Organic radical imaging in plants: Focus on protein radicals
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30352303
DOI
10.1016/j.freeradbiomed.2018.10.428
PII: S0891-5849(18)31287-5
Knihovny.cz E-resources
- Keywords
- Alkoxyl radical, Alkyl radical, Hydroperoxide, Peroxyl radical, Photosystem, Reactive oxygen species,
- MeSH
- Cyclic N-Oxides chemistry MeSH
- Electron Spin Resonance Spectroscopy MeSH
- Lipids chemistry isolation & purification MeSH
- Oxidation-Reduction MeSH
- Oxidative Stress drug effects MeSH
- Hydrogen Peroxide chemistry MeSH
- Peroxides chemistry MeSH
- Proteins chemistry MeSH
- Reactive Oxygen Species chemistry MeSH
- Spin Trapping * MeSH
- Spin Labels MeSH
- Free Radicals chemistry isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cyclic N-Oxides MeSH
- Lipids MeSH
- Hydrogen Peroxide MeSH
- Peroxides MeSH
- Proteins MeSH
- Reactive Oxygen Species MeSH
- Spin Labels MeSH
- Free Radicals MeSH
Biomolecule (lipid and protein) oxidation products formed in plant cells exposed to photooxidative stress play a crucial role in the retrograde signaling and oxidative damage. The oxidation of biomolecules initiated by reactive oxygen species is associated with formation of organic (alkyl, peroxyl and alkoxyl) radicals. Currently, there is no selective and sensitive technique available for the detection of organic radicals in plant cells. Here, based on the analogy with animal cells, immuno-spin trapping using spin trap, 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was used to image organic radicals in Arabidopsis leaves exposed to high light. Using antibody raised against the DMPO nitrone adduct conjugated with the fluorescein isothiocyanate, organic radicals were imaged by confocal laser scanning microscopy. Organic radicals are formed predominantly in the chloroplasts located at the periphery of the cells and distributed uniformly throughout the grana stack. Characterization of protein radicals by standard immunological techniques using anti-DMPO antibody shows protein bands with apparent molecular weights of 32 and 34 kDa assigned to D1 and D2 proteins and two protein bands below the D1/D2 band with apparent molecular weights of 23 and 18 kDa and four protein bands above the D1/D2 band with apparent molecular weights of 41, 43, 55 and 68 kDa. In summary, imaging of organic radicals by immuno-spin trapping represents selective and sensitive technique for the detection of organic radicals that might help to clarify mechanistic aspects on the role of organic radicals in the retrograde signaling and oxidative damage in plant cell.
References provided by Crossref.org
Free Radical-Mediated Protein Radical Formation in Differentiating Monocytes
Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II
Characterization of Protein Radicals in Arabidopsis
Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana
Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana
