A chronic nonhealing wound poses a significant risk for infection and subsequent health complications, potentially endangering the patient's well-being. Therefore, effective wound dressings must meet several crucial criteria, including: (1) eliminating bacterial pathogen growth within the wound, (2) forming a barrier against airborne microbes, (3) promoting cell proliferation, (4) facilitating tissue repair. In this study, we synthesized 8 ± 3 nm Ag NP with maleic acid and incorporated them into an electrospun polycaprolactone (PCL) matrix with 1.6 and 3.4 µm fiber sizes. The Ag NPs were anchored to the matrix via electrospraying water-soluble poly(vinyl) alcohol (PVA), reducing the average sphere size from 750 to 610 nm in the presence of Ag NPs. Increasing the electrospraying time of Ag NP-treated PVA spheres demonstrated a more pronounced antibacterial effect. The resultant silver-based material exhibited 100% inhibition of gram-negative Escherichia coli and gram-positive Staphylococcus aureus growth within 6 h while showing non-cytotoxic effects on the Vero cell line. We mainly discuss the preparation method aspects of the membrane, its antibacterial properties, and cytotoxicity, suggesting that combining these processes holds promise for various medical applications.

Department of Physics Faculty of Science Humanities and Education Technical University of Liberec Studentská 5 461 17 Liberec Czech Republic

Department of Physics of Materials Faculty of Mathemathics and Physics Charles University Ke Karlovu 3 121 16 Prague Czech Republic

Faculty of Material Science and Technology Center for Advanced Innovation Technologies VSB Technical University of Ostrava Ostrava 17 listopadu 15 2172 708 00 Ostrava Czech Republic

Faculty of Materials Science and Technology FMT VSB Technical University of Ostrava 17 listopadu 2172 15 Poruba 708 00 Ostrava Czech Republic

Nanotechnology Centre CEET VSB Technical University of Ostrava 17 listopadu 15 2172 Poruba 708 00 Ostrava Czech Republic

National Institute for Nuclear Biological and Chemical Protection 5 V 1 Kamenná 71 262 31 Milín Czech Republic

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Sarviya N, Mahanta U, Dart A, et al. Biocompatible and antimicrobial multilayer fibrous polymeric wound dressing with optimally embedded silver nanoparticles. Appl. Surf. Sci. 2023;612:155799.

Krupová L, Pokorná A. Quality of life in patients with non-healing wounds, with particular focus on assesment tools: A literature review. Cent. Eur. J. Nurs. Midwifery. 2020;11:94–103.

Kus KJB, Ruiz ES. Wound dressings—A practical review. Curr. Dermatol. Rep. 2020;9:298–308.

Wang M, Zhao Q. Wound dressings—A practical review. Encycl. Biomed. Eng. 2018;1:1–15.

Mohseni M, Shamloo A, Aghababaie Z, et al. A comparative study of wound dressings loaded with silver sulfadiazine and silver nanoparticles: In vitro and in vivo evaluation. Int. J. Pharm. 2019;564:350–358. PubMed

Yilmaz AC, Aygin D. Honey dressing in wound treatment: A systematic review. Complement. Ther. Med. 2020;51:102388. PubMed

Hassiba AJ, El Zowalaty ME, Webster TJ, et al. Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications. Int. J. Nanomed. 2017;12:2205–2213. PubMed PMC

Wilkinson LJ, White RJ, Chipman JK. Silver and nanoparticles of silver in wound dressings: A review of efficacy and safety. J. Wound Care. 2011;20:543–549. PubMed

Lemraski EG, Alibeigi S, Abbasi Z. Ibuprofen@ silver loaded on poly (vinyl alcohol) / chitosan co-polymer scaffold as a novel drug delivery system. Mater. Today Commun. 2022;33:104311.

Mishra M, Ballal A, Rath D, et al. Colloids and surfaces B: Biointerfaces novel silver nanoparticle-antibiotic combinations as promising antibacterial and anti-biofilm candidates against multiple-antibiotic resistant ESKAPE microorganisms. Colloids Surf. B Biointerfaces. 2024;236:113826. PubMed

Xue CH, Chen J, Yin W, et al. Superhydrophobic conductive textiles with antibacterial property by coating fibers with silver nanoparticles. Appl. Surf. Sci. 2012;258:2468–2472.

Lee SH, Jun BH. Silver nanoparticles: Synthesis and application for nanomedicine. Int. J. Mol. Sci. 2019 PubMed PMC

Dong Y, Zhu H, Shen Y, et al. Antibacterial activity of silver nanoparticles of different particle size against vibrio natriegens. PLoS One. 2019;14:1–12. PubMed PMC

Anees Ahmad S, Sachi Das S, Khatoon A, et al. Bactericidal activity of silver nanoparticles: A mechanistic review. Mater. Sci. Energy Technol. 2020;3:756–769.

Pavlík V, Nešporová K, Sobotka L, et al. Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine. FASEB J. 2021;35:1–13. PubMed

Nešporová K, Pavlík V, Šafránková B, et al. Effects of wound dressings containing silver on skin and immune cells. Sci. Rep. 2020 PubMed PMC

Qin M, Liu D, Meng X, et al. Electrospun polyvinyl butyral/berberine membranes for antibacterial air filtration. Mater. Lett. X. 2021;10:100074.

