Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics
Language English Country Japan Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26738547
DOI
10.1093/pcp/pcv209
PII: pcv209
Knihovny.cz E-resources
- Keywords
- Arabidopsis thaliana, Confocal microscopy, Cotyledon pavement cells, Cytoskeleton, Formin, Variable angle epifluorescence microscopy,
- MeSH
- Actins metabolism MeSH
- Arabidopsis cytology drug effects metabolism MeSH
- Biomarkers metabolism MeSH
- Models, Biological MeSH
- Cytoskeleton drug effects metabolism MeSH
- Fluorescence MeSH
- Formins MeSH
- Clathrin metabolism MeSH
- Cotyledon drug effects metabolism MeSH
- Membrane Proteins metabolism MeSH
- Actin Cytoskeleton drug effects metabolism MeSH
- Microtubules drug effects metabolism MeSH
- Mutation genetics MeSH
- Arabidopsis Proteins metabolism MeSH
- Seedlings drug effects growth & development metabolism MeSH
- Thiones pharmacology MeSH
- Cell Shape * drug effects MeSH
- Uracil analogs & derivatives pharmacology MeSH
- Green Fluorescent Proteins metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- AFH1 protein, Arabidopsis MeSH Browser
- Actins MeSH
- Biomarkers MeSH
- Formins MeSH
- Clathrin MeSH
- Membrane Proteins MeSH
- Arabidopsis Proteins MeSH
- SMIFH2 compound MeSH Browser
- Thiones MeSH
- Uracil MeSH
- Green Fluorescent Proteins MeSH
Plant cell morphogenesis involves concerted rearrangements of microtubules and actin microfilaments. We previously reported that FH1, the main Arabidopsis thaliana housekeeping Class I membrane-anchored formin, contributes to actin dynamics and microtubule stability in rhizodermis cells. Here we examine the effects of mutations affecting FH1 (At3g25500) on cell morphogenesis and above-ground organ development in seedlings, as well as on cytoskeletal organization and dynamics, using a combination of confocal and variable angle epifluorescence microscopy with a pharmacological approach. Homozygous fh1 mutants exhibited cotyledon epinasty and had larger cotyledon pavement cells with more pronounced lobes than the wild type. The pavement cell shape alterations were enhanced by expression of the fluorescent microtubule marker GFP-microtubule-associated protein 4 (MAP4). Mutant cotyledon pavement cells exhibited reduced density and increased stability of microfilament bundles, as well as enhanced dynamics of microtubules. Analogous results were also obtained upon treatments with the formin inhibitor SMIFH2 (small molecule inhibitor of formin homology 2 domains). Pavement cell shape in wild-type (wt) and fh1 plants in some situations exhibited a differential response towards anti-cytoskeletal drugs, especially the microtubule disruptor oryzalin. Our observations indicate that FH1 participates in the control of microtubule dynamics, possibly via its effects on actin, subsequently influencing cell morphogenesis and macroscopic organ development.
References provided by Crossref.org
Transmembrane formins as active cargoes of membrane trafficking
Analysis of formin functions during cytokinesis using specific inhibitor SMIFH2
SH3Ps-Evolution and Diversity of a Family of Proteins Engaged in Plant Cytokinesis
Formins: linking cytoskeleton and endomembranes in plant cells
