Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18339416
DOI
10.1016/j.virol.2008.01.010
PII: S0042-6822(08)00020-2
Knihovny.cz E-resources
- MeSH
- Ticks virology MeSH
- Encephalitis, Tick-Borne mortality physiopathology virology MeSH
- Animals, Suckling MeSH
- Models, Molecular MeSH
- Brain virology MeSH
- Mutation * MeSH
- Mice, Inbred ICR MeSH
- Mice MeSH
- RNA Helicases chemistry genetics MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Selection, Genetic MeSH
- Serine Endopeptidases chemistry genetics MeSH
- Serial Passage MeSH
- Amino Acid Substitution MeSH
- Viral Nonstructural Proteins chemistry genetics MeSH
- Virulence MeSH
- Encephalitis Viruses, Tick-Borne genetics isolation & purification pathogenicity MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Europe MeSH
- Names of Substances
- NS2B protein, flavivirus MeSH Browser
- NS3 protein, flavivirus MeSH Browser
- RNA Helicases MeSH
- Serine Endopeptidases MeSH
- Viral Nonstructural Proteins MeSH
An attenuated strain (263) of the tick-borne encephalitis virus, isolated from field ticks, was either serially subcultured, 5 times in mice, or at 40 degrees C in PS cells, producing 2 independent strains, 263-m5 and 263-TR with identical genomes; both strains exhibited increased plaque size, neuroinvasiveness and temperature-resistance. Sequencing revealed two unique amino acid substitutions, one mapping close to the catalytic site of the viral protease. These observations imply that virus adaptation from ticks to mammals occurs by selection of pre-existing virulent variants from the quasispecies population rather than by the emergence of new random mutations. The significance of these observations is discussed.
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History of Arbovirus Research in the Czech Republic