Baskan H, Esentürk I, Dösler S, et al. Electrospun nanofibers of poly (acrylonitrile-co-itaconic acid)/silver and polyacrylonitrile/silver: In situ preparation, characterization, and antimicrobial activity. J. Ind. Text. 2021;50:1594–1624.

Repanas A, Andriopoulou S, Glasmacher B. The significance of electrospinning as a method to create fibrous scaffolds for biomedical engineering and drug delivery applications. J. Drug Deliv. Sci. Technol. 2016;31:137–146.

Asghari F, Samiei M, Adibkia K, et al. Biodegradable and biocompatible polymers for tissue engineering application: A review. Artif. Cells Nanomed. Biotechnol. 2016 PubMed

Tsiapla AR, Bakola V, Karagkiozaki V, et al. Biodegradable electrosprayed NPS as drug carriers for optimal treatment of orthopaedic infections. Mater. Today Proc. 2019;19:110–116.

Nguyen DN, Clasen C, Van den Mooter G. Pharmaceutical applications of electrospraying. J. Pharm. Sci. 2016;105:2601–2620. PubMed

Boda SK, Li X, Xie J. Electrospraying an enabling technology for pharmaceutical and biomedical applications: A review. J. Aerosol. Sci. 2018;125:164–181. PubMed PMC

Vilamová Z, Konvičková Z, Mikeš P, et al. Ag-AgCl nanoparticles fixation on electrospun PVA fibres: Technological concept and progress. Sci. Rep. 2019;9:1–10. PubMed PMC

Mochane MJ, Motsoeneng TS, Sadiku ER, et al. Morphology and properties of electrospun PCL and its composites for medical applications: A mini review. Appl. Sci. 2019;9:1–17.

Alt Z. Biointerfaces random/aligned electrospun PCL fibrous matrices with modified surface textures: Characterization and interactions with dermal fibroblasts and keratinocytes. Colloids Surf. B Biointerfaces. 2022 PubMed

Ogur E. Polyvinyl alcohol: Materials, processing and applications. Rapra Rev. Rep. 2005;16:1–130.

Guarino V, Gentile G, Sorrentino L, et al. Encyclopedia of Polymer Science and Technology. Wiley; 2017. Polycaprolactone: Synthesis, properties, and applications; pp. 1–36.

Azimi B, Nourpanah P, Rabiee M, et al. Poly (ε-caprolactone) fiber: An overview. J. Eng. Fibers Fabr. 2014;9:74–90.

Ghodake G, Shinde S, Kadam A, et al. Gallic acid-functionalized silver nanoparticles as colorimetric and spectrophotometric probe for detection of Al3+ in aqueous medium. J. Ind. Eng. Chem. 2020;82:243–253.

Chou HL, Wu CM, Lin FD, et al. Interactions between silver nanoparticles and polyvinyl alcohol nanofibers. AIP Adv. 2014

Issa A, Al-Maadeed M, Luyt A, et al. Physico–mechanical, dielectric, and piezoelectric properties of PVDF electrospun mats containing silver nanoparticles. C. 2017;3:30.

Sa B, Mukherjee S, Roy SK. Effect of polymer concentration and solution pH on viscosity affecting integrity of a polysaccharide coat of compression coated tablets. Int. J. Biol. Macromol. 2019;125:922–930. PubMed

Lee VC. The antibiotic resistance crisis. P&T Peer/Rev. J. Formul. Manag. 2015;40:77–283.

Li D, Liu Z, Yuan Y, et al. Green synthesis of gallic acid-coated silver nanoparticles with high antimicrobial activity and low cytotoxicity to normal cells. Process. Biochem. 2015;50:357–366.

Augustine R, Kalarikkal N, Thomas S. Electrospun PCL membranes incorporated with biosynthesized silver nanoparticles as antibacterial wound dressings. Appl. Nanosci. 2016;6:337–344.

Leonas KK, Jones CR. The relationship of fabric properties and bacterial filtration efficiency for selected surgical face masks. J. Text Appar. Technol. Manag. 2003;3:4–5.

Morina E, Dotter M, Döpke C, et al. Homogeneity of needleless electrospun nanofiber mats. Nanomaterials. 2023;13:1–11. PubMed PMC

Yunoki S, Kohta M, Ohyabu Y, et al. In vitro parallel evaluation of antibacterial activity and cytotoxicity of commercially available silver-containing wound dressings. Plast. Surg. Nurs. 2015;35:203–211. PubMed

Graves JL, Tajkarimi M, Cunningham Q, et al. Rapid evolution of silver nanoparticle resistance in Escherichia coli. Front. Genet. 2015;5:1–13. PubMed PMC

You C, Li Q, Wang X, et al. Silver nanoparticle loaded collagen/chitosan scaffolds promote wound healing via regulating fibroblast migration and macrophage activation. Sci. Rep. 2017;7:1–11. PubMed PMC

Sahu N, Soni D, Chandrashekhar B, et al. Synthesis of silver nanoparticles uusing flavonoids: Hesperidin, naringin and diosmin, and their antibacterial effects and cytotoxicity. Int. Nano Lett. 2016;6:173–181.

Li W, Zhou J, Xu Y. Study of the in vitro cytotoxicity testing of medical devices. Biomed. Rep. 2015;3:617–620. PubMed PMC

Siddiqui N, Asawa S, Birru B, et al. PCL-based composite scaffold matrices for tissue engineering applications. Mol. Biotechnol. 2018;60:506–532. PubMed