Nuclear magnetic resonance (NMR) metabolomics was used for identification of metabolic changes in pancreatic cancer (PC) blood plasma samples when compared to healthy controls or diabetes mellitus patients. An increased number of PC samples enabled a subdivision of the group according to individual PC stages and the construction of predictive models for finer classification of at-risk individuals recruited from patients with recently diagnosed diabetes mellitus. High-performance values of orthogonal partial least squares (OPLS) discriminant analysis were found for discrimination between individual PC stages and both control groups. The discrimination between early and metastatic stages was achieved with only 71.5% accuracy. A predictive model based on discriminant analyses between individual PC stages and the diabetes mellitus group identified 12 individuals out of 59 as at-risk of development of pathological changes in the pancreas, and four of them were classified as at moderate risk.
- MeSH
- diabetes mellitus * MeSH
- diskriminační analýza MeSH
- lidé MeSH
- magnetická rezonanční spektroskopie MeSH
- metabolomika * MeSH
- pankreas MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The association of pancreatic cancer with type 2 diabetes mellitus was investigated by 1H NMR metabolomic analysis of blood plasma. Concentration data of 58 metabolites enabled discrimination of pancreatic cancer (PC) patients from healthy controls (HC) and long-term type 2 diabetes mellitus (T2DM) patients. A panel of eight metabolites was proposed and successfully tested for group discrimination. Furthermore, a prediction model for the identification of at-risk individuals for future development of pancreatic cancer was built and tested on recent-onset diabetes mellitus (RODM) patients. Six of 59 RODM samples were assessed as PC with an accuracy of more than 80%. The health condition of these individuals was re-examined, and in four cases, a correlation to the prediction was found. The current health condition can be retrospectively attributed to misdiagnosed pancreatogenic diabetes or to early-stage pancreatic cancer.
- MeSH
- časná detekce nádoru MeSH
- diabetes mellitus 2. typu * diagnóza MeSH
- diabetes mellitus * MeSH
- lidé MeSH
- metabolomika MeSH
- nádory slinivky břišní * diagnóza MeSH
- protonová magnetická rezonanční spektroskopie MeSH
- retrospektivní studie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The medicinal plant Swertia multicaulis D. Don was collected in Rasuwa District (Nepal) and the xanthone content of its ethyl acetate extracts was studied. The total amount of xanthones in S. multicaulis determined by HPLC reaches almost 13 g of xanthones per 1 kg of dry matter. The identification of xanthones in S. multicaulis was achieved by a combination of HPLC, LC⁻MS and LC⁻NMR. The final assignment of the individual chemical structures was provided by NMR, supported by preparative HPLC. In eight chromatographic peaks, four major xanthones were identified—1,3-dihydroxy-5,8-dimethoxyxanthone, 1-hydroxy-3,5,8-trimethoxyxanthone, bellidifolin (1,5,8-tri-hydroxy-3-methoxyxanthone), and decussatin (1-hydroxy-3,7,8-trimethoxyxanthone).
- MeSH
- chromatografie kapalinová MeSH
- fytonutrienty analýza chemie MeSH
- magnetická rezonanční spektroskopie MeSH
- molekulární struktura MeSH
- rostlinné extrakty analýza chemie MeSH
- sekundární metabolismus MeSH
- Swertia chemie metabolismus MeSH
- tandemová hmotnostní spektrometrie MeSH
- xantony analýza chemie MeSH
- Publikační typ
- časopisecké články MeSH
Metabolic changes induced by pancreatic cancer were investigated by 1H NMR spectroscopy of plasma samples of patients and healthy controls. The acquired data were submitted to multivariate statistical analysis providing clear discrimination between both groups. The most significant differences were found in levels of 3-hydroxybutyrate and lactate. The obtained results (100% sensitivity, 90% specificity) clearly show the potential of 1H NMR spectroscopy in pancreatic cancer diagnosis. Therefore, the NMR-based metabolomics may contribute to the early diagnosis, prevention and/or therapy of diseases in the future. On the other hand, the number of samples in the presented pilot study is limited and has to be significantly increased in the future in order to obtain solid statistical models and to confirm the current findings.
- MeSH
- aminokyseliny krev MeSH
- krev metabolismus MeSH
- kyselina 3-hydroxymáselná krev MeSH
- kyselina mléčná krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- metabolomika metody MeSH
- multivariační analýza MeSH
- nádorové biomarkery krev MeSH
- nádory slinivky břišní krev diagnóza MeSH
- protonová magnetická rezonanční spektroskopie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol.
- MeSH
- časové faktory MeSH
- chemická frakcionace metody MeSH
- hole * MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- polyfenoly chemie izolace a purifikace MeSH
- rostlinné extrakty chemie izolace a purifikace MeSH
- rozpouštědla MeSH
- stilbeny chemie izolace a purifikace MeSH
- teplota MeSH
- Vitis chemie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- fyziologie výživy MeSH
- lidé MeSH
- socialismus MeSH
- Check Tag
- lidé MeSH
- MeSH
- fyziologie výživy MeSH
- lidé MeSH
- socialismus MeSH
- Check Tag
- lidé MeSH
