help convert organic waste into fertilizer. This study is concerned with laboratory vermicomposting in a system of continuous feeding of earthworms Eisenia andrei. It compares the differences between variants with and without earthworms. The dry matter percentage was approximately 20% or more in both variants. The variant with earthworms showed a significant decrease in electrical conductivity. The C/N ratio was very low in both variants. The highest total P was recorded in the variant with earthworms, but the highest values of K and Mg were found in the control. Both variants recorded higher content of bacteria than fungi. All values of microorganism contents were higher in the vermicomposter without earthworms, but the bacterial/fungal ratio was higher in the variant with earthworms. The highest content in both variants shows the bacteria especially G-bacteria, on the other side, the lowest content shows the actinobacteria. The highest activity of β-D-glucosidase and acid phosphatase was measured in the vermicomposter with earthworms, but the activity of other enzymes was higher in the control. In both vermicomposters laccase activity was below the detection limit. The method of classical vermicomposting can be used for processing the spent mushroom substrate. However, in terms of higher content of total and available nutrients, there seems to be a better method of processing the substrate without earthworms.

• Klíčová slova
• Agaricus subrufescens, Continuous feeding, Enzymatic activity, PLFA analysis, Spent mushroom substrate, Vermicomposting,
• MeSH
• Agaricus * MeSH
• Bacteria MeSH
• houby MeSH
• Oligochaeta * MeSH
• půda MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• půda MeSH

Mushrooms fortified with iron (Fe) can offer a promising alternative to counter the worldwide deficiency problem. However, the factors that may influence the efficiency of fortification have not yet been fully investigated. The aim of this study was to compare the effects of three Fe forms (FeCl3 6H2O, FeSO4 7H2O, or FeHBED) in three concentrations (5, 10, or 50 mM) for three mushroom species (Pleurotus eryngii, P. ostreatus, or Pholiota nameko) on their chemical composition, phenolic compounds, and organic acid production. The most effective metal accumulation of all the investigated species was for the 50 mM addition. FeCl3 6H2O was the most favorable additive for P. eryngii and P. nameko (up to 145 and 185% Fe more than in the control, respectively) and FeHBED for P. ostreatus (up to 108% Fe more than in control). Additionally, P. nameko showed the highest Fe accumulation among studied species (89.2 ± 7.51 mg kg-1 DW). The creation of phenolic acids was generally inhibited by Fe salt supplementation. However, an increasing effect on phenolic acid concentration was observed for P. ostreatus cultivated at 5 mM FeCl3 6H2O and for P. eryngii cultivated at 5 mM FeCl3 6H2O and 5 mM FeSO4 7H2O. In the case of organic acids, a similar situation was observed. For P. ostreatus, FeSO4 7H2O and FeHBED salts increased the formation of the determined organic acids in fruiting bodies. P. eryngii and P. nameko were characterized by a much lower content of organic acids in the systems supplemented with Fe. Based on the obtained results, we recommend starting fortification by preliminarily indicating which form of the element is preferred for the species of interest for supplementation. It also seems that using an additive concentration of 50 mM or higher is most effective.

• Klíčová slova
• Pholiota nameko, Pleurotus eryngii, Pleurotus ostreatus, deficiency, functional food, malnutrition, organic acids, phenolic acids, supplementation,
• MeSH
• Agaricus * MeSH
• biofortifikace MeSH
• Pleurotus * chemie   MeSH
• soli MeSH
• železo MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• soli MeSH
• železo MeSH

