Fibre type determination requires a large series of differently stained muscle sections. The manual identification of individual fibres through the series is tedious and time consuming. This paper presents a software that enables (i) adjusting the position of individual fibres through a series of differently stained sections (image registration) and identification of individual fibres through the series as well as (ii) muscle fibre classification and (iii) quantitative analysis. The data output of the system is the following: numerical and areal proportions of fibre types, fibre type size and optical density (grey level) of the final reaction product in every fibre. The muscle fibre type can be determined stepwise, based on one set of stained sections while further, newly stained sections can be added to the already defined muscle fibre profile. Several advantages of the presented software application in skeletal muscle research are presented. The system is semiquantitative, flexible, and user friendly.
- MeSH
- hybridizace in situ MeSH
- imunohistochemie MeSH
- kosterní svalová vlákna cytologie klasifikace MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- musculus masseter cytologie MeSH
- myosiny genetika metabolismus MeSH
- počítačové zpracování obrazu metody MeSH
- protein - isoformy genetika metabolismus MeSH
- reprodukovatelnost výsledků MeSH
- software MeSH
- těžké řetězce myosinu genetika metabolismus MeSH
- uživatelské rozhraní počítače MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
Young and adult uninephrectomized male rats (aged 25 and 87 days respectively) were exposed to an increased salt intake (1% saline as the only drinking fluid) either alone or in combination with DOCA-treatment for 25 and 46 days respectively. Age dependent differences of interrelationships between saline intake (SI), blood pressure (BP) and kidney weight (KW) were studied during development of salt and DOCA-salt hypertension to specify possible factors involved in the higher susceptibility of the young rats to these regimes. Correlation analysis was employed using the step-wise regression procedure. Only in the young rats did saline treatment induce an increase in KW, which preceded the development of mild hypertension. This age group also responded to DOCA-saline treatment with a more pronounced increase in both BP and KW. SI was higher in the young than adult rats exposed to either saline or DOCA-saline treatment. This, however, does not account by itself for the higher hypertensive response of the young rats, since there was no primary relationship between SI and BP in the hypertensive groups. Increase in KW accompanying development of hypertension was dependent on BP in the young rats and on SI in adult rats. This indicates that saline and DOCA-saline treatment renders the kidneys of young rats more sensitive to damaging effects of BP, which play a part in the more pronounced hypertensive response.
- MeSH
- deoxykortikosteron aplikace a dávkování škodlivé účinky MeSH
- homeostáza účinky léků MeSH
- hypertenze patofyziologie MeSH
- krevní tlak účinky léků MeSH
- krysa rodu rattus MeSH
- ledviny patofyziologie MeSH
- sodík aplikace a dávkování škodlivé účinky MeSH
- tělesná hmotnost MeSH
- věkové faktory MeSH
- velikost orgánu MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
