Vysokoškolská učebnice, která se zaměřuje na stomatochirurgii, konkrétně na celkové zubní náhrady.

• MeSH
• celkové zubní protézy MeSH
• implantace zubů MeSH
• stomatochirurgie MeSH

• Konspekt
• Učební osnovy. Vyučovací předměty. Učebnice
• Stomatologie
• NLK Obory
• stomatochirurgie
• NLK Publikační typ
• učebnice vysokých škol

• Publikační typ
• abstrakt z konference MeSH

Úvod a cíl: Retrospektivní studie se zaměřila na klinicko-biopticky verifikované případy proliferativní verukózní leukoplakie (PVL). Cílem práce je poukázat na nutnost korelace orálních projevů PVL s klinickým, ale i rentgenologickým vyšetřením parodontu. Metodika: Studie hodnotila průběh onemocnění PVL u 53 pacientů sledovaných v letech 1994 až 2024 na Oddělení orální medicíny Stomatologické kliniky FN Plzeň. Mimo anamnesticko-patologické korelace byly posuzovány rovněž aspekty koincidence s dalšími lézemi ústní sliznice, tvorba dysplazie v průběhu tohoto agresivního onemocnění a četnost maligní transformace. Slizniční projev onemocnění byl pravidelně porovnáván s klinickým i rentgenologicky ověřeným stavem parodontu. Výsledky: Za dispenzarizace došlo k recidivě v 56 % případů (n = 30/53) a ke zvratu v orální dlaždicobuněčný karcinom (OSCC) u 38 % pacientů (n = 20/53). Onkologická recidiva nastala v pěti případech (9 %). Většinu pacientů s maligní transformací tvořily ženy, a to 70 % (n = 14/20). Závěr: Na rozdíl od ostatních onemocnění ústní sliznice je v rámci dispenzarizace PVL zhotovení snímků OPG nebo CBCT diagnosticky přínosnější. U mnoha pacientů s lézemi v oblasti alveolární sliznice a gingivy dochází v odpovídajících lokalitách k výrazné resorpci až usuraci alveolární kosti v případě probíhající maligní transformace. Maligní transformace PVL je skutečně četná, v naší skupině až v 38 %. Pro riziko diagnostické záměny orálního lichen planus a chronické hyperplastické kandidózy s počínající PVL je nutno zpřísnit dispenzární pravidla i u těchto případů.

Introduction and aim: Retrospective study focused on clinically and biopsy-proven cases of proliferative verrucous leukoplakia (PVL). The aim of the work is to point out the necessity of correlation of oral manifestations of PVL with clinical and radiological examination of the periodontium. Methods: The study evaluated the process of PVL disease in 53 patients followed between 1994 and 2024 at the Oral Medicine Department, Dentistry Clinic, University hospital in Pilsen. Apart from anamnestic-pathological correlations, also the aspects of coincidence with other lesions of the oral mucosa, the formation of dysplasia during this aggressive disease and the frequency of malignant transformation were assessed. The mucosal manifestation of the disease was regularly compared to the clinical and radiological state of the periodontium. Results: During dispensary, recurrence occurred in 56% of cases (n = 30/53) and transformation into oral squamous cell carcinoma (OSCC) in 38% of patients (n = 20/53). Oncological recurrence was in five cases (9%). The majority of patients with malignant transformation were women with 70% (n = 14/20). Conclusion: In contrary to other diseases of the oral mucosa, taking OPG or CBCT images is diagnostically more beneficial within the PVL dispensary. In several patients with lesions in the area of the alveolar mucosa and gingiva, there is significant resorption of the alveolar bone in the corresponding locations in the case of an ongoing malignant transformation. Malignant transformation of PVL is indeed high, up to 38% in our group. Due to the risk of diagnostic confusion of oral lichen planus and chronic hyperplastic candidiasis with incipient PVL, it is necessary to tighten the dispensary rules for these cases as well.

• MeSH
• dospělí MeSH
• karcinogeneze MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory úst patofyziologie   MeSH
• orální leukoplakie * diagnostické zobrazování   komplikace   patofyziologie   patologie   MeSH
• retrospektivní studie MeSH
• senioři MeSH
• spinocelulární karcinom patofyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• klinická studie MeSH

Paclitaxel (PTX) is a chemotherapeutic agent affecting microtubule polymerization. The efficacy of PTX depends on the type of tumor, and its improvement would be beneficial in patients' treatment. Therefore, we tested the effect of slow sulfide donor GYY4137 on paclitaxel sensitivity in two different breast cancer cell lines, MDA-MB-231, derived from a triple negative cell line, and JIMT1, which overexpresses HER2 and is resistant to trastuzumab. In JIMT1 and MDA-MB-231 cells, we compared IC50 and some metabolic (apoptosis induction, lactate/pyruvate conversion, production of reactive oxygen species, etc.), morphologic (changes in cytoskeleton), and functional (migration, angiogenesis) parameters for PTX and PTX/GYY4137, aiming to determine the mechanism of the sensitization of PTX. We observed improved sensitivity to paclitaxel in the presence of GYY4137 in both cell lines, but also some differences in apoptosis induction and pyruvate/lactate conversion between these cells. In MDA-MB-231 cells, GYY4137 increased apoptosis without affecting the IP3R1 protein, changing the morphology of the cytoskeleton. A mechanism of PTX sensitization by GYY4137 in JIMT1 cells is distinct from MDA-MB-231, and remains to be further elucidated. We suggest different mechanisms of action for H2S on the paclitaxel treatment of MDA-MB-231 and JIMT1 breast cancer cell lines.

