A microtubule-interacting protein involved in coalignment of vimentin intermediate filaments with microtubules
Language English Country Great Britain, England Media print
Document type Journal Article
PubMed
7907338
DOI
10.1242/jcs.106.4.1263
Knihovny.cz E-resources
- MeSH
- Antigens immunology metabolism MeSH
- 3T3 Cells MeSH
- Demecolcine pharmacology MeSH
- Fluorescent Antibody Technique MeSH
- Cell Compartmentation drug effects MeSH
- Actin Cytoskeleton metabolism MeSH
- Microinjections MeSH
- Microtubules metabolism MeSH
- Antibodies, Monoclonal MeSH
- Mice MeSH
- Paclitaxel pharmacology MeSH
- Microtubule-Associated Proteins immunology isolation & purification metabolism MeSH
- Vimentin metabolism MeSH
- Vinblastine pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antigens MeSH
- Demecolcine MeSH
- Antibodies, Monoclonal MeSH
- Paclitaxel MeSH
- Microtubule-Associated Proteins MeSH
- Vimentin MeSH
- Vinblastine MeSH
A protein of M(r) 210,000 was identified in 3T3 cells by immunoblotting and by immunoprecipitation with a monoclonal antibody MA-01. The protein was thermolabile and was located on 3T3 microtubules prepared by taxol-driven polymerization in vitro. On fixed cells the MA-01 antigen was located on interphase and mitotic microtubular structures, vinblastine paracrystals, taxol bundles and colcemid-resistant microtubules. Microinjection experiments with purified MA-01 antibody followed by double immunofluorescence have shown that the injection of antibody led to disruption of vimentin filaments, whereas the distribution of cytoplasmic microtubules was unchanged. The collapse of vimentin filaments started 30 minutes after injecting the antibody at immunoglobulin concentrations 2 mg ml-1 or higher and reached its maximum 3-6 hours after the injection. Within 20 hours after the injection vimentin filaments became reconstituted. Microinjection of the antibody into cells pre-treated with vinblastine resulted in localization of the MA-01 antigen on vinblastine paracrystals as well as on coiled vimentin filaments. The data presented suggest that the MA-01 antigen is a new microtubule-interacting protein that mediates, directly or indirectly, an interaction between microtubules and vimentin intermediate filaments.
References provided by Crossref.org
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