Caspases have functions particularly in apoptosis and inflammation. Increasing evidence indicates novel roles of these proteases in cell differentiation, including those involved in osteogenesis. This investigation provides a complex screening of osteogenic markers affected by pan caspase inhibition in micromass cultures derived from mouse forelimbs. PCR Array analysis showed significant alterations in expression of 49 osteogenic genes after 7 days of inhibition. The largest change was a decrease in CD36 expression, which was confirmed at organ level by caspase inhibition in cultured mouse ulnae followed by CD36 immunohistochemical analysis. So far, available data point to osteogenic potential of pro-apoptotic caspases. Therefore, the expression of pro-apoptotic caspases (-3, -6, -7, -8, -9) within the growth plate of mouse forelimbs at the stage where the individual zones are clearly apparent was studied. Caspase-9 was reported in the growth plate for the first time as well as caspase-6 and -7 in the resting zone, caspase-7 in the proliferation, and caspase-6 and -8 in the ossification zone. For all caspases, there was a gradient increase in activation toward the ossification zone. The distribution of staining varied significantly from that of apoptotic cells, and thus, the results further support non-apoptotic participation of caspases in osteogenesis.
- MeSH
- antigeny CD36 analýza genetika MeSH
- imunohistochemie MeSH
- inhibitory kaspas farmakologie MeSH
- kaspasy metabolismus MeSH
- kultivované buňky MeSH
- myši MeSH
- orgánové kultury - kultivační techniky MeSH
- osteogeneze * účinky léků MeSH
- přední končetina růst a vývoj metabolismus MeSH
- vývojová regulace genové exprese účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Analysing the chemical content of individual cells has already been proven to reveal unique information on various biological processes. Single-cell analysis provides more accurate and reliable results for biology and medicine than analyses of extracts from cell populations, where a natural heterogeneity is averaged. To meet the requirements in the research of important biologically active molecules, such as caspases, we have developed a miniaturized device for simultaneous analyses of individual cells. A stainless steel body with a carousel holder enables high-sensitivity parallel detections in eight microvials. The holder is mounted in front of a photomultiplier tube with cooled photocathode working in photon counting mode. The detection of active caspase-3/7, central effector caspases in apoptosis, in single cells is based on the bioluminescence chemistry commercially available as Caspase-Glo®3/7 reagent developed by Promega. Individual cells were captured from a culture medium under microscope and transferred by micromanipulator into detection microvial filled with the reagent. As a result of testing, the limits of detection and quantification were determined to be 0.27/0.86 of active caspase-3/7 content in an average apoptotic cell and 0.46/2.92 for non-apoptotic cells. Application potential of this technology in laboratory diagnostics and related medical research is discussed. Graphical abstract Miniaturized device for simultaneous analyses of individual cells.
- MeSH
- analýza jednotlivých buněk přístrojové vybavení metody MeSH
- apoptóza * MeSH
- design vybavení MeSH
- enzymatické testy přístrojové vybavení metody MeSH
- kaspasa 3 analýza metabolismus MeSH
- kaspasa 7 analýza metabolismus MeSH
- kultivované buňky MeSH
- luminiscenční měření přístrojové vybavení metody MeSH
- myši MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Caspases, well-known players in apoptosis or inflammation, appear to have roles also in other processes such as cell differentiation. Caspase-3, in particular, was recently demonstrated to have non-apoptotic functions in osteogenesis. However, the molecular pathways involved are not yet known. Therefore, we used osteogenic PCR arrays to provide a comprehensive screening of possible interactions of caspases in general and specifically of caspase-3 in osteogenic networks. Embryonic micromass cultures derived from mouse forelimbs were established and pharmacological fluoromethylketone (FMK) inhibitors applied. Alterations were observed in expression of several genes after caspase inhibition (Bmp1, Bmp5, Bmp6, Col10a1, Col2a1, Comp, Egf, Fgfr2, Gli1, Igf1, Nog, Phex, Sox9, Spp1). The list suggests molecular interactions of caspases and osteogenic molecules and creates a background for further temporospatial and functional studies.
- MeSH
- apoptóza účinky léků MeSH
- buněčná diferenciace účinky léků MeSH
- chondrogeneze účinky léků MeSH
- inhibitory kaspas aplikace a dávkování MeSH
- kaspasa 3 genetika metabolismus MeSH
- mezenchymální kmenové buňky účinky léků MeSH
- myši MeSH
- osteogeneze účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Vydání první 192 stran : formuláře ; 21 cm
Právo
1. vydání 350 stran : formuláře ; 21 cm
- MeSH
- sociální práce zákonodárství a právo MeSH
- Publikační typ
- příručky MeSH
- Geografické názvy
- Česká republika MeSH
- Konspekt
- Druhy sociální pomoci a služeb
- NLK Obory
- sociologie
- právo, zákonodárství
- NLK Publikační typ
- právní předpisy
- Klíčová slova
- HIV, AIDS,
- MeSH
- poradenství MeSH
- sociální práce MeSH