Pleurotus pulmonarius was cultivated on a corncob-based substrate for producing of mushrooms and for assessing the transformation of the lignocellulosics during the development of fungal biomass. Associated events, such as the release of relevant enzymes and the H2O2 generation, were also monitored. The peaks of laccase and catalase activities occurred at the 5th day and that of Mn peroxidase at the 30th day, simultaneously with a high activity of superoxide dismutase. Increase in the endocellulase and xylanase activities was observed after 10 days, with maximal activities achieved during the 20-30-day period. Maximal values of H2O2 were found after 10 days of cultivation. Electron microscopy and Fourier transform infrared (FTIR) spectroscopy showed strong alterations in the lignocellulosic fibers. The uncultivated and the cultivated substrates at different times were hydrolyzed with commercial cellulase and β-glucosidase. The highest values of reducing sugars (110.5 ± 5.6 μmol/mL), being 65 % glucose, were obtained using the 20-day cultivated substrate. After the fruiting stage (first flush), enzymatic hydrolysis of the spent mushroom substrate (SMS) yielded 53.0 ± 2.8 and 77.5 ± 4.0 μmol/mL of glucose and total reducing sugars, respectively. Although the release of reducing sugars of the P. pulmonarius SMS was lower than that obtained after 20 days of cultivation, it was still 50 % higher than that obtained using the uncultured substrate. This observation, combined with the fact that SMS constitutes a residue generated as a by-product of the depletion of an agro-industrial residue, allows to conclude that this material offers an interesting economic perspective for the obtainment of cellulosic ethanol.

• MeSH
• elektronová mikroskopie MeSH
• enzymy analýza   MeSH
• kukuřice setá chemie   mikrobiologie   ultrastruktura   MeSH
• lignin analýza   MeSH
• peroxid vodíku analýza   MeSH
• Pleurotus růst a vývoj   metabolismus   MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• enzymy MeSH
• lignin MeSH
• lignocellulose MeSH Prohlížeč
• peroxid vodíku MeSH

Agaritine (N-(gamma-L(+)-glutamyl)-4-hydroxymethyl-phenylhydrazine) was identified and quantified by high-pressure liquid chromatography and used as a marker for the occurrence of phenylhydrazine derivatives in the cultivated Agaricus bitorquis and A. garicus hortensis mushrooms. Although relatively high levels of agaritine (around 700 mg kg(-1)) could be found in freshly harvested A. bitorquis from early flushes, samples from supermarkets contained less agaritine. The content of 28 samples varied between 165 and 457 mg kg(-1), on average being 272 +/- 69 mg kg(-1). The highest amounts of agaritine were found in the skin of the cap and in the gills, the lowest being in the stem. There was no significant difference in agaritine content of the two mushroom species in our study. Pronounced reduction in agaritine content was observed during storage of mushrooms in the refrigerator or freezer, as well as during drying of the mushrooms. The degree of reduction was dependent on the length and condition of storage and was usually in the region 20-75%. No reduction in agaritine content was observed during freeze-drying. Depending on the cooking procedure, household processing of cultivated Agaricus mushrooms reduced the agaritine content to various degrees. Boiling extracted around 50% of the agaritine content into the cooking broth within 5min and degraded 20-25% of the original agaritine content of the mushrooms. Prolonged boiling, as when preparing a sauce, reduced the content in the solid mushroom further (around 10% left after 2h). Dry baking of the cultivated mushroom, a process similar to pizza baking, reduced the agaritine content by approximately 25%, whereas frying in oil or butter or deep frying resulted in a more marked reduction (35-70%). Microwave processing of the cultivated mushrooms reduced the agaritine content to one-third of the original level. Thus, the exposure to agaritine was substantially less when consuming processed Agaricus mushrooms as compared with consuming the raw mushrooms. However, it is not yet known to what extent agaritine and other phenylhydrazine derivatives occurring in the cultivated mushroom are degraded into other biologically active compounds during the cooking procedure.

• MeSH
• Agaricus chemie   MeSH
• časové faktory MeSH
• fenylhydraziny škodlivé účinky   analýza   MeSH
• kulturní různorodost MeSH
• lidé MeSH
• manipulace s potravinami metody   MeSH
• spotřebitelská bezpečnost produktů normy   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• agaritine MeSH Prohlížeč
• fenylhydraziny MeSH