hierarchically structured
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The pseudo 3D hierarchical structure mimicking in vivo microenvironment was prepared by phase separation on tissue culture plastic. For surface treatment, time-sequenced dosing of the solvent mixture with various concentrations of polymer component was used. The experiments showed that hierarchically structured surfaces with macro, meso and micro pores can be prepared with multi-step phase separation processes. Changes in polystyrene surface topography were characterized by atomic force microscopy, scanning electron microscopy and contact profilometry. The cell proliferation and changes in cell morphology were tested on the prepared structured surfaces. Four types of cell lines were used for the determination of impact of the 3D architecture on the cell behavior, namely the mouse embryonic fibroblast, human lung carcinoma, primary human keratinocyte and mouse embryonic stem cells. The increase of proliferation of embryonic stem cells and mouse fibroblasts was the most remarkable. Moreover, the embryonic stem cells express different morphology when cultured on the structured surface. The acquired findings expand the current state of knowledge in the field of cell behavior on structured surfaces and bring new technological procedures leading to their preparation without the use of problematic temporary templates or additives.
- Klíčová slova
- foams, hierarchically structured, line-specific response, phase inversion, phase separations, stem cells, surfaces,
- MeSH
- fibroblasty * MeSH
- mikroskopie atomárních sil MeSH
- mikroskopie elektronová rastrovací MeSH
- myši MeSH
- polymery * chemie MeSH
- povrchové vlastnosti MeSH
- proliferace buněk MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- polymery * MeSH
The precise design of bioactive surfaces, essential for the advancement of many biomedical applications, depends on achieving control of the surface architecture as well as on the ability to attach bioreceptors to antifouling surfaces. Herein, we report a facile avenue toward hierarchically structured antifouling polymer brushes of oligo(ethylene glycol) methacrylates via surface-initiated atom transfer radical polymerization (SI-ATRP) presenting photoactive tetrazole moieties, which permitted their functionalization via nitrile imine-mediated tetrazole-ene cyclocloaddition (NITEC). A maleimide-functional ATRP initiator was photoclicked to the side chains of a brush enabling a subsequent polymerization of carboxybetaine acrylamide to generate a micropatterned graft-on-graft polymer architecture as evidenced by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Furthermore, the spatially resolved biofunctionalization of the tetrazole-presenting brushes was accessed by the photoligation of biotin-maleimide and subsequent binding of streptavidin. The functionalized brushes bearing streptavidin were able to resist the fouling from blood plasma (90% reduction with respect to bare gold). Moreover, they were employed to demonstrate a model biosensor by immobilization of a biotinylated antibody and subsequent capture of an antigen as monitored in real time by surface plasmon resonance.
- MeSH
- fotochemie MeSH
- fotoelektronová spektroskopie MeSH
- hmotnostní spektrometrie MeSH
- methakryláty chemie MeSH
- polyethylenglykoly chemie MeSH
- polymery chemie MeSH
- povrchové vlastnosti MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- methakryláty MeSH
- polyethylenglykoly MeSH
- polymery MeSH
The successful synthesis of hierarchically structured titanium silicalite-1 (TS-1) with large intracrystalline macropores by steam-assisted crystallisation of mesoporous silica particles is reported. The macropore topology was imaged in 3D by using electron tomography and synchrotron radiation-based ptychographic X-ray computed tomography, revealing interconnected macropores within the crystals accounting for about 30 % of the particle volume. The study of the macropore formation mechanism revealed that the mesoporous silica particles act as a sacrificial macropore template during the synthesis. Silicon-to-titanium ratio of the macroporous TS-1 samples was successfully tuned from 100 to 44. The hierarchically structured TS-1 exhibited high activity in the liquid phase epoxidation of 2-octene with hydrogen peroxide. The hierarchically structured TS-1 surpassed a conventional nano-sized TS-1 sample in terms of alkene conversion and showed comparable selectivity to the epoxide. The flexible synthesis route described here can be used to prepare hierarchical zeolites with improved mass transport properties for other selective oxidation reactions.
- Klíčová slova
- catalysis, crystallization, epoxidation, nanostructures, zeolites,
- Publikační typ
- časopisecké články MeSH
An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm2; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm2; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm2) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca2+ flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.
