Lymeská borrelióza je v dnešní době jednou z nejrozšířeně jších zoonóz. Přenašečem tohoto onemocnění jsou klíšťata. Symptomy lymeské borreliózy jsou velmi různorodé a nespecifické, často je lze zamě nit s jinými infekč ními onemocněními. Diagnostika je založena na posouzení klinických projevů spolu s laboratorním vyšetřením různých biologických materiálů . Problém v diagnostice přináší nestandardizované laboratorní testy. V posledních letech je pozornost v ě nována chronickému stádiu lymeské borreliózy. Tato fáze onemocn ě ní je dlouhodob ě lé č ena antibiotiky. Proto se hledalo vhodné laboratorní vyšet ř ení, které by pomohlo zhodnotit efektivitu a ukon č ení terapie. Podle n ě kterých pozorování se jevil jako vhodný test stanovení po č tu CD57+NK bun ě k. Tento č lánek p ř ináší souhrn sou č asných poznatk ů o lymeské borrelióze a CD57+NK bu ň kách.
Presently, Lyme borreliosis is one of the most widespread zoonosis and is spread by ticks. Symptoms of the borreliosis are misc ellaneous, not specific and moreover, the symptoms can be often classified as symptoms of other different infectious diseases. Lyme borreliosi s is diagnosed clinically based on symptoms and objective physical findings as well as laboratory tests. Problems with diagnostics are caused by non-standardized laboratory tests. Recently, the chronical phase of Lyme borreliosis has been examined. The chronical phase is cured by antibiot ics and thus effective test has been sought. Determination of CD57+NK cells seems to be appropriate test. The thesis brings a review on diag nosis and treatment of Lyme borreliosis and CD57+NK cells.
- MeSH
- akutní nemoc MeSH
- antibakteriální látky terapeutické užití MeSH
- antigeny CD57 * analýza imunologie MeSH
- Borrelia burgdorferi imunologie patogenita MeSH
- buňky NK * imunologie MeSH
- chronická nemoc MeSH
- ELISA MeSH
- fluorescenční protilátková technika MeSH
- lidé MeSH
- lymeská nemoc * diagnóza imunologie MeSH
- počet buněk MeSH
- polymerázová řetězová reakce MeSH
- protilátky bakteriální analýza imunologie MeSH
- western blotting MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Urobilinoids belong to the heterogenous group of degradation products of bilirubin formed in the gastrointestinal tract by intestinal microflora. Among them urobilinogen and stercobilinogen with their respective oxidation products, urobilin and stercobilin, are the most important compounds. The aim of present study was to analyze the products of bacterial reduction of bilirubin in more detail. The strain of Clostridium perfringens isolated from neonatal stools, capable of reducing bilirubin, was used in the study. Bacteria were incubated under anaerobic conditions with various native as well as synthetic bile pigments, including radiolabeled unconjugated bilirubin (UCB). Their reduction products were extracted from media and separated following thin layer chromatography. Pigments isolated were analyzed by spectrophotometry, spectrofluorometry and mass spectrometry. In a special set of experiments, bilirubin diglucuronide was incubated with either bacterial lysate or partially purified bilirubin reductase and beta-glucuronidase to reveal whether bilirubin glucuronides may be directly reduced onto conjugated urobilinoids. A broad substrate activity was detected in the investigated strain of C. perfringens and a series of bilirubin reduction products was identified. These products were separated in the form of their respective chromogens and further oxidized. Based on their physical-chemical properties, as well as mass spectra, end-catabolic bilirubin products were identified to belong to urobilinogen species. The reduction process, catalyzed enzymatically by the studied bacterial strain, does not proceed to stercobilinogen. Bilirubin diglucuronide is not reduced onto urobilinoid conjugates, glucuronide hydrolysis must precede double bond reduction and thus UCB is reduced much faster.
- MeSH
- bilirubin metabolismus MeSH
- chromatografie na tenké vrstvě MeSH
- Clostridium perfringens izolace a purifikace metabolismus MeSH
- feces mikrobiologie MeSH
- financování organizované MeSH
- hmotnostní spektrometrie MeSH
- lidé MeSH
- oxidace-redukce MeSH
- spektrofotometrie ultrafialová MeSH
- Check Tag
- lidé MeSH
