Our aim was to isolate bifidobacteria and clostridia from infant faeces and to test the growth of bifidobacteria and clostridia on prebiotic oligosaccharides. Seventy breast-fed infants aged between 3 and 253 days were tested for the presence of bifidobacteria and clostridia in their faeces. Ten strains of clostridia and 10 strains of bifidobacteria were isolated from infant faecal samples. Four strains of bifidobacteria originated from culture collections and 1 strain from fermented milk product were also tested. Subsequently, bacterial isolates were tested for their growth on prebiotic oligosaccharides in, in vitro conditions. Forty-six infants exhibited high numbers of bifidobacteria (usually higher than 9 logCFU/g) in their faeces. There were undetectable amounts of bifidobacteria in faecal samples in 24 of the studied infants (34%), these babies on the other hand possessed significant amounts of clostridia in their faecal flora. Both bifidobacteria and clostridia utilized all substrates tested. Bifidobacteria grew significantly better in the medium with galactooligosaccharides. Higher growth of clostridia was observed on raffinose and lactulose. Conversely, bifidobacteria grew slightly better in the medium with stachyose, inulin, Raftilose P85 and P95. However, these differences were not significant. Our results suggest that commercially available prebiotics support the growth of infant faecal clostridia. It is therefore questionable if bifidobacteria-deficient infants should be supplemented with prebiotics.
- MeSH
- Bifidobacterium izolace a purifikace metabolismus růst a vývoj MeSH
- Clostridium izolace a purifikace metabolismus růst a vývoj MeSH
- feces mikrobiologie MeSH
- financování organizované MeSH
- kojenec MeSH
- kysané mléčné výrobky MeSH
- lidé MeSH
- novorozenec MeSH
- oligosacharidy MeSH
- počet mikrobiálních kolonií MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- MeSH
- Bifidobacterium metabolismus růst a vývoj MeSH
- financování vládou MeSH
- gastrointestinální obsah mikrobiologie MeSH
- gastrointestinální trakt fyziologie mikrobiologie MeSH
- hybridizace in situ fluorescenční metody využití MeSH
- skot fyziologie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- skot fyziologie mikrobiologie MeSH
- zvířata MeSH
Sixty-four breast-fed infants and 23 calves were investigated for bacteria and enzymatic activity in their faecal samples. The bacteria were measured using cultivation and fluorescence in situ hybridization. Enzymatic activity was also examined. Forty-seven (64%) infants and all the calves had high numbers of bifidobacteria (usually >9 log CFU g-1) in their faeces, but 17 infants (36%) did not have a detectable amount of the bacteria. Most of the bifidobacteria-negative infants had significant quantities of clostridia in their faecal flora. While the infants did not have significantly higher counts of bifidobacteria, the samples from calves contained significantly (P<0.05) more coliform bacteria and lactobacilli. There were also significant differences in their enzymatic activities. Bifidobacteria-positive samples had a greater alpha-glucosidase activity, while bifidobacteria-negative samples had a lower activity of alpha-galactosidase, and calf samples had the highest beta-glucuronidase activity. A significant increase in bifidobacteria in calf faeces between days 3 and 7 was accompanied by a decrease in Escherichia coli. Our results show that the faecal flora of calves is similar to that of infants with regard to the occurrence of bifidobacteria as a dominant bacterial group.
- MeSH
- alfa-galaktosidasa metabolismus MeSH
- alfa-glukosidasy metabolismus MeSH
- beta-galaktosidasa metabolismus MeSH
- Bifidobacterium izolace a purifikace MeSH
- feces enzymologie mikrobiologie MeSH
- financování organizované MeSH
- hybridizace in situ fluorescenční MeSH
- lidé MeSH
- počet mikrobiálních kolonií MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- aldehydlyasy analýza MeSH
- Bifidobacterium enzymologie genetika izolace a purifikace MeSH
- dítě MeSH
- DNA bakterií genetika chemie MeSH
- elektroforéza MeSH
- feces mikrobiologie MeSH
- finanční podpora výzkumu jako téma MeSH
- glukuronidasa analýza MeSH
- glykosidhydrolasy analýza MeSH
- lidé MeSH
- novorozenec MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- novorozenec MeSH
