Arabidopsis exocyst subcomplex containing subunit EXO70B1 is involved in autophagy-related transport to the vacuole
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23944713
DOI
10.1111/tra.12101
Knihovny.cz E-resources
- Keywords
- Arabidopsis, EXO70, EXO70B1, Golgi-independent, autophagy, exocyst, hypersensitive response, salicylic acid,
- MeSH
- Anthocyanins metabolism MeSH
- Arabidopsis metabolism MeSH
- Autophagy * MeSH
- Nitrogen metabolism MeSH
- Salicylic Acid metabolism MeSH
- Mutation MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Protein Transport MeSH
- Vacuoles metabolism MeSH
- Vesicular Transport Proteins genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anthocyanins MeSH
- Nitrogen MeSH
- EXO70B1 protein, Arabidopsis MeSH Browser
- Salicylic Acid MeSH
- NPR1 protein, Arabidopsis MeSH Browser
- Arabidopsis Proteins MeSH
- Vesicular Transport Proteins MeSH
Autophagic transport to the vacuole represents an endomembrane trafficking route, which is widely used in plants, not only during stress situations, but also for vacuole biogenesis and during developmental processes. Here we report a role in autophagic membrane transport for EXO70B1--one of 23 paralogs of Arabidopsis EXO70 exocyst subunits. EXO70B1 positive compartments are internalized into the central vacuole and co-localize with autophagosomal marker ATG8f. This internalization is boosted by induction of autophagy. Loss of function (LOF) mutations in exo70B1 cause reduction of internalized autopagic bodies in the vacuole. Mutant plants also show ectopic hypersensitive response (HR) mediated by salicylic acid (SA) accumulation, increased nitrogen starvation susceptibility and anthocyanin accumulation defects. Anthocyanin accumulation defect persists in npr1x exo70B1 double mutants with SA signaling compromised, while ectopic HR is suppressed. EXO70B1 interacts with SEC5 and EXO84 and forms an exocyst subcomplex involved in autophagy-related, Golgi-independent membrane traffic to the vacuole. We show that EXO70B1 is functionally completely different from EXO70A1 exocyst subunit and adopted a specific role in autophagic transport.
References provided by Crossref.org
ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants
A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley
Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit
Functional Specialization within the EXO70 Gene Family in Arabidopsis
EXO70A2 Is Critical for Exocyst Complex Function in Pollen Development
Evolution of late steps in exocytosis: conservation and specialization of the exocyst complex
Exocyst Subunit EXO70H4 Has a Specific Role in Callose Synthase Secretion and Silica Accumulation
Early Arabidopsis root hair growth stimulation by pathogenic strains of Pseudomonas syringae
RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane
EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth of Pollen
Tethering Complexes in the Arabidopsis Endomembrane System
Constitutive Negative Regulation of R Proteins in Arabidopsis also via Autophagy Related Pathway?
Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis
