OBJECTIVES: The objective of the study was to assess the metabolic risk of excessive dietary iodine intake in ewes and neonatal lambs. DESIGN: Pregnant Šumava ewes received an experimental diet containing 3.1 mg iodine per kg of dietary dry matter in Group A (control, n=13, 6 ewes and 7 lambs) and 5.1 mg iodine per kg of dietary dry matter in Group B (experimental, n=12, 6 ewes, 6 lambs) for eight months. Iodine was administered to ewes as calcium iodate. TSH in blood serum; TT3, TT4, fT3, and fT4 in blood plasma were examined in both groups of ewes and lambs to assess the risks of iodine intake above the permitted limit, as it applies to thyroid gland activity. RESULTS: Group B ewes showed a significant increase in TSH and TT4 only on day 1 after parturition. The highest values of TT4, TT3, and fT3 in lambs were recorded on day 1 after birth. The lowest values of fT3 and fT4 in lambs were measured on day 60 after birth with no differences observed between the groups. In lambs of Group B the lower concentration of TSH until day 3 after birth was followed by a significant increase from day 10 after birth. CONCLUSION: Our results indicate a risk of postnatal hypothyroidism among lambs from pregnant and lactating ewes having a high iodine intake.
- MeSH
- hypotyreóza krev chemicky indukované MeSH
- jod toxicita MeSH
- laktace MeSH
- náhodné rozdělení MeSH
- novorozená zvířata MeSH
- ovce MeSH
- štítná žláza účinky léků fyziologie MeSH
- stopové prvky toxicita MeSH
- těhotenství MeSH
- thyreotropin krev MeSH
- thyroxin krev MeSH
- trijodthyronin krev MeSH
- zpožděný efekt prenatální expozice krev chemicky indukované MeSH
- zvířata MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Our research was focused on finding the optimal technique of cryopreserved mitral allograft implantation into the tricuspid valve position in a sheep experiment. We prepared 20 cryopreserved mitral valve allografts preoperatively. These were implanted into the tricuspid position in 20 sheep via right thoracotomy, using extracorporeal circulation with cardioplegic heart arrest. We consider the transventricular papillary muscle fixation to be the best technique for obtaining optimal allograft geometry and long-term durability. We evaluated the valve function by epicardial echo before and after implantation, respectively. The findings were confirmed by haemodynamic measurements as well. It was proved that mitral allografts, harvested, processed, and stored according to the Cardiovascular Tissue Bank Protocol remained mechanically strong enough for implantation into the tricuspid position. It was demonstrated that the mitral allograft transplantation into the tricuspid position is feasible. The immediate postoperative haemodynamic performance was excellent in all animals. The mid-term results are currently being evaluated - prior to the possible application of the technique in humans at our institutions.
- MeSH
- alografty MeSH
- echokardiografie MeSH
- kryoprezervace MeSH
- mitrální chlopeň transplantace MeSH
- ovce MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- fagocytóza genetika MeSH
- granulocyty imunologie MeSH
- roční období MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- MeSH
- jod farmakologie MeSH
- kuřecí embryo účinky léků MeSH
- štítná žláza anatomie a histologie účinky léků MeSH
- velikost orgánu MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo účinky léků MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- feces chemie MeSH
- finanční podpora výzkumu jako téma MeSH
- krmivo pro zvířata klasifikace normy MeSH
- skot MeSH
- stopové prvky analýza MeSH
- Check Tag
- skot MeSH
- Publikační typ
- kongresy MeSH
