We studied the surface properties and cytocompatibil-ity of a grafted biopolymer, poly(3-hydroxybutyrate) (P3HB). P3HB was exposed to an inert argon plasma dis-charge, then grafted by 1,1 '-biphenyl-4,4 '-dithiol (BFD) and finally grafted by golden nanoparticles (nanospheres and nanorods). The surface properties were studied using multiple methods – goniometry, atomic force microscopy and X-ray photoelectron spectroscopy. Cytocompatibility was determined in vitro by studying adhesion, prolifera-tion and viability of vascular smooth muscle cells (VSMCs) from the aorta of Rattus norvegicus. The cyto-compatibility was compared for pristine, modified P3HB and standard tissue culture polystyrene (TCPS). Our re-sults show that surface morphology and wettability are affected by both plasma discharge and nanoparticles graft-ing. These changes suggest that the adhesion and prolifera-tion of VSMCs is enhanced more on the plasma modified and grafted substrate.
This paper provides a brief insight into the world of nanotechnology and nanostructures, including a short excursus into their history, principles and related properties. Although there are many ways how to prepare nanostructures, this work describes only laser modification technique of polymers, which is still up to date for various potential applications. The study of the surface morphology by microscopic methods represents an integral part of the nanostructure research. The most significant of them are briefly described in this work
- MeSH
- lasery MeSH
- mikroskopie metody MeSH
- nanostruktury * MeSH
- polymery MeSH
- povrchové vlastnosti MeSH
- Publikační typ
- práce podpořená grantem MeSH
In this research, influence of storage conditions on properties of oxidized cellulose was studied with respect to its haemostatic function. The aim was to examine changes of the properties of oxidized cellulose stored properly and that stored at laboratory conditions for 2 years. We studied surface morphology and chemical composition, as well as absorption of the simulated body fluid, behaviour in aqueous environment via potentiometric measurement of pH, and antimicrobial activity in vitro on the S. epidermidis bacteria. It was found out that the material properties of oxidized cellulose did not deteriorate. Higher absorption of simulated body fluid, lower pH in water and simulated body fluid represented positive changes with respect to the haemostatic function. Due to the acidic nature of the mate-rial, degraded oxidized cellulose preserved its antibacterial properties.
- MeSH
- antibakteriální látky analýza MeSH
- celulosa oxidovaná * analýza MeSH
- elektronová mikroskopie metody MeSH
- fotoelektronová spektroskopie metody MeSH
- hemostatika analýza MeSH
- řízení kvality MeSH
- skladování léků MeSH
- vystavení vlivu životního prostředí prevence a kontrola MeSH
- Publikační typ
- klinická studie MeSH
- práce podpořená grantem MeSH
