1. elektronické vydání 1 online zdroj (166 stran)
Kniha nabízí sto otázek a sto odpovědí o Antarktidě. V několika oddílech autoři, účastníci antarktických výzkumů, představují tento kontinent, historii jeho objevování, geologii, věnují se klimatologickým otázkám a dopadům globálního oteplování v Antarktidě. V částech z biologických věd pojednávají o živočiších, včetně mikroorganismů, a rostlinách Antarktidy. Závěr věnují českému antarktickému výzkumnému programu, zajímavostem z české stanice J. G. Mendela, informacím o jejím budování a provozu od roku 2006 i samotnému ostrovu Jamese Rosse.; Kniha nabízí sto otázek a sto odpovědí o Antarktidě.V několika oddílech autoři, účastníci antarktických výzkumů, představují tento kontinent, historii jeho objevování, geologii, věnují se klimatologickým otázkám a dopadům globálního oteplování v Antarktidě. V částech z biologických věd pojednávají o živočiších, včetně mikroorganismů, a rostlinách Antarktidy. Závěr věnují českému antarktickému výzkumnému programu, zajímavostem z české stanice J. G. Mendela, informacím o jejím budování a provozu od roku 2006 i samotnému ostrovu Jamese Rosse.
A taxonomic study of two fluorescent Pseudomonas strains (HJ/4T and SJ/9/1T) isolated from calcite moonmilk samples obtained from two caves in the Moravian Karst in the Czech Republic was carried out. Results of initial 16S rRNA gene sequence analysis assigned both strains into the genus Pseudomonas and showed Pseudomonas yamanorum 8H1T as their closest neighbour with 99.8 and 99.7 % 16S rRNA gene similarities to strains HJ/4T and SJ/9/1T, respectively. Subsequent sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of both isolates to P. yamanorum 8H1T, but phylogeny and sequences similarities implied that they are representatives of two novel species within the genus Pseudomonas. Further study comprising whole-genome sequencing followed by average nucleotide identity and digital DNA-DNA hybridization calculations, repetitive sequence-based PCR fingerprinting with the REP and ERIC primers, automated ribotyping with the EcoRI restriction endonuclease, cellular fatty acid analysis, quinone and polar lipid characterization, and extensive biotyping confirmed clear separation of both analysed strains from the remaining Pseudomonas species and showed that they represent two novel species within the genus Pseudomonas for which the names Pseudomonas karstica sp. nov. (type strain HJ/4T=CCM 7891T=LMG 27930T) and Pseudomonas spelaei sp. nov. (type strain SJ/9/1T=CCM 7893T=LMG 27931T) are suggested.
- MeSH
- bakteriální geny MeSH
- DNA bakterií genetika MeSH
- fylogeneze * MeSH
- hybridizace nukleových kyselin MeSH
- jeskyně mikrobiologie MeSH
- lipidy analýza MeSH
- mastné kyseliny chemie MeSH
- Pseudomonas klasifikace MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- techniky typizace bakterií MeSH
- uhličitan vápenatý * MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
West Nile virus lineage 2 (WNV-2) was detected in the brain of 17 goshawks (Accipiter gentilis) that succumbed to neuroinvasive disease in the Czech Republic during 2018: twelve birds were captive and five wild. Furthermore, two wild sparrowhawks (Accipiter nisus) and three other captive birds of prey (golden eagle Aquila chrysaetos, hybrid saker falcon Falco cherrug × F. rusticolus and Harris's hawk Parabuteo unicinctus) also died due to WNV encephalitis. The 2018 outbreak in Czech raptors clearly reflects a new epidemiological situation and indicates an increasing risk of both raptor and human infection with WNV-2 in the country.
- MeSH
- epidemický výskyt choroby * MeSH
- některé rody čeledi Accipitridae mikrobiologie MeSH
- nemoci ptáků epidemiologie virologie MeSH
- veřejné zdravotnictví MeSH
- virus západního Nilu genetika MeSH
- západonilská horečka epidemiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
In 2017, we isolated and identified West Nile virus (WNV) lineage 2 from two dead captive goshawks (Accipiter gentilis), for the first time in the Czech Republic. Goshawk might serve as an early indicator species for the ongoing WNV emergence in several European countries.
