Structural and biochemical characterization of the novel serpin Iripin-5 from Ixodes ricinus

. 2021 Sep 01 ; 77 (Pt 9) : 1183-1196. [epub] 20210823

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid34473088

Grantová podpora
CZ.02.1.01/0.0/0.0/15_003/00004 European Regional Development Fund, MEYS
19-14704Y Grantová Agentura České Republiky
105/2019/P Jihočeská Univerzita v Českých Budějovicích
04-039/2019/P Jihočeská Univerzita v Českých Budějovicích

Iripin-5 is the main Ixodes ricinus salivary serpin, which acts as a modulator of host defence mechanisms by impairing neutrophil migration, suppressing nitric oxide production by macrophages and altering complement functions. Iripin-5 influences host immunity and shows high expression in the salivary glands. Here, the crystal structure of Iripin-5 in the most thermodynamically stable state of serpins is described. In the reactive-centre loop, the main substrate-recognition site of Iripin-5 is likely to be represented by Arg342, which implies the targeting of trypsin-like proteases. Furthermore, a computational structural analysis of selected Iripin-5-protease complexes together with interface analysis revealed the most probable residues of Iripin-5 involved in complex formation.

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