- MeSH
- lidé MeSH
- paliativní medicína * výchova MeSH
- podpora školení * MeSH
- výchova a vzdělávání MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
- MeSH
- komunikace MeSH
- lidé MeSH
- paliativní péče * metody MeSH
- spiritualita MeSH
- vztahy mezi lékařem a pacientem MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- rozhovory MeSH
- MeSH
- lidé MeSH
- smrt MeSH
- vztahy mezi odborníkem a rodinou MeSH
- zármutek * MeSH
- ztráta blízké osoby MeSH
- Check Tag
- lidé MeSH
Long considered as ectopic breast tissue, anogenital mammary-like glands (MLGs) have recently been suggested to represent distinctive structures located in the anogenital area. We studied 16 neoplasms of anogenital MLG for human papillomavirus (HPV) DNA using INNO-line probe assay (LiPA) HPV Genotyping kit, GP5+/6+, CP(SGB), and FAP 6085-6319 primer sets. The lesions included 3 fibroadenomas, 2 adenosis tumors, 1 invasive ductal carcinoma, 1 tubulolobular carcinoma, 2 hidradenoma papilliferum with prominent cystic change rendering a cystadenoma appearance and oxyphilic metaplasia, and 7 cases of extramammary Paget disease. All 3 fibroadenomas, both adenosis tumors, both hidradenoma papilliferum, and the tubulolobular carcinoma proved negative for HPV DNA. HPV-31 was detected by LiPA in the case of invasive ductal carcinoma. In 2 of the 7 patients with extramammary Paget disease, there was HPV DNA present in the lesional tissue, typed as HPV-6 (LiPA) and a type which was closely related to HPV-21 and HPV-24 (FAP 6085-6319), whereas the remaining 5 cases tested negative. These results coupled with those obtained from literature review suggest that HPV plays no causative role in lesions of anogenital MLG.
- MeSH
- dospělí MeSH
- exokrinní žlázy patologie MeSH
- infekce onkogenními viry epidemiologie MeSH
- infekce papilomavirem epidemiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory anu virologie MeSH
- nádory ženských pohlavních orgánů virologie MeSH
- Papillomaviridae izolace a purifikace MeSH
- polymerázová řetězová reakce MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
The authors report 10 cases of lymphoepithelioma-like carcinoma (LELC) of the skin and the results of a molecular biological study for HPV, EBV, and SV40 in lesional tissues. All patients originated from Central Europe. There were seven men and three women, ranging in age from 57 to 86 years. Locations included the face (n = 4), scalp (n = 2), penis (n = 2), and retroauricular area (n = 1); location was unknown for one subject. All but two patients presented with a tumor confined to the skin; in both patients with the penile carcinoma, the tumors had metastasized to an inguinal lymph node. Six patients with available follow-up included four individuals with no evidence of tumor metastasis or recurrences at 2, 3, 4, and 5 years, one patient who died with metastatic disease 7 years after diagnosis, and one patient who died of an unrelated course. Microscopically, all cases showed distinctive features of LELC characterized by variably sized and shaped nodules or syncytial sheets of epithelial cells that contained vesicular chromatic and prominent nucleoli and that were permeated and surrounded by small, well-differentiated lymphocytes and plasma cells. Because all 10 cases studied proved negative for EBV, HPV, and SV40, these viruses seem to play no causal role in LELC of the skin in patients from Central Europe.
- MeSH
- Alphapapillomavirus izolace a purifikace MeSH
- buněčné jadérko ultrastruktura MeSH
- epitelové buňky patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfatické metastázy patologie MeSH
- lymfocyty patologie MeSH
- míra přežití MeSH
- nádory kůže virologie MeSH
- nádory obličeje virologie MeSH
- nádory penisu virologie MeSH
- nádory ucha virologie MeSH
- následné studie MeSH
- opičí virus SV40 izolace a purifikace MeSH
- plazmatické buňky patologie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- skalp virologie MeSH
- spinocelulární karcinom sekundární virologie MeSH
- virus Epsteinův-Barrové izolace a purifikace MeSH
- zevní ucho virologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Geografické názvy
- Česká republika MeSH
- Slovenská republika MeSH
The principal aims of this study were to test whether persistence of human papillomavirus (HPV) DNA is predictive of recurrent disease in women after surgical treatment for cervical lesions, to distinguish between persistent and newly acquired HPV infection, and to observe the effect of surgical treatment on levels of HPV-specific antibodies. A group of 198 patients surgically treated for low-grade and high-grade squamous intraepithelial lesions and 35 age-matched controls were monitored for 18 months at 6-month intervals. The presence of HPV DNA in cervical smears was detected by means of consensus polymerase chain reaction, and serum levels of HPV-specific antibodies to HPV types 16, 18, 31, 33, and 45 were measured. In ten patients positive for HPV type 16 in consecutive samples, the HPV 16 variants were identified using a polymerase chain reaction specific for the long control region. Data regarding demographics, risk factors for cervical cancer, and risks related to HPV exposure were collected through a patient questionnaire. Subjects persistently positive for HPV DNA were more likely to present with cytological and/or colposcopical abnormalities. A higher reactivity to HPV-specific antibodies was observed in these women at the 18-month follow-up visit. All ten patients with HPV 16 infection detected in consecutive samples showed persistence of either the same prototype or the same variant during the follow-up period. Risky sexual behavior and smoking were more common in patients than in controls. Persistent HPV infection as demonstrated by both HPV DNA detection and antibody detection appears to be a risk factor for the recurrence of pathological findings in women after surgery. An individually based approach to surgical treatment is an important factor in the outcome of disease at follow-up.
- MeSH
- DNA virů analýza MeSH
- dospělí MeSH
- financování organizované MeSH
- infekce onkogenními viry MeSH
- infekce papilomavirem epidemiologie imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- lidský papilomavirus 16 genetika imunologie izolace a purifikace MeSH
- longitudinální studie MeSH
- mladiství MeSH
- nádory děložního čípku chirurgie virologie MeSH
- následné studie MeSH
- nemoci cervix uteri chirurgie virologie MeSH
- Papillomaviridae genetika izolace a purifikace MeSH
- prevalence MeSH
- protilátky virové MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- senioři MeSH
- ženské pohlaví MeSH
- MeSH
- DNA virů * analýza MeSH
- dospělí MeSH
- epididymis * patologie virologie MeSH
- lidé MeSH
- metaplazie MeSH
- Papillomaviridae genetika izolace a purifikace MeSH
- polymerázová řetězová reakce MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- dopisy MeSH
- kazuistiky MeSH
- komentáře MeSH
