The viability, growth and morphology of 48 strains of Ascomycota (including 17 yeasts) and 20 strains of Zygomycota were determined after a 2-d and then after 1-year storage in liquid nitrogen using a new cryopreservation method with perlite as a particulate solid carrier. In case of Ascomycota, 45 strains (94 %) out of 48 survived both 2-d and 1-year storage in liquid nitrogen, respectively. In case of Zygomycota, all 20 strains survived both storage. In addition, 3 strains of Basidiomycota counted among yeasts were tested and all survived the 1 year storage. In all surviving cultures no negative effects of cryopreservation by this method have been observed after 1-year of storage in liquid nitrogen. The results indicate that the perlite protocol can be successfully used for cryopreservation of taxonomically different groups of fungi and also for fungi which failed to survive other routinely used preservation procedures.
- MeSH
- biomedicínský výzkum metody MeSH
- dermatózy nohy (od hlezna dolů) etiologie patologie MeSH
- dýchání MeSH
- houby * metabolismus patogenita růst a vývoj MeSH
- kultivační techniky metody využití MeSH
- kůže * mikrobiologie parazitologie MeSH
- lidé MeSH
- mycelium metabolismus růst a vývoj MeSH
- statistika jako téma MeSH
- struktury hub metabolismus patogenita růst a vývoj MeSH
- tinea pedis MeSH
- Trichophyton * izolace a purifikace metabolismus růst a vývoj MeSH
- Check Tag
- lidé MeSH
