Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics
Jazyk angličtina Země Japonsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26738547
DOI
10.1093/pcp/pcv209
PII: pcv209
Knihovny.cz E-zdroje
- Klíčová slova
- Arabidopsis thaliana, Confocal microscopy, Cotyledon pavement cells, Cytoskeleton, Formin, Variable angle epifluorescence microscopy,
- MeSH
- aktiny metabolismus MeSH
- Arabidopsis cytologie účinky léků metabolismus MeSH
- biologické markery metabolismus MeSH
- biologické modely MeSH
- cytoskelet účinky léků metabolismus MeSH
- fluorescence MeSH
- forminy MeSH
- klathrin metabolismus MeSH
- kotyledon účinky léků metabolismus MeSH
- membránové proteiny metabolismus MeSH
- mikrofilamenta účinky léků metabolismus MeSH
- mikrotubuly účinky léků metabolismus MeSH
- mutace genetika MeSH
- proteiny huseníčku metabolismus MeSH
- semenáček účinky léků růst a vývoj metabolismus MeSH
- thioketony farmakologie MeSH
- tvar buňky * účinky léků MeSH
- uracil analogy a deriváty farmakologie MeSH
- zelené fluorescenční proteiny metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- AFH1 protein, Arabidopsis MeSH Prohlížeč
- aktiny MeSH
- biologické markery MeSH
- forminy MeSH
- klathrin MeSH
- membránové proteiny MeSH
- proteiny huseníčku MeSH
- SMIFH2 compound MeSH Prohlížeč
- thioketony MeSH
- uracil MeSH
- zelené fluorescenční proteiny MeSH
Plant cell morphogenesis involves concerted rearrangements of microtubules and actin microfilaments. We previously reported that FH1, the main Arabidopsis thaliana housekeeping Class I membrane-anchored formin, contributes to actin dynamics and microtubule stability in rhizodermis cells. Here we examine the effects of mutations affecting FH1 (At3g25500) on cell morphogenesis and above-ground organ development in seedlings, as well as on cytoskeletal organization and dynamics, using a combination of confocal and variable angle epifluorescence microscopy with a pharmacological approach. Homozygous fh1 mutants exhibited cotyledon epinasty and had larger cotyledon pavement cells with more pronounced lobes than the wild type. The pavement cell shape alterations were enhanced by expression of the fluorescent microtubule marker GFP-microtubule-associated protein 4 (MAP4). Mutant cotyledon pavement cells exhibited reduced density and increased stability of microfilament bundles, as well as enhanced dynamics of microtubules. Analogous results were also obtained upon treatments with the formin inhibitor SMIFH2 (small molecule inhibitor of formin homology 2 domains). Pavement cell shape in wild-type (wt) and fh1 plants in some situations exhibited a differential response towards anti-cytoskeletal drugs, especially the microtubule disruptor oryzalin. Our observations indicate that FH1 participates in the control of microtubule dynamics, possibly via its effects on actin, subsequently influencing cell morphogenesis and macroscopic organ development.
Citace poskytuje Crossref.org
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SH3Ps-Evolution and Diversity of a Family of Proteins Engaged in Plant Cytokinesis
Formins: linking cytoskeleton and endomembranes in plant cells