- Klíčová slova
- biomimetic, cardiomyogenesis, fluorescence signal, human-induced pluripotent stem cells, surfaces,
- MeSH
- biokompatibilní materiály chemie MeSH
- buněčná diferenciace * MeSH
- fluorescence * MeSH
- indukované pluripotentní kmenové buňky cytologie MeSH
- kardiomyocyty cytologie MeSH
- lidé MeSH
- polystyreny chemie MeSH
- proliferace buněk MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biokompatibilní materiály MeSH
- polystyreny MeSH
In this present investigation, aluminium (Al3+) fabricated 2-aminobenzene-1,4-dicarboxylic acid (ABDC) namely Al@ABDC metal organic frameworks (MOFs) was developed for defluoridation studies. The unique advantages of developed MOFs possess high selectivity, high porosity and enhanced surface area but the developed powder form of Al@ABDC MOFs has several limitations in field applications like slow filtration and column blockage. To prevail over these troubles, biopolymer namely chitosan (CS) supported Al@ABDC MOFs namely Al@ABDC-CS beads were developed for effective fluoride adsorption from water. The synthesized Al@ABDC-CS beads were employed for the retention of fluoride in batch level. The defluoridation capacities (DCs) of Al@ABDC MOFs and Al@ABDC-CS beads were found to be 4880 and 4900 mgF- kg-1 respectively. The influencing parameters of adsorption method namely agitation time, adsorbent dosage, initial fluoride concentration, pH, co-existing anions and temperature were exploit to get utmost defluoridation capacity (DC) of Al@ABDC-CS beads. The experimental data of Al@ABDC-CS beads have been evaluated utilizing Langmuir, Fruendlich and Dubinin-Radushkevich (D-R) isotherms. The defluoridation nature of Al@ABDC-CS beads was determined by the thermodynamic parameters. The order of reaction of Al@ABDC-CS beads was studied using various kinetic models. The regeneration and field water studies of Al@ABDC-CS beads were also carried out to check their reusability and suitability at field conditions.
- Klíčová slova
- Aluminium, Chitosan, Defluoridation, Field study, Metal organic frameworks, Regeneration,
- MeSH
- adsorpce MeSH
- chemické látky znečišťující vodu * MeSH
- chitosan * MeSH
- fluoridy MeSH
- hliník MeSH
- kinetika MeSH
- koncentrace vodíkových iontů MeSH
- porézní koordinační polymery * MeSH
- termodynamika MeSH
- voda MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chemické látky znečišťující vodu * MeSH
- chitosan * MeSH
- fluoridy MeSH
- hliník MeSH
- porézní koordinační polymery * MeSH
- voda MeSH
In this study, a hybrid Ag-ZnO nanostructured micro-filler was synthesized by the drop technique for used in plastic and medical industry. Furthermore, new antibacterial polymer nanocomposites comprising particles of Ag-ZnO up to 5 wt % and a blend of a thermoplastic polyolefin elastomer (TPO) with polypropylene were prepared using twin screw micro-compounder. The morphology and crystalline-phase structure of the hybrid Ag-ZnO nanostructured microparticles obtained was characterized by scanning electron microscopy and powder X-ray diffractometry. The specific surface area of this filler was investigated by means of nitrogen sorption via the Brunauer-Emmet-Teller method. A scanning electron microscope was used to conduct a morphological study of the polymer nanocomposites. Mechanical and electrical testing showed no adverse effects on the function of the polymer nanocomposites either due to the filler utilized or the given processing conditions, in comparison with the neat polymer matrix. The surface antibacterial activity of the compounded polymer nanocomposites was assessed against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538P, according to ISO 22196:2007 (E). All the materials at virtually every filler-loading level were seen to be efficient against both species of bacteria.
- Klíčová slova
- Ag-ZnO, antibacterial, hierarchical, nanocomposites, polypropylene, thermoplastic elastomers,
- Publikační typ
- časopisecké články MeSH
Taking SnO2 quantum dots with random orientation as a precursor, NaOH induces self-assembly of SnO2 dots to form the nanowires, side-by-side attachment of which generates hierarchically ordered structures. The multistep oriented attachment mechanism can help to describe the oriented assembly of big nanocrystals.