• MeSH
• apoptóza * účinky léků   MeSH
• chemorezistence účinky léků   MeSH
• lidé MeSH
• morfoliny * farmakologie   MeSH
• nádorové buněčné linie MeSH
• nádory prsu * farmakoterapie   patologie   metabolismus   MeSH
• organothiofosforové sloučeniny * farmakologie   MeSH
• paclitaxel * farmakologie   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• sulfidy farmakologie   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The 3-mercaptopyruvate sulfurtransferase (MPST) is a protein persulfidase, occurring mainly in mitochondria. Although function of this protein in cancer cells has been already studied, no clear outcome can be postulated up to now. Therefore, we focused on the determination of function of MPST in colon (HCT116 cells)/colorectal (DLD1 cells) cancers. In silico analysis revealed that in gastrointestinal cancers, MPST together with its binding partners can be either of a high risk or might have a protective effect. Silencing of MPST gene resulted in decreased ATP, while acetyl-CoA levels were elevated. Increased apoptosis was detected in cells with silenced MPST gene, which was accompanied by decrease in mitochondrial membrane potential, but no changes in IP3 receptor's protein. Mitochondria underwent activation of fission and elevated DRP1 expression after MPST silencing. Proliferation and migration of DLD1 and HCT116 cells were markedly affected, showing the importance of MPST protein in colon/colorectal cancer development.

• MeSH
• apoptóza MeSH
• dynaminy metabolismus   genetika   MeSH
• HCT116 buňky MeSH
• kolorektální nádory * metabolismus   patologie   enzymologie   genetika   MeSH
• lidé MeSH
• membránový potenciál mitochondrií MeSH
• mitochondrie metabolismus   MeSH
• nádorové buněčné linie MeSH
• nádory tračníku metabolismus   patologie   genetika   enzymologie   MeSH
• pohyb buněk MeSH
• proliferace buněk MeSH
• sulfurtransferasy * metabolismus   genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Chronic hyperplastic candidiasis (CHC) presents a distinctive and relatively rare form of oral candidal infection characterized by the presence of white or white-red patches on the oral mucosa. Often mistaken for leukoplakia or erythroleukoplakia due to their appearance, these lesions display nonhomogeneous textures featuring combinations of white and red hyperplastic or nodular surfaces. Predominant locations for such lesions include the tongue, retro-angular mucosa, and buccal mucosa. This paper aims to investigate the potential influence of specific anatomical locations, retro-angular mucosa, on the development and occurrence of CHC. By examining the relationship between risk factors, we present an approach based on machine learning (ML) to predict the location of CHC occurrence. In this way, we employ Gradient Boosting Regression (GBR) to classify CHC lesion locations based on important risk factors. This estimator can serve both research and diagnostic purposes effectively. The findings underscore that the proposed ML technique can be used to predict the occurrence of CHC in retro-angular mucosa compared to other locations. The results also show a high rate of accuracy in predicting lesion locations. Performance assessment relies on Mean Squared Error (MSE), Root Mean Squared Error (RMSE), R-squared (R2), and Mean Absolute Error (MAE), consistently revealing favorable results that underscore the robustness and dependability of our classification method. Our research contributes valuable insights to the field, enhancing diagnostic accuracy and informing treatment strategies.

• Publikační typ
• časopisecké články MeSH

• Publikační typ
• abstrakt z konference MeSH

• Publikační typ
• abstrakt z konference MeSH

BACKGROUND: Cystathionine β-synthase (CBS), one of three enzymes that endogenously produce hydrogen sulfide, is extensively studied for its relevance in the cells of various tumors. In our previous work, we observed that the immunofluorescence pattern of CBS is very similar to that of tubulin and actin. Therefore, we focused on the potential interaction of CBS with cytoskeletal proteins β-actin and β-tubulin and the functional relevance of the potential interaction of these proteins in colorectal carcinoma cell lines. METHODS: To study the potential interaction of CBS with cytoskeletal proteins and its functional consequences, a CBS-knockout DLD1 (DLDx) cell line was established by using the CRISPR/Cas9 gene editing method. The interaction of the selected cytoskeletal protein with CBS was studied by immunoprecipitation, Western blot analysis, immunofluorescence, and proximity ligation assay. The functional consequences were studied by proliferation and migration assays and by generation of xenografts in SCID/bg mice. RESULTS: We have found that CBS, an enzyme that endogenously produces H2S, binds to cytoskeletal β-tubulin and, to a lesser extent, also to β-actin in colorectal carcinoma-derived cells. When CBS was knocked out by the CRISPR/Cas9 technique (DLDx), we observed a de-arranged cytoskeleton compared to the unmodified DLD1 cell line. Treatment of these cells with a slow sulfide donor GYY4137 resulted in normal organization of the cytoskeleton, thus pointing to the role of CBS in microtubule dynamics. To evaluate the physiological importance of this observation, both DLD1 and DLDx cells were injected into SCID/bg mice, and the size and mass of the developed xenografts were evaluated. Significantly larger tumors developed from DLDx compared to the DLD1 cells, which correlated with the increased proliferation of these cells. CONCLUSIONS: Taken together, in colorectal cancer DLD1 cells, CBS binds to the cytoskeleton, modulates microtubule dynamics, and thus affects the proliferation and migration in the colorectal carcinoma stable cell line.

• Publikační typ
• časopisecké články MeSH

• Publikační typ
• abstrakt z konference MeSH