Sequencing-based molecular typing of treponema pallidum strains in the Czech Republic: all identified genotypes are related to the sequence of the SS14 strain
Language English Country Sweden Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22434073
DOI
10.2340/00015555-1335
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- Drug Resistance, Bacterial genetics MeSH
- DNA, Bacterial analysis MeSH
- Adult MeSH
- Phenotype MeSH
- Genetic Variation MeSH
- Genotype MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Ribotyping * methods MeSH
- RNA, Ribosomal, 23S genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Syphilis diagnosis drug therapy epidemiology microbiology MeSH
- Treponema pallidum drug effects genetics immunology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- DNA, Bacterial MeSH
- RNA, Ribosomal, 23S MeSH
A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes.
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