Trichomonas hydrogenosomes contain the NADH dehydrogenase module of mitochondrial complex I
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
PubMed
15577909
DOI
10.1038/nature03149
PII: nature03149
Knihovny.cz E-resources
- MeSH
- Aerobiosis MeSH
- Anaerobiosis MeSH
- Models, Biological MeSH
- Phylogeny MeSH
- Malates metabolism MeSH
- Mitochondria enzymology metabolism MeSH
- Molecular Sequence Data MeSH
- NAD metabolism MeSH
- NADH Dehydrogenase chemistry metabolism MeSH
- Organelles enzymology metabolism MeSH
- Protein Subunits chemistry metabolism MeSH
- Protozoan Proteins chemistry metabolism MeSH
- Electron Transport Complex I chemistry metabolism MeSH
- Amino Acid Sequence MeSH
- Sequence Alignment MeSH
- Symbiosis MeSH
- Trichomonas vaginalis cytology enzymology metabolism MeSH
- Hydrogen metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- Malates MeSH
- malic acid MeSH Browser
- NAD MeSH
- NADH Dehydrogenase MeSH
- Protein Subunits MeSH
- Protozoan Proteins MeSH
- Electron Transport Complex I MeSH
- Hydrogen MeSH
Hydrogenosomes are double-membraned ATP-producing and hydrogen-producing organelles of diverse anaerobic eukaryotes. In some versions of endosymbiotic theory they are suggested to be homologues of mitochondria, but alternative views suggest they arose from an anaerobic bacterium that was distinct from the mitochondrial endosymbiont. Here we show that the 51-kDa and 24-kDa subunits of the NADH dehydrogenase module in complex I, the first step in the mitochondrial respiratory chain, are active in hydrogenosomes of Trichomonas vaginalis. Like mitochondrial NADH dehydrogenase, the purified Trichomonas enzyme can reduce a variety of electron carriers including ubiquinone, but unlike the mitochondrial enzyme it can also reduce ferredoxin, the electron carrier used for hydrogen production. The presence of NADH dehydrogenase solves the long-standing conundrum of how hydrogenosomes regenerate NAD+ after malate oxidation. Phylogenetic analyses show that the Trichomonas 51-kDa homologue shares common ancestry with the mitochondrial enzyme. Recruitment of complex I subunits into a H2-producing pathway provides evidence that mitochondria and hydrogenosomes are aerobic and anaerobic homologues of the same endosymbiotically derived organelle.
References provided by Crossref.org
The retromer and retriever systems are conserved and differentially expanded in parabasalids
A hybrid TIM complex mediates protein import into hydrogenosomes of Trichomonas vaginalis
Reduced mitochondria provide an essential function for the cytosolic methionine cycle
Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis
Dynamic secretome of Trichomonas vaginalis: Case study of β-amylases
Arginine deiminase pathway enzymes: evolutionary history in metamonads and other eukaryotes
Iron-induced changes in the proteome of Trichomonas vaginalis hydrogenosomes
Live imaging of mitosomes and hydrogenosomes by HaloTag technology
The minimal proteome in the reduced mitochondrion of the parasitic protist Giardia intestinalis
Flavodiiron protein from Trichomonas vaginalis hydrogenosomes: the terminal oxygen reductase
Frataxin, a conserved mitochondrial protein, in the hydrogenosome of Trichomonas vaginalis
GENBANK
AY753550, AY753551