- MeSH
- dospělí MeSH
- geny erbB-2 genetika MeSH
- hybridizace in situ fluorescenční využití MeSH
- imunohistochemie metody využití MeSH
- lidé MeSH
- nádory prsu genetika mortalita MeSH
- prognóza MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- MeSH
- diagnostické techniky urologické využití MeSH
- diferenciální diagnóza MeSH
- financování organizované MeSH
- karcinom z renálních buněk diagnóza MeSH
- lékařská onkologie metody trendy MeSH
- lidé MeSH
- nádory ledvin diagnóza MeSH
- nádory podle histologického typu MeSH
- papilární karcinom diagnóza MeSH
- protoonkogenní proteiny c-kit diagnostické užití izolace a purifikace MeSH
- Check Tag
- lidé MeSH
Mutation and/or loss of the TP53 tumour suppressor gene is the single most common genetic abnormality in human cancer. The majority of TP53 mutations lead to stabilization of the protein, so that immunohistochemical staining for p53 can suggest mutation status in many cases. However, various false-positive and false-negative situations mean that simple immunostaining for p53 is not informative in a substantial number of tumours. In the present study, a series of 119 human cancers were immunostained using a highly sensitive technique that detects the low levels of wild-type protein expressed in normal cells, such that homozygous gene deletion or non-sense TP53 mutation can be identified by an absence of staining. TP53 gene status was also assessed using FASAY as a genetic/functional screen and in selected cases by direct sequencing. A quantitative scoring system was employed to assess p53 levels, and p53 post-translational modification was evaluated using antibodies that detect specific phosphorylation sites. Phosphorylated p53 correlated with total p53 levels and did not improve the prediction of TP53 mutation status. The transcriptional activity of TP53 was determined by staining for two downstream target genes, p21(WAF1) and MDM2, and statistical correlations between MDM2/p21(WAF1) and p53 were found in tumours with wild-type, but not mutant TP53. Measurement of staining for p53 and MDM2 accurately identifies the TP53 status of tumours. This simple and cost-effective method, applicable to automated staining and quantitation methods, improves the identification of TP53 status over standard methods for p53 immunostaining and provides information about tumour p53 phenotype that is complementary to genotyping data. Copyright (c) 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
- MeSH
- adenokarcinom genetika patologie MeSH
- antigen Ki-67 genetika MeSH
- DNA nádorová genetika MeSH
- financování organizované MeSH
- fosforylace MeSH
- genetická transkripce imunologie MeSH
- geny p53 genetika MeSH
- imunohistochemie metody MeSH
- inhibitor p21 cyklin-dependentní kinasy genetika MeSH
- lidé MeSH
- mutace MeSH
- nádorový supresorový protein p53 genetika MeSH
- nádory děložního čípku genetika patologie MeSH
- nádory endometria genetika MeSH
- nádory prsu genetika patologie MeSH
- nádory vaječníků genetika patologie MeSH
- nádory genetika patologie MeSH
- protoonkogenní proteiny c-mdm2 genetika MeSH
- spinocelulární karcinom genetika patologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
