AIMS: Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood components, and subsequently to investigate their basic biological properties. METHODS: DPSC were cultivated in five different media based on α MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically. RESULTS: We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 ± 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 ± 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 ± 10.4 hours), 2% PRP (DT 40.1 ± 5.7 hours) and 2% HP (DT 49.0 ± 15.2 hours) showed almost the same proliferative activity. DPSC's viability in the 9th passage was over 90% except for the DPSC cultivated in the 10% HP media. CONCLUSIONS: We proved that human blood components are suitable substitution for FCS in cultivation media for long-term DPSC cultivation.

• MeSH
• buněčné kultury metody   MeSH
• čas MeSH
• fetální krev * MeSH
• kultivační média MeSH
• kultivované buňky MeSH
• lidé MeSH
• mezenchymální kmenové buňky * MeSH
• plazma bohatá na destičky * MeSH
• proliferace buněk MeSH
• skot MeSH
• zubní dřeň cytologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

AIMS: Authors studied potential side effects of fetal calf serum (FCS) in cultivation media on human dental pulp stem cells (DPSC) during long-term cultivation. METHODS: Two lines of DPSC obtained healthy donors (male 22 years, female 23 years) were used. Both lines were cultivated under standard cultivation conditions in four different media containing 10% or 2% FCS and substituted with growth factors. During long-term cultivation proliferation ability, karyotype and phenotype of DPSC were measured. RESULTS: Both lines of DPSC cultivated in a media containing 2% FCS and ITS supplement showed the highest number of population doublings. On the other hand the proliferation rate of DPSC cultivated in a media with 2% FCS without ITS supplement was slowest. Proliferation rate of DPSC cultivated in 10% FCS media with or without FGF-2 was comparable. DPSC cultivated in a media with 10% FCS showed a significantly higher amount of chromosomal aberrations. These chromosomal aberrations do not seem to be clonal but surprisingly we found large amounts of tetraploid cells in the 9th passage in both media containing 10% FCS. CONCLUSIONS: Our study proved that cultivation of DPSC in media containing higher concentration of FCS has critical side effects on cell chromosomal stability.

• MeSH
• fetální krev cytologie   MeSH
• kmenové buňky cytologie   MeSH
• kultivované buňky MeSH
• lidé MeSH
• mladý dospělý MeSH
• proliferace buněk MeSH
• skot MeSH
• zubní dřeň cytologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• skot MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

PURPOSE: To monitor the development of stability of immediately loaded implants during early healing. MATERIALS AND METHODS: A total of 90 interforaminally placed implants with an alkali-treated surface were considered. The stability of each implant was examined at placement and 1, 2, 3, 4, 5, 6, 8, and 10 weeks after the surgery using resonance frequency analysis (RFA) and damping capacity measurement. The development of implant stability, focusing on the decrease in stability (as measured by implant stability quotient [ISQ]) and the interplay of primary (ISQ0) and secondary implant stability, was evaluated. The implants were divided into three groups based on primary stability: group L (ISQ0 < 68), group M (ISQ0 68 to72), and group H (ISQ0 > 72). Stability curves for each group were created and analyzed statistically. Implant stability measurement results gained with RFA and damping capacity were compared employing the Wilcoxon paired test, correlation coefficients, and regression analysis. The threshold for statistical significance was set at P < .05. RESULTS: The most pronounced decrease in ISQ values occurred 1 week after implant placement (mean decrease of 2.2 ISQ). During the 10-week experiment, mean ISQ rose by 5.5 in group L and by 1.3 in group M and dropped by 1.8 in group H (P < .001). The coefficient of determination R2 = 0.06 showed a weak dependence of RFA on the damping capacity (P < .001). CONCLUSIONS: Implants with low primary stability showed a significant increase in stability during healing. In contrast, implants with high primary stability lost some stability over time.

• MeSH
• endoseální implantace zubů metody   MeSH
• hojení ran MeSH
• lidé středního věku MeSH
• lidé MeSH
• mandibula chirurgie   MeSH
• neparametrická statistika MeSH
• okamžité zatížení zubního implantátu MeSH
• osteointegrace MeSH
• povrchové vlastnosti MeSH
• prospektivní studie MeSH
• regresní analýza MeSH
• retence zubní protézy MeSH
• senioři MeSH
• vibrace MeSH
• zubní implantáty MeSH
• zubní protéza - design MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH

PURPOSE: To investigate the parameters that affect primary stability of dental implants, to determine how primary stability influences posthealing stability, and to ascertain the effect of primary stability and insertion parameters on marginal bone loss. MATERIALS AND METHODS: A total of 940 immediately loaded implants were considered. Using resonance frequency analysis, primary stability (primary implant stability quotient [pISQ]) and stability after 4 months (tISQ) were recorded. When the differences between pISQ and tISQ exceeded 5 units, marginal bone loss was measured. The implants were placed into three groups based on their primary stability: high (pISQ > 72), moderate, and low (pISQ < 68). Changes in stability after 4 months of loading were evaluated. The relationships between pISQ, insertion parameters, DISQ (ie, tISQ - pISQ), and marginal bone loss were analyzed. The Student t test, one-way analysis of variance, and Spearman nonparametric correlation coefficient were employed for statistical evaluation. RESULTS: Of the 940 implants, tISQ was recorded in 526 implants and marginal bone loss was measured in 76 implants. There was no statistical relationship between pISQ and insertion torque. Primary stability was influenced by implant diameter but not by implant length. There was a significant relationship between implant insertion torque and bone type. The low primary stability group showed a significant increase in stability during healing. However, high primary stability implants demonstrated a significant reduction in their stability. The linear regression analysis demonstrated that at a pISQ of 69.2, tISQ value would equal pISQ value. Correlations between marginal bone loss and final insertion torque and between marginal bone loss and DISQ values were observed. CONCLUSIONS: Stability of immediately loaded implants with high pISQ decreased significantly during the initial 4 months of healing. However, stability of implants with low primary stability increased significantly. DISQ and insertion torque showed correlation with marginal bone loss.

• MeSH
• analýza rozptylu MeSH
• čelist bezzubá rehabilitace   chirurgie   MeSH
• endoseální implantace zubů metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• longitudinální studie MeSH
• mandibula MeSH
• neparametrická statistika MeSH
• podpěry zubní MeSH
• resorpce alveolární kosti prevence a kontrola   MeSH
• retence zubní protézy škodlivé účinky   MeSH
• retrospektivní studie MeSH
• točivý moment MeSH
• výsledek terapie MeSH
• zatížení muskuloskeletálního systému MeSH
• zubní implantáty škodlivé účinky   MeSH
• zubní náhrada ve spojení s implantáty škodlivé účinky   MeSH
• zubní náhrady úplné dolní MeSH
• zubní protéza - design MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• srovnávací studie MeSH