Učební texty Univerzity Karlovy v Praze
3. vydání 2 svazky : ilustrace ; 23 cm
Vysokoškolská učebnice, která se zaměřuje na lékařskou biochemii.
- MeSH
- biochemie MeSH
- klinická chemie MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- biochemie
- chemie, klinická chemie
- NLK Publikační typ
- učebnice vysokých škol
Gene therapy is often used in clinical research. It is related to the delivery of nucleic acids and their components to patient by a vector. Most current vector techniques can be divided into viral and nonviral. Nonviral vectors, such as cationic liposomes, are less toxic, less immunogenic, and easier to prepare than viral vectors. Hence they are more attractive for clinical applications. This review focuses on the relations between the cationic lipid structure and its transfection activity. To understand the structure-activity relationships is of great importance for synthesis of novel cationic lipids.
Učební texty Univerzity Karlovy v Praze
Vyd. 2. 1 sv. : il., tab. ; 23 cm
- MeSH
- biochemické jevy MeSH
- biochemie MeSH
- fyziologie výživy MeSH
- genetické struktury MeSH
- molekulární biologie MeSH
- MeSH zastaralé
- biochemické procesy MeSH
- genetické procesy MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- biochemie
- NLK Publikační typ
- učebnice vysokých škol
Učební texty Univerzity Karlovy v Praze
Vyd. 2. 2 sv. : il., tab. ; 23 cm
Učební texty Univerzity Karlovy v Praze
Vyd. 2. 269 s. : il. ; 23 cm
The need for safe and structurally defined immunomodulators and adjuvants is increasing in connection with the recently observed marked increase in the prevalence of pathological conditions characterized by immunodeficiency. Important groups of such compounds are muramyl glycopeptides, analogs of muramyl dipeptide (MDP), glucosaminyl-muramyl dipeptide (GMDP), and desmuramylpeptides. We have designed and synthesized new types of analogs with changes in both the sugar and the peptide parts of the molecule that show a high immunostimulating and adjuvant activity and suppressed adverse side effects. The introduction of lipophilic residues has also improved their incorporation into liposomes, which represent a suitable drug carrier. The proliposome-liposome method is based on the conversion of the initial proliposome preparation into liposome dispersion by dilution with the aqueous phase. The description of a home-made stirred thermostated cell and its link-up with a liquid delivery system for a rapid and automated preparation of multilamellar liposomes at strictly controlled conditions (sterility, temperature, dilution rate and schedule) is presented. The cell has been designed for laboratory-scale preparation of liposomes (300-1000 mg of phospholipid per run) in a procedure taking less than 90 min. The method can be readily scaled up. Examples of adjuvant and immunostimulatory effect of liposomal preparation in mice model will be presented.
Učební texty Univerzity Karlovy v Praze
Vyd. 1 2 sv. : tab., grafy ; 24 cm
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biochemie
- NLK Publikační typ
- učebnice vysokých škol
