Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16254244
DOI
10.1242/jcs.02621
PII: 118/21/5035
Knihovny.cz E-resources
- MeSH
- Apoptosis drug effects MeSH
- Cell Nucleus drug effects enzymology genetics metabolism MeSH
- Cell Line MeSH
- Cell Cycle drug effects MeSH
- HT29 Cells MeSH
- Chromatin metabolism MeSH
- Chromosomal Proteins, Non-Histone antagonists & inhibitors metabolism MeSH
- Histone Deacetylases metabolism MeSH
- Histones metabolism MeSH
- Chromobox Protein Homolog 5 MeSH
- Enzyme Inhibitors pharmacology MeSH
- Histone Deacetylase Inhibitors * MeSH
- Interphase drug effects MeSH
- Butyric Acid pharmacology MeSH
- Hydroxamic Acids pharmacology MeSH
- Humans MeSH
- Carcinoma, Small Cell enzymology metabolism pathology MeSH
- Cell Line, Tumor MeSH
- Lung Neoplasms enzymology metabolism pathology MeSH
- Colonic Neoplasms enzymology metabolism pathology MeSH
- Fetus MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromatin MeSH
- Chromosomal Proteins, Non-Histone MeSH
- Histone Deacetylases MeSH
- Histones MeSH
- Chromobox Protein Homolog 5 MeSH
- Enzyme Inhibitors MeSH
- Histone Deacetylase Inhibitors * MeSH
- Butyric Acid MeSH
- Hydroxamic Acids MeSH
- trichostatin A MeSH Browser
The effects of the histone deacetylase inhibitors (HDACi) trichostatin A (TSA) and sodium butyrate (NaBt) were studied in A549, HT29 and FHC human cell lines. Global histone hyperacetylation, leading to decondensation of interphase chromatin, was characterized by an increase in H3(K9) and H3(K4) dimethylation and H3(K9) acetylation. The levels of all isoforms of heterochromatin protein, HP1, were reduced after HDAC inhibition. The observed changes in the protein levels were accompanied by changes in their interphase patterns. In control cells, H3(K9) acetylation and H3(K4) dimethylation were substantially reduced to a thin layer at the nuclear periphery, whereas TSA and NaBt caused the peripheral regions to become intensely acetylated at H3(K9) and dimethylated at H3(K4). The dispersed pattern of H3(K9) dimethylation was stable even at the nuclear periphery of HDACi-treated cells. After TSA and NaBt treatment, the HP1 proteins were repositioned more internally in the nucleus, being closely associated with interchromatin compartments, while centromeric heterochromatin was relocated closer to the nuclear periphery. These findings strongly suggest dissociation of HP1 proteins from peripherally located centromeres in a hyperacetylated and H3(K4) dimethylated environment. We conclude that inhibition of histone deacetylases caused dynamic reorganization of chromatin in parallel with changes in its epigenetic modifications.
References provided by Crossref.org
Cell differentiation and aging accompanied by depletion of the ACE2 protein
SUV39h-independent association of HP1 beta with fibrillarin-positive nucleolar regions
Histone modifications and nuclear architecture: a review
Single-cell c-myc gene expression in relationship to nuclear domains
