Structural and biochemical characterization of the novel serpin Iripin-5 from Ixodes ricinus
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
CZ.02.1.01/0.0/0.0/15_003/00004
European Regional Development Fund, MEYS
19-14704Y
Grantová Agentura České Republiky
105/2019/P
Jihočeská Univerzita v Českých Budějovicích
04-039/2019/P
Jihočeská Univerzita v Českých Budějovicích
PubMed
34473088
PubMed Central
PMC8573701
DOI
10.1107/s2059798321007920
PII: S2059798321007920
Knihovny.cz E-zdroje
- Klíčová slova
- Iripin-5, Ixodes ricinus, X-ray structure, serine protease inhibitors, serpins, tick saliva,
- MeSH
- antiflogistika * chemie izolace a purifikace MeSH
- erytrocyty MeSH
- inhibitory enzymů * chemie izolace a purifikace MeSH
- klíště metabolismus MeSH
- králíci MeSH
- kultivované buňky MeSH
- makrofágy MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- neutrofily MeSH
- serpiny * chemie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antiflogistika * MeSH
- inhibitory enzymů * MeSH
- serpiny * MeSH
Iripin-5 is the main Ixodes ricinus salivary serpin, which acts as a modulator of host defence mechanisms by impairing neutrophil migration, suppressing nitric oxide production by macrophages and altering complement functions. Iripin-5 influences host immunity and shows high expression in the salivary glands. Here, the crystal structure of Iripin-5 in the most thermodynamically stable state of serpins is described. In the reactive-centre loop, the main substrate-recognition site of Iripin-5 is likely to be represented by Arg342, which implies the targeting of trypsin-like proteases. Furthermore, a computational structural analysis of selected Iripin-5-protease complexes together with interface analysis revealed the most probable residues of Iripin-5 involved in complex formation.
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