The aim of our study was to analyse the mitochondrial ultrastructure in primary ductal adenocarcinomas of the pancreas and to compare it with normal pancreatic cells. 52 samples of adenocarcinoma of the pancreas obtained by surgical resection or by endosonographic biopsy were examined. Compared to normal mitochondrial ultrastructure in non-tumorous cells, the mitochondria in cancer cells had a dense matrix and condensed configuration or with lucent-swelling matrix associated with disarrangement and distortion of cristae and partial or total cristolysis. Functionally, these structural alterations presume the presence of hypoxia-tolerant and hypoxia-sensitive cancer cells.
- Publikační typ
- abstrakt z konference MeSH
The objective of the study was to ascertain if the estradiol-progesterone substitution used for the preparation of endometrium which has been shown to elicit supraphysiological levels of circulating progesterone may also induce demonstrable changes in the ultrastructural morphology of human secretory endometrium which is characterised by three progesteron- dependent structures, the so called postovulatory triad: nuclear or nucleolar channel system, giant mitochondria and subnuclear glycogen. The study was approved by the institutional Ethic Committee and included 24 patients of one IVF centre who signed informed consent. Two sequentional endometrial biopsy specimens were taken on the 5th and 7th day after ovulation in one natural cycle and on the 5th and 7th day after the initiation of progesterone addition in another substituted cycles in the same patient. The surface and glandular epithelial cells were examined under transmission electron microscope (TEM). The appearance of nuclear channel system (NCS), giant mitochondria and glycogen was the same in both natural and substituted cycles, without abnormalities and deformations. Cells with NCS were found, mostly in clusters on discrete parts, in 55 to 70 % of ultrathin sections. The relative number of NCS positive specimens was not statistically different between the cycles or between the day +5 and the day +7 within different type of cycle. Some NCS positivity was detected more often (in 79 % and 66 %, resp.) in substituted cycles than in spontaneous ones. The percentage of NCS positive cells was also higher (median 17 % and 12 %, resp.) in substituted cycles but the differences were not statistically significant. With some caution regarding the inherent limited reproducibility of the TEM findings we can conclude that the oral hormonal substitution used in our setting does not markedly alter endometrial ultrastructural morphology.
Ultrastructure of the superficial relief of the endometrial epithelium was investigated under the scanning (SEM) and transmission electron microscopes (TEM) in a group of patients after the hormonal stimulation, performed in the program of in vitro fertilization and embryotransfer. The results were compared with those obtained in a group of patients studied during the secretory phase of normal menstrual cycle. Total 26 bioptic samples were subjected to the investigation, 13 in each group. Different types of the epithelial surface were classified in TEM and SEM and their relative amount was evaluated quantitatively in TEM. Special attention was paid to the pinopodes, considered as specific markers of the nidation window. Differences in the number of pinopodes were observed under the SEM in two groups of patients after different schemes of the hormonal treatment. These differences were not manifested under the TEM due to the small frequency of pinopodes in the ultrathin sections. The ultrastructural changes observed in the two groups of patients after different schemes of hormonal stimulation can be considered as signs of the changed metabolic activity of the endometrial epithelium and they can reflect the different endometrial receptivity for nidation.
- MeSH
- buněčná membrána účinky léků ultrastruktura MeSH
- choriogonadotropin farmakologie MeSH
- elektronová mikroskopie MeSH
- endometrium účinky léků fyziologie ultrastruktura MeSH
- epitelové buňky účinky léků fyziologie ultrastruktura MeSH
- folikuly stimulující hormon farmakologie MeSH
- klomifen farmakologie MeSH
- lidé MeSH
- menotropiny farmakologie MeSH
- menstruační cyklus * MeSH
- mikroskopie elektronová rastrovací MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The ultrastructure of NCS was investigated in two groups of patients (15 in each) during the secretory phase of the normal menstrual cycle and after the hormonal stimulation performed in the IVF programme by GnRH-a, FSH and hCG. Three developmental stages of the NCS were classified. The most numerous were the NCS in the mature stage (type 2), less in the early developmental stage (type 1) and least in the regression stage (type 3). The simultaneous occurrence of all three types does not conform the statement of Spornitz. The proportions of the three types were identical in both investigated groups of patients. The number of NCS (per 100 of nuclei) was higher in the group after hormonal stimulation than in the normal cycle. The quantitative measurement of the NCS showed its larger size in the group after hormonal stimulation than in the normal cycle, but the differences in the shape were not significant. In the serial ultrathin sections we demonstrated the relation of NCS to the nucleolus and to the invaginations of the nuclear envelope, the formation of the electron lucent center and the spiral arrangement of the tubules. In the early regression stage we described the relation of the internal row of tubules to the formed myelin figures. We suppose that the higher number and larger size of NCS can be taken as a sign of the increased activity of the endometrium during the early secretory phase after the hormonal stimulation.
- MeSH
- choriogonadotropin farmakologie MeSH
- endometrium účinky léků fyziologie ultrastruktura MeSH
- fertilizace in vitro MeSH
- folikuly stimulující hormon farmakologie MeSH
- hormon uvolňující gonadotropiny farmakologie MeSH
- jaderný obal účinky léků fyziologie ultrastruktura MeSH
- lidé MeSH
- menstruační cyklus MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
