The main problems of crop gene banks comprise heterogeneity of accessions, resulting from mechanical admixtures or out-crossing during their multiplication, and especially the mislabeling of accessions. These discrepancies can adversely affect the results of many expensive research and breeding projects that are based on the use of gene bank resources. To tackle these problems, 860 single-plant progenies (SPPs) of 172 accessions of the Czech winter barley core collection were grown and tested with a set of 53 isolates representing the global virulence/avirulence diversity of powdery mildew. Seventy-one resistance phenotypes encompassed the diversity of known specific resistances and their combinations. Based on testing groups of five SPPs, 94 accessions had one phenotype found in all five SPPs (homogeneous accessions), whereas in 78 accessions (45.3%) more than one phenotype was identified (heterogeneous accessions). In three varieties, specific resistances against the whole set of isolates were detected, but due to high adaptability of the pathogen, they are not recommended for breeding resistant cultivars. Selected SPPs were integrated in the gene bank and are now a reliable source of genotypically pure seed with defined powdery mildew resistance genes that can be used by breeders and researchers. The results obtained can be used to verify authenticity of accession genotype and pedigree, particularly for older varieties for which no other original criteria are available.

Department of Integrated Plant Protection Agrotest Fyto Ltd Havlíčkova 2787 CZ 767 01 Kroměříž Czech Republic

Gene Bank Crop Research Institute Drnovská 507 CZ 161 06 Praha 6 Ruzyně Czech Republic

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Non-Authenticity of Spring Barley Genotypes Revealed in Gene Bank Accessions

Postulation of Specific Disease Resistance Genes in Cereals: A Widely Used Method and Its Detailed Description

Powdery Mildew Resistance Genes in Single-Plant Progenies Derived from Accessions of a Winter Barley Core Collection