Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.

Biology Centre Institute of Parasitology Czech Academy of Sciences 370 05 České Budějovice Czech Republic; Canadian Institute for Advanced Research Toronto ON M5G 1Z8 Canada

Life Science Research Centre Faculty of Science University of Ostrava 710 00 Ostrava Czech Republic

Life Science Research Centre Faculty of Science University of Ostrava 710 00 Ostrava Czech Republic; Biology Centre Institute of Parasitology Czech Academy of Sciences 370 05 České Budějovice Czech Republic

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