The objective of the study is to standardize GC determination of polyunsaturated fatty acids in animal tissues by lipid extraction with a hexane/propan-2-ol mixture and subsequent derivatization with CH3ONa/BF3. The limits of detection and recoveries for 18:2n-6, 20:4n-6, 18:3n-3, 20:5n-3, 22:5n-3 and 22:6n-3 fatty acids was in the range 0.32–0.42 mg/100 g, and 83–105 %, respectively. The content of physiologically important icosapentaenoic acid (EPA) in chicken breast meat, thigh meat, quail eggs and cricket nymphs was 3, 10, 51 and 21 mg/100 g of fresh matter (P < 0.05). The EPA contents in livers of laboratory rats fed diets supplemented with palm oil, safflower oil and fish oil was 4, 3 and 342 mg/100 g, respectively (P < 0.05).
- MeSH
- Cholesterol metabolism MeSH
- Chromatography, Gas * methods instrumentation utilization MeSH
- Animal Experimentation MeSH
- Cardiovascular Diseases prevention & control MeSH
- alpha-Linolenic Acid analysis MeSH
- Eicosapentaenoic Acid analysis MeSH
- Docosahexaenoic Acids analysis MeSH
- Humans MeSH
- Lipids analysis MeSH
- Fatty Acids, Unsaturated * analysis MeSH
- Tissue Extracts analysis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Technical polychlorinated byphenyl (PCB) preparations--Delors 103, 104, 105, and 106--produced in the former Czechoslovakia were analyzed for their chlorobiphenyl (CB), chloronaphthalene (PCN) and chlorinated dibenzofuran (PCDF) composition and content using high resolution gas chromatography-mass spectrometry techniques. The congener patterns of Delors 103, 104, 105, and 106 resembled Aroclors 1242, 1248, 1254, and 1260. Delors contained PCNs and PCDFs, as impurities, at microgram per gram concentrations. Concentrations of PCNs and PCDFs in Delors were greater than those found in the corresponding Aroclors. The potential for the emissions of PCNs and PCDFs from Delor was estimated to be 3680 and 860 kg, respectively. Non- and mono-ortho PCBs were the major contributors to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents in Delor mixtures.
- MeSH
- Benzofurans analysis MeSH
- Isomerism MeSH
- Environmental Pollutants analysis MeSH
- Naphthalenes analysis MeSH
- Gas Chromatography-Mass Spectrometry methods MeSH
- Polychlorinated Biphenyls analysis chemistry MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