BACKGROUND: Salmonella enterica serovar Typhimurium is one of the most common enteropathogenic bacteria found in pigs in Europe. In our previous work, we demonstrated the protective effects in suckling piglets when their dams had been vaccinated with an S. Typhimurium-based inactivated vaccine. This study is focused on a procedure leading to serological discrimination between vaccinated and infected pigs. As we supposed, distinct environment during natural infection and in bacterial cultures used for vaccine preparation led to a slightly different spectrum of expressed S. Typhimurium proteins. The examination of porcine antibodies produced after the experimental infection with S. Typhimurium or after vaccination with S. Typhimurium-based inactivated vaccine by affinity chromatography and mass spectrometry revealed differences in antibody response applicable for serological differentiation of infected from vaccinated animals. RESULTS: Antibodies against Salmonella SipB, SipD and SseB proteins were detected at much higher levels in post-infection sera in comparison with control and post-vaccination sera. On the other hand, proteins BamB, OppA and a fragment of FliC interacted with antibodies from post-vaccination sera with a much higher intensity than from control and post-infection sera. In addition, we constructed ELISA assays using post-infection antigen - SipB protein and post-vaccination antigen - FliC-fragment and evaluated them on a panel of individual porcine sera. CONCLUSIONS: The analysis of antibody response of infected and vaccinated pigs by proteomic tools enabled to identify S. Typhimurium antigens useful for distinguishing infected from vaccinated animals. This approach can be utilized in other challenges where DIVA vaccine and a subsequent serological assay are required, especially when genetic modification of a vaccine strain is not desirable.
- MeSH
- antigeny bakteriální metabolismus MeSH
- ELISA veterinární MeSH
- inaktivované vakcíny imunologie MeSH
- nemoci prasat diagnóza imunologie MeSH
- prasata MeSH
- proteomika * MeSH
- protilátky bakteriální krev MeSH
- Salmonella typhimurium genetika MeSH
- salmonelová infekce u zvířat diagnóza imunologie MeSH
- salmonelové vakcíny imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
During the microbiological research performed within the scope of activities of Czech expeditions based at the Johann Gregor Mendel Station at James Ross Island, Antarctica, two psychrotrophic gram-stain negative non-fluorescent strains CCM 8506T and CCM 8507 from soil were extensively characterized using genotypic and phenotypic methods. Initial characterization using ribotyping with HindIII restriction endonuclease and phenotyping implies that both isolates belong to a single Pseudomonas species. Sequencing of rrs, rpoB, rpoD and glnA genes of strain CCM 8506(T) confirmed affiliation of investigated strains within the genus Pseudomonas. Further investigation using automated ribotyping with EcoRI (RiboPrinter(®) Microbial Characterisation System), whole-cell protein profiling using the Agilent 2100 Bioanalyzer system, extensive biochemical testing and DNA-DNA hybridization experiments confirmed that both investigated strains are members of a single taxon which is clearly separated from all hitherto described Pseudomonas spp. Based on all findings, we describe a novel species Pseudomonas gregormendelii sp. nov. with the type strain CCM 8506(T) (=LMG 28632T).
- MeSH
- bakteriální proteiny genetika metabolismus MeSH
- DNA bakterií genetika MeSH
- fylogeneze MeSH
- Pseudomonas klasifikace genetika izolace a purifikace metabolismus MeSH
- půdní mikrobiologie * MeSH
- ribozomální DNA genetika MeSH
- RNA ribozomální 16S genetika MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Antarktida MeSH
Pseudomonas monteilii CCM 3423 bacterial strain, deposited at the Czech Collection of Microorganisms, was originally isolated by Haľama and Augustín (1980) as a bacterium degrading aromatic hydrocarbons and derivates. A detailed study supported by a molecular genetics method of sequence analyses of rrs and rpoD genes was used to reclassify the strain, originally stored as 'Pseudomonas putida'. The physiological characteristics of the strain are complemented with research in the capacity to utilize selected organic pollutants (anthracene, benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, fluorene, naphthalene, phenanthrene). The obtained results point at very good biodegradation properties of the strain. Already after 7 days of the bacterial strain's action, there was a decrease in all the organic contaminants to 79.8 ± 2.6 %. In 14 days, the amount of organic contaminants dropped to 59.3 ± 2.8 %. After 21 days of biodegradation experiments, the overall quantity of the observed organic substances fell below the half limit to 45.7 ± 2.5 % of residuals. Finally, after 28 days, the residue was 35.4 ± 2.2 %, and after 35 days of the action of P. monteilii, the tested samples contained mere 27.8 ± 2.8 % of organic pollutants. The results imply that Pseudomonas monteilii CCM 3423 is a prospective strain in terms of further biotechnological application in contaminated environment.
