The viability, growth and morphology of 48 strains of Ascomycota (including 17 yeasts) and 20 strains of Zygomycota were determined after a 2-d and then after 1-year storage in liquid nitrogen using a new cryopreservation method with perlite as a particulate solid carrier. In case of Ascomycota, 45 strains (94 %) out of 48 survived both 2-d and 1-year storage in liquid nitrogen, respectively. In case of Zygomycota, all 20 strains survived both storage. In addition, 3 strains of Basidiomycota counted among yeasts were tested and all survived the 1 year storage. In all surviving cultures no negative effects of cryopreservation by this method have been observed after 1-year of storage in liquid nitrogen. The results indicate that the perlite protocol can be successfully used for cryopreservation of taxonomically different groups of fungi and also for fungi which failed to survive other routinely used preservation procedures.
- MeSH
- buněčná membrána metabolismus MeSH
- delece genu MeSH
- fenotyp MeSH
- finanční podpora výzkumu jako téma MeSH
- genetické vektory MeSH
- proteiny farmakologie genetika metabolismus MeSH
- Saccharomyces cerevisiae - proteiny genetika metabolismus MeSH
- Saccharomyces cerevisiae genetika metabolismus MeSH
- transformace genetická MeSH
- vakuoly metabolismus MeSH
Formation of organized colony morphology is clearly a result of organized, coordinated behavior of cells within a colony, which reflects changes in the cell environment, nutrient availability, inter- and intracolony signaling and others. Under standard conditions, colony morphology is specific to the particular yeast strain, which indicates that reproducibility of the structure appears to be a hallmark of programmed development. Our data indicate that markedly structured morphology of colonies formed by some haploid and diploid Saccharomyces cerevisiae strains is linked to formation of clusters of incompletely separated yeast cells organized into larger aggregates. A prerequisite to aggregate formation appears to be a monopolar budding pattern and the presence of an extracellular matrix and adhesins connecting individual clusters within aggregates. In contrast, less structured colonies of other strains are composed of non-aggregated cells exhibiting different modes of budding (axial/polar/random) in different colony areas. The budding pattern appears to be influenced more by the environment (the presence of surrounding cells, nutrient gradients, etc.) than by strain ploidy. Disruption of the BUD2 gene results in uniform random budding of cells and it partially influences colony morphology.
- MeSH
- buněčná adheze MeSH
- buněčné dělení MeSH
- financování organizované MeSH
- fungální proteiny metabolismus MeSH
- GTP-fosfohydrolasy fyziologie genetika MeSH
- mikrofotografie MeSH
- morfogeneze MeSH
- proteiny aktivující GTPasu MeSH
- Saccharomyces cerevisiae - proteiny fyziologie genetika MeSH
- Saccharomyces cerevisiae cytologie fyziologie MeSH
