Tetracycline-inducible gene expression system in Leishmania mexicana
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25461484
DOI
10.1016/j.molbiopara.2014.11.002
PII: S0166-6851(14)00166-2
Knihovny.cz E-resources
- Keywords
- Gene expression, Leishmania mexicana, Tet-inducible system,
- MeSH
- Gene Expression * MeSH
- Leishmania mexicana drug effects genetics metabolism MeSH
- Leishmaniasis, Cutaneous parasitology MeSH
- Humans MeSH
- Protozoan Proteins genetics metabolism MeSH
- Tetracycline pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Protozoan Proteins MeSH
- Tetracycline MeSH
Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.
References provided by Crossref.org
Distribution and Functional Analysis of Isocitrate Dehydrogenases across Kinetoplastids
Elimination of LRVs Elicits Different Responses in Leishmania spp
Catalase impairs Leishmania mexicana development and virulence
CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1
Trypanosome Mitochondrial Translation and Tetracycline: No Sweat about Tet