- MeSH
- biodegradace MeSH
- bronchy mikrobiologie MeSH
- fylogeneze MeSH
- látky znečišťující životní prostředí metabolismus MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- polycyklické aromatické uhlovodíky metabolismus MeSH
- Pseudomonas klasifikace genetika izolace a purifikace metabolismus MeSH
- regenerace a remediace životního prostředí přístrojové vybavení metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
During Czech expeditions at James Ross Island, Antarctica, in the years 2007-2009, the bacterial diversity of the genus Pseudomonas was studied. Twelve fluorescent Pseudomonas strains were isolated from various samples and were subjected to a detailed taxonomic study. A polyphasic approach included genotypic and phenotypic analyses. The genotypic analysis involved sequencing of rrs, rpoB and rpoD genes, DNA-DNA hybridization (DDH) studies as well as manual ribotyping using HindIII endonuclease. The phenotypic characterization included conventional tests as well as biotyping using the Biolog system, protein profiling by SDS-PAGE, and MALDI-TOF MS analysis. Our taxonomic study revealed that all isolates belonged to the same Pseudomonas species with psychrotrophic growth not exceeding 37 °C. The cultures showed a unique position among the phylogenetically related pseudomonads. DDH experiment between the proposed type strain of the antarctic isolates and the closest neighbour P. arsenicoxydans CCM 8423(T) showed only 40.9-50.1 % similarity, thus confirming that the characterized strains do not belong to the P. arsenicoxydans species. According to the results obtained we propose the name P. prosekii sp. nov. for this novel Pseudomonas taxon with type strain AN/28/1(T) (=CCM 7990(T) and LMG 26867(T)).
- MeSH
- DNA bakterií chemie genetika MeSH
- DNA řízené RNA-polymerasy genetika MeSH
- fylogeneze MeSH
- hybridizace nukleových kyselin MeSH
- mikrobiologie životního prostředí * MeSH
- molekulární sekvence - údaje MeSH
- Pseudomonas klasifikace genetika izolace a purifikace fyziologie MeSH
- ribotypizace MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- sigma faktor genetika MeSH
- techniky typizace bakterií MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Antarktida MeSH
A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all members of the genus Enterococcus with validly published names and placed them in the Enterococcus faecalis species group. DNA-DNA hybridization experiments, pheS and rpoA sequencing and whole-cell protein electrophoresis provided conclusive evidence for the classification of each phenon as a novel species of the genus Enterococcus, for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629(T) = LMG 26676(T) = CCUG 48799(T)), inhabiting water and plants, and Enterococcus rotai sp. nov. (type strain CCM 4630(T) = LMG 26678(T) = CCUG 61593(T)), inhabiting water, insects (mosquitoes) and plants, are proposed.
- MeSH
- bakteriální geny MeSH
- DNA bakterií genetika MeSH
- Enterococcus klasifikace genetika izolace a purifikace MeSH
- fylogeneze * MeSH
- hybridizace nukleových kyselin MeSH
- mikrobiologie životního prostředí MeSH
- molekulární sekvence - údaje MeSH
- pitná voda mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- techniky typizace bakterií MeSH
- ureasa biosyntéza MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Fluorescent Pseudomonas putida CCM 3656 (ATCC 11250) was analysed according to the methods of polyphasic approach which were based on sequence analyses involving the rpoB and rrs genes, manual ribotyping using endonuclease HindIII, DNA base composition determination and DNA-DNA hybridization. The results obtained by these genotyping methods showed that the strain CCM 3656 is distant from P. putida taxon, which was supported with phenotype characterization represented by whole-cell protein profile analysis, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry profiling and extended biotyping. The DNA-DNA hybridization experiments performed between the strain CCM 3656 and the closest relatives revealed 77 % similarity with Pseudomonas jessenii. However, the outcomes of sequencing, ribotyping and phenotype characterization allow distinguishing the studied strain from P. jessenii. On the basis of the obtained taxonomic data, we suggest reclassifying strain CCM 3656 to a novel subspecies of P. jessenii and propose naming P. jessenii subsp. pseudoputida subsp. nov. with CCM 3656(T) as type strain. Furthermore, we present an amended description of P. jessenii and proposal of P. jessenii subsp. jessenii subsp. nov.
- MeSH
- DNA řízené RNA-polymerasy genetika MeSH
- fylogeneze MeSH
- hybridizace nukleových kyselin MeSH
- molekulární sekvence - údaje MeSH
- proteom analýza MeSH
- Pseudomonas chemie klasifikace genetika MeSH
- ribotypizace MeSH
- ribozomální DNA chemie genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- techniky typizace bakterií MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