In this article, we report a series of functionalized polyacetylene-type networks formed by chain-growth insertion coordination polymerization in high internal phase emulsions (HIPEs). All polymerized HIPEs (polyHIPEs) contain a hierarchically structured, 3D-interconnected porous framework consisting of a micro-, meso- and macropore system, resulting in exceptionally high specific surface areas (up to 1055 m2·g-1) and total porosities of over 95%. The combination of π-conjugated and hierarchically porous structure in one material enabled the use of these polyacetylene polyHIPEs as adsorptive photocatalysts for the removal of chemical contaminants from water. All polyacetylene polyHIPEs demonstrated high efficiency in the adsorption of bisphenol A from water (up to 48%) and the subsequent photocatalytic degradation. Surprisingly, high adsorption capacity did not affect the photocatalytic efficiency (up to 58%). On the contrary, this dual function seems to be very promising, as some polyacetylene polyHIPEs almost completely removed bisphenol A from water (97%) through the adsorption-photooxidation mechanism. It also appears that the presence of polar functional side groups in the polyacetylene backbone improves the contact of the polyacetylene network with the aqueous bisphenol A solution, which can thus be more easily adsorbed and subsequently oxidized, compensating for the lower specific surface area of some networks, namely, 471 and 308 m2·g-1 in the case of 3-ethynylphenol- and 3-ethynylaniline-based polyacetylene polyHIPEs, respectively.
- Publikační typ
- časopisecké články MeSH
Hybrid zones between divergent populations sieve genomes into blocks that introgress across the zone, and blocks that do not, depending on selection between interacting genes. Consistent with Haldane's rule, the Y chromosome has been considered counterselected and hence not to introgress across the European house mouse hybrid zone. However, recent studies detected massive invasion of M. m. musculus Y chromosomes into M. m. domesticus territory. To understand mechanisms facilitating Y spread, we created 31 recombinant lines from eight wild-derived strains representing four localities within the two mouse subspecies. These lines were reciprocally crossed and resulting F1 hybrid males scored for five phenotypic traits associated with male fitness. Molecular analyses of 51 Y-linked SNPs attributed ~50% of genetic variation to differences between the subspecies and 8% to differentiation within both taxa. A striking proportion, 21% (frequencies of sperm head abnormalities) and 42% (frequencies of sperm tail dissociations), of phenotypic variation was explained by geographic Y chromosome variants. Our crossing design allowed this explanatory power to be examined across a hierarchical scale from subspecific to local intrastrain effects. We found that divergence and variation were expressed diversely in different phenotypic traits and varied across the whole hierarchical scale. This finding adds another dimension of complexity to studies of Y introgression not only across the house mouse hybrid zone but potentially also in other contact zones.
- Klíčová slova
- Mus musculus domesticus, Mus musculus musculus, Y‐associated effects, phenotype variation, sperm quality, wild‐derived strain,
- Publikační typ
- časopisecké články MeSH
Many chemical compositions produce layered solids consisting of extended sheets with thickness not greater than a few nanometers. The layers are weakly bonded together in a crystal and can be modified into various nanoarchitectures including porous hierarchical structures. Several classes of 2-dimensional (2D) materials have been extensively studied and developed because of their potential usefulness as catalysts and sorbents. They are discussed in this review with focus on clays, layered transition metal oxides, silicates, layered double hydroxides, metal(iv) phosphates and phosphonates, especially zirconium, and zeolites. Pillaring and delamination are the primary methods for structural modification and pore tailoring. The reported approaches are described and compared for the different classes of materials. The methods of characterization include identification by X-ray diffraction and microscopy, pore size analysis and activity assessment by IR spectroscopy and catalytic testing. The discovery of layered zeolites was a fundamental breakthrough that created unprecedented opportunities because of (i) inherent strong acid sites that make them very active catalytically, (ii) porosity through the layers and (iii) bridging of 2D and 3D structures. Approximately 16 different types of layered zeolite structures and modifications have been identified as distinct forms. It is also expected that many among the over 200 recognized zeolite frameworks can produce layered precursors. Additional advances enabled by 2D zeolites include synthesis of layered materials by design, hierarchical structures obtained by direct synthesis and top-down preparation of layered materials from 3D frameworks.
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
