In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.
- MeSH
- antioxidancia farmakologie izolace a purifikace MeSH
- dibenzyly farmakologie izolace a purifikace MeSH
- inhibitory lipoxygenas farmakologie izolace a purifikace MeSH
- isochinoliny farmakologie izolace a purifikace MeSH
- léčivé rostliny chemie MeSH
- peroxidace lipidů MeSH
- rostlinné extrakty farmakologie chemie MeSH
- MeSH
- druhová specificita MeSH
- esterasy metabolismus MeSH
- hydrolýza MeSH
- jaterní mikrozomy enzymologie MeSH
- krysa rodu rattus MeSH
- ledviny enzymologie MeSH
- mikrozomy metabolismus MeSH
- plíce enzymologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
